Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   N4Q31_RS05300 Genome accession   NZ_CP104391
Coordinates   1060151..1063660 (+) Length   1169 a.a.
NCBI ID   WP_106025297.1    Uniprot ID   -
Organism   Bacillus safensis strain GX-H6     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1055151..1068660
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N4Q31_RS05270 (N4Q31_05270) lepB 1055668..1056180 (+) 513 WP_106025301.1 signal peptidase I -
  N4Q31_RS05275 (N4Q31_05275) - 1056219..1057364 (-) 1146 WP_106025300.1 AbrB family transcriptional regulator -
  N4Q31_RS05280 (N4Q31_05280) - 1057632..1058054 (+) 423 WP_024425495.1 BlaI/MecI/CopY family transcriptional regulator -
  N4Q31_RS05285 (N4Q31_05285) - 1058047..1058886 (+) 840 WP_047202351.1 M56 family metallopeptidase -
  N4Q31_RS05290 (N4Q31_05290) - 1058944..1059354 (+) 411 WP_039177642.1 DoxX family protein -
  N4Q31_RS05295 (N4Q31_05295) - 1059552..1059998 (-) 447 WP_039177646.1 ferritin-like domain-containing protein -
  N4Q31_RS05300 (N4Q31_05300) addB 1060151..1063660 (+) 3510 WP_106025297.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  N4Q31_RS05305 (N4Q31_05305) addA 1063638..1067342 (+) 3705 WP_106025296.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  N4Q31_RS05310 (N4Q31_05310) sbcD 1067368..1068543 (+) 1176 WP_106025295.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1169 a.a.        Molecular weight: 134901.03 Da        Isoelectric Point: 5.4856

>NTDB_id=729414 N4Q31_RS05300 WP_106025297.1 1060151..1063660(+) (addB) [Bacillus safensis strain GX-H6]
MEIQFLAGRSGSGKTTAILEEIKEQLRLDPLGPPIIFLVPDQMTFLMEYELAKTSEAGGMIRAKVFSFTRLAWSILQQTG
GANRQFVTSTGIQMLLRKVIEEEKEKFKVFKKASDKPGFVEQIEKTMAEFKRYCMLPEDIEKISLSSMHSEYTEERRAGE
KLHDLHVLYQQMEQHLQDEYVHSEDYLTLLTQQIPLSEEIKGAHIYIDGFYQFTPQQLLVIEQLLLHAEKITAAFTVDRS
YHDKQPNELDLFRMTGKTYFQLYQLAKECGAAISEHILERNNRHLHTPDLAYLERQYEQRPVQPYQEVTPHLTVSKSASK
RAEIEGVAREILHLVREEGLRLRDITVVARHVDDYKDTLKEVFRDYDIPFFIDGNESMQYHPLIELIRSSLDVIKGNWRY
EAVFRCVKTEFLFPLELAKNKAREQADQLENYCIAYGVKGERWTNGSRFHYRRFQSLDEDFGQTDQEIEMEQMLNDVKEW
IAPPLFKLQKRLKKAQKVKDMVEAVYVFLEEIQVPDKLEKARLEAEEAGRLAEAMQHGQVWDAVIQLMDEFVEMLGEEEL
SFPLFQQMMDTGLASLKFALIPPSLDQVFIGSMDLSRMYQVKCMFIIGVNDGVIPARPSDESVLSEDDREWLKRAGAELA
ETGKERLLDEQFLIYQALSSPSHHLYLSYSVSDEEGRSLLPSTLIKYCQELMPNHQHALYVLDPEQLDDEEQLKFVANEH
VSLSYTVSQLQQWLNQYPISGVWWSVYNYLITSTHRDVSKNILSSLFFTNRAKRLKPNVTKELYGDHIQGSVSRMEKFNA
CAFSHFASHGLKLKDRQFYKLEAPDIGQLFHSALKHISDTLVEQKKDWKNLTKDDCVNYSRHAIEQLAPRLQKEILLSSN
RHAYIKEKLQKILIRVSSILSEHARVSGFSPVGLELGFGGQGPLPPFTFQLKNGCTMELVGRIDRVDKAEGSKGLLLRIV
DYKSSEKGLDLAEVYYGLALQMLTYLDLTITYSKEWLGVEATPAGILYFHIHDPLIQAPIPLAEDEIEQEIFKKFKMKGL
LLEDVEAVKLMDQTLESGRSQVIQAGLKKDGSFRSDSAVLSEDHFHILTKHVRRTFEEAGERITNGEIAINPYKLKDQTP
CRFCSFKSICQFDESIEDNEFRVLSSEKDDVVIDRIKKEGDQYANTKTE

Nucleotide


Download         Length: 3510 bp        

>NTDB_id=729414 N4Q31_RS05300 WP_106025297.1 1060151..1063660(+) (addB) [Bacillus safensis strain GX-H6]
GTGGAGATTCAGTTTTTAGCAGGCAGATCGGGGAGTGGAAAAACGACTGCAATCTTAGAGGAAATCAAAGAACAGCTTCG
GCTAGATCCGCTGGGTCCGCCAATCATTTTTTTAGTCCCGGATCAAATGACATTCTTAATGGAGTACGAGCTTGCAAAAA
CGTCTGAAGCTGGCGGGATGATCAGGGCCAAAGTATTTAGTTTCACGCGTCTTGCTTGGTCTATTTTACAGCAGACTGGC
GGAGCGAACCGGCAATTTGTGACAAGCACAGGAATTCAAATGCTTTTAAGAAAAGTAATTGAAGAAGAGAAAGAAAAGTT
TAAGGTATTCAAAAAAGCGAGCGATAAGCCGGGGTTTGTCGAGCAAATCGAAAAGACGATGGCTGAGTTCAAAAGATACT
GCATGCTGCCGGAGGATATTGAAAAAATTTCATTATCGAGCATGCATTCAGAGTATACAGAAGAAAGACGGGCAGGCGAA
AAATTGCATGATCTGCATGTTCTTTATCAGCAGATGGAGCAGCATTTACAAGATGAATATGTGCACTCAGAAGACTATTT
GACGCTTCTCACGCAGCAAATTCCTTTATCTGAAGAAATAAAAGGAGCACATATTTATATAGATGGCTTTTATCAATTTA
CACCGCAGCAGCTTCTCGTCATTGAACAGCTTCTGCTTCATGCTGAGAAAATAACGGCTGCGTTCACAGTGGATCGTTCC
TATCATGATAAGCAGCCAAATGAACTTGATTTATTTCGTATGACTGGTAAAACGTATTTCCAGCTCTATCAGCTTGCTAA
AGAGTGCGGAGCGGCCATTTCTGAGCACATTTTGGAGAGAAATAACCGACATCTTCATACGCCAGATCTTGCTTATTTAG
AACGCCAATATGAGCAGAGACCGGTGCAGCCATATCAGGAAGTAACCCCTCATCTCACAGTGTCTAAAAGTGCGAGTAAA
CGAGCTGAAATTGAGGGAGTAGCGAGAGAAATTCTTCATTTAGTGAGAGAAGAAGGACTTAGACTGCGAGATATTACAGT
TGTCGCGCGGCATGTAGATGACTATAAAGATACGTTAAAAGAGGTTTTTCGAGATTACGATATTCCTTTTTTTATTGATG
GAAATGAATCAATGCAGTATCATCCGCTCATTGAATTGATTCGTTCGAGCTTGGATGTCATAAAAGGAAACTGGCGGTAT
GAGGCCGTGTTTCGCTGCGTGAAAACAGAGTTCTTATTTCCGCTCGAACTTGCCAAAAACAAAGCGAGAGAACAGGCAGA
TCAGCTTGAAAACTACTGTATTGCTTATGGTGTAAAAGGAGAACGCTGGACAAACGGCTCCCGTTTCCACTACAGACGAT
TCCAATCTTTAGATGAGGATTTTGGACAAACAGATCAAGAAATTGAAATGGAACAGATGCTGAATGATGTAAAGGAATGG
ATTGCACCTCCGCTTTTCAAATTGCAAAAAAGGCTGAAAAAAGCGCAAAAGGTAAAAGACATGGTGGAGGCAGTCTATGT
CTTTTTAGAGGAAATACAAGTGCCAGATAAACTTGAAAAAGCTAGGCTGGAAGCAGAAGAAGCAGGTAGATTAGCTGAAG
CCATGCAGCATGGACAAGTATGGGATGCTGTTATTCAATTAATGGATGAATTTGTTGAAATGCTCGGTGAGGAGGAGCTC
TCATTTCCTTTATTCCAGCAAATGATGGATACAGGATTGGCCTCTTTAAAATTCGCTTTAATTCCGCCTTCACTCGACCA
AGTATTTATCGGAAGTATGGATTTATCAAGAATGTATCAAGTGAAGTGTATGTTTATCATTGGCGTAAATGATGGCGTTA
TTCCCGCACGCCCTTCTGACGAAAGTGTTTTGTCTGAGGATGACAGGGAATGGTTAAAGCGAGCAGGAGCAGAGCTGGCA
GAGACAGGAAAGGAACGGCTGCTAGATGAGCAATTTTTAATTTACCAGGCGCTGTCGAGTCCATCTCATCATTTGTATCT
GTCTTATTCAGTGTCAGATGAAGAGGGGCGCTCTTTATTGCCTTCAACTCTTATCAAGTATTGTCAGGAGCTTATGCCAA
ATCATCAGCATGCTTTGTATGTGTTAGACCCGGAACAGCTGGATGACGAGGAGCAATTAAAATTTGTAGCGAATGAGCAT
GTCTCGTTATCGTATACTGTCTCGCAGCTGCAGCAATGGCTCAACCAATATCCCATCAGTGGTGTATGGTGGAGTGTTTA
TAATTATTTGATAACGTCAACTCATCGGGATGTATCAAAAAACATCTTGTCGAGTTTGTTTTTTACAAATCGAGCAAAGC
GCTTAAAACCAAACGTCACAAAAGAACTTTACGGTGATCACATTCAAGGCAGTGTTTCAAGAATGGAGAAATTCAATGCG
TGTGCATTTTCTCACTTTGCTTCCCACGGGTTAAAACTAAAGGACCGGCAATTTTATAAATTGGAAGCACCCGATATCGG
CCAGCTGTTTCACTCGGCTTTAAAGCATATTTCAGATACGCTTGTTGAACAAAAAAAGGATTGGAAAAACTTAACGAAGG
ATGACTGTGTCAACTATTCAAGACATGCAATCGAACAGCTTGCTCCGCGTCTTCAAAAGGAAATCCTCTTAAGCTCTAAT
CGGCATGCTTATATTAAAGAAAAACTCCAAAAAATCTTGATTCGGGTCTCTTCTATCTTAAGTGAGCATGCAAGAGTGAG
TGGATTTTCTCCCGTTGGACTTGAGTTAGGGTTTGGCGGCCAAGGACCTCTTCCGCCGTTTACTTTTCAATTGAAAAATG
GCTGCACGATGGAGCTTGTCGGAAGAATTGACCGCGTAGATAAAGCAGAAGGTTCAAAAGGGCTGTTGTTGAGAATCGTC
GACTACAAATCGAGTGAGAAAGGTCTTGATCTAGCAGAGGTATACTATGGGCTGGCCTTGCAAATGCTCACTTACTTAGA
TTTAACCATTACGTATTCGAAGGAATGGCTCGGTGTAGAAGCAACGCCAGCTGGCATTTTATATTTCCATATCCATGATC
CGCTTATTCAAGCACCGATTCCGCTTGCAGAGGATGAAATTGAGCAGGAAATATTCAAGAAATTTAAAATGAAAGGTCTA
TTGCTTGAAGATGTAGAAGCGGTAAAGCTAATGGATCAAACGCTTGAATCAGGAAGATCACAAGTCATTCAAGCCGGTTT
GAAAAAGGACGGATCTTTCCGTTCTGACTCTGCTGTTTTAAGTGAAGATCATTTCCATATACTCACAAAGCACGTTCGGC
GCACATTTGAAGAAGCAGGCGAAAGAATTACAAATGGTGAAATAGCCATTAATCCATATAAATTGAAGGATCAAACACCA
TGCCGCTTTTGTTCATTTAAGTCTATTTGCCAATTTGATGAATCGATAGAGGATAATGAATTTAGAGTGCTATCATCTGA
AAAGGATGATGTTGTGATAGACCGGATCAAAAAAGAAGGGGATCAGTATGCAAATACCAAAACCGAATAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

65.805

99.316

0.654