Detailed information NTG0729209

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: N4558_RS21285
• Genome accession: NZ_CP104373
• Coordinates: 4417958..4418758 (+)
• Length: 266 a.a.
• NCBI ID: WP_019705879.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain 2021CK-01815
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4412958..4423758

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
N4558_RS21250 (N4558_21245)	gspH	4413114..4413629 (+)	516	WP_004151939.1	type II secretion system minor pseudopilin GspH	-
N4558_RS21255 (N4558_21250)	gspI	4413626..4413991 (+)	366	WP_004894160.1	type II secretion system minor pseudopilin GspI	-
N4558_RS21260 (N4558_21255)	gspJ	4413988..4414584 (+)	597	WP_019705881.1	type II secretion system minor pseudopilin GspJ	-
N4558_RS21265 (N4558_21260)	gspK	4414581..4415567 (+)	987	WP_004178610.1	type II secretion system minor pseudopilin GspK	-
N4558_RS21270 (N4558_21265)	gspL	4415554..4416747 (+)	1194	WP_019705880.1	type II secretion system protein GspL	-
N4558_RS21275 (N4558_21270)	-	4416740..4417225 (+)	486	WP_004183037.1	type II secretion system protein M	-
N4558_RS21280 (N4558_21275)	-	4417215..4417961 (+)	747	WP_004145897.1	type II secretion system protein N	-
N4558_RS21285 (N4558_21280)	pilD	4417958..4418758 (+)	801	WP_019705879.1	prepilin peptidase	Machinery gene
N4558_RS21290 (N4558_21285)	hrpB	4418806..4421235 (-)	2430	WP_019705878.1	ATP-dependent helicase HrpB	-
N4558_RS21295 (N4558_21290)	thpR	4421308..4421844 (+)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
N4558_RS21300 (N4558_21295)	sfsA	4421844..4422560 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
N4558_RS21305 (N4558_21300)	dksA	4422723..4423178 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29435.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=729209 N4558_RS21285 WP_019705879.1 4417958..4418758(+) (pilD) [Klebsiella pneumoniae strain 2021CK-01815]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPISWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGSIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=729209 N4558_RS21285 WP_019705879.1 4417958..4418758(+) (pilD) [Klebsiella pneumoniae strain 2021CK-01815]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCTCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGAGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCTTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGAGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425