Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   N4269_RS01290 Genome accession   NZ_CP104356
Coordinates   274243..274833 (+) Length   196 a.a.
NCBI ID   WP_000157688.1    Uniprot ID   A0A655VUM8
Organism   Vibrio cholerae strain PNUSAV001141     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 269243..279833
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N4269_RS01275 (N4269_01275) - 270040..272517 (-) 2478 WP_260813123.1 penicillin-binding protein 1A -
  N4269_RS01280 (N4269_01280) pilM 272660..273676 (+) 1017 WP_000520444.1 type IV pilus assembly protein PilM Machinery gene
  N4269_RS01285 (N4269_01285) pilN 273666..274250 (+) 585 WP_057555158.1 PilN domain-containing protein Machinery gene
  N4269_RS01290 (N4269_01290) pilO 274243..274833 (+) 591 WP_000157688.1 type 4a pilus biogenesis protein PilO Machinery gene
  N4269_RS01295 (N4269_01295) pilP 274823..275341 (+) 519 WP_000792032.1 pilus assembly protein PilP Machinery gene
  N4269_RS01300 (N4269_01300) pilQ 275367..277082 (+) 1716 WP_000788426.1 type IV pilus secretin PilQ Machinery gene
  N4269_RS01305 (N4269_01305) aroK 277269..277793 (+) 525 WP_000818616.1 shikimate kinase AroK -
  N4269_RS01310 (N4269_01310) aroB 277821..278906 (+) 1086 WP_260813124.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 196 a.a.        Molecular weight: 22891.30 Da        Isoelectric Point: 4.4169

>NTDB_id=729038 N4269_RS01290 WP_000157688.1 274243..274833(+) (pilO) [Vibrio cholerae strain PNUSAV001141]
MASLQELELDEISEWPLLPQLLVILLIMLLIQGAGYWFYLMPKQDEIALLKQEEETVKATLRIKANKVAVLPQIQAQLDE
LKERYDFLLRQLPVQKELASMLASVNQLGLDSSLTFTRIDWGERESQEFLYRLPLNIELTGSYHDIGDFSQAIAKLPRII
NFDDVDWQRVSQESSTLHFRVRAYTYQFKQEVDDEK

Nucleotide


Download         Length: 591 bp        

>NTDB_id=729038 N4269_RS01290 WP_000157688.1 274243..274833(+) (pilO) [Vibrio cholerae strain PNUSAV001141]
ATGGCTAGTTTGCAAGAGCTAGAACTGGATGAAATTAGTGAATGGCCGCTGCTGCCTCAGCTGCTGGTGATTCTGCTTAT
CATGCTACTGATCCAAGGTGCTGGTTATTGGTTCTATCTCATGCCCAAACAAGACGAGATTGCGCTGTTAAAGCAGGAAG
AGGAAACCGTCAAGGCCACACTGCGCATCAAAGCCAATAAAGTGGCGGTATTGCCGCAGATCCAAGCTCAATTGGACGAG
CTCAAAGAGCGTTACGATTTTTTACTGCGCCAGCTACCGGTGCAAAAAGAGTTAGCCAGCATGCTGGCGTCAGTCAACCA
GCTTGGGCTCGATAGTTCGCTTACCTTTACGCGGATTGATTGGGGGGAGCGTGAAAGCCAAGAATTTCTTTACCGCTTAC
CGCTCAATATCGAACTGACGGGAAGCTATCACGATATTGGCGATTTTTCGCAAGCGATTGCCAAGTTACCGCGCATCATC
AACTTCGACGATGTGGACTGGCAGCGGGTCAGCCAAGAGAGTAGCACGCTGCACTTTCGAGTTAGAGCCTACACCTACCA
ATTTAAGCAGGAGGTGGATGATGAAAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(50-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A655VUM8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

99.49

100

0.995
  pilO Vibrio campbellii strain DS40M4

71.429

96.429

0.689