Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   N4T36_RS22120 Genome accession   NZ_CP104347
Coordinates   4069570..4070310 (+) Length   246 a.a.
NCBI ID   WP_000076101.1    Uniprot ID   N9LH62
Organism   Acinetobacter baumannii strain 2021CK-01332     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4064570..4075310
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N4T36_RS22105 (N4T36_22105) ponA 4065157..4067712 (-) 2556 WP_000736683.1 penicillin-binding protein PBP1a -
  N4T36_RS22110 (N4T36_22110) pilM 4067874..4068932 (+) 1059 WP_002014066.1 pilus assembly protein PilM Machinery gene
  N4T36_RS22115 (N4T36_22115) pilN 4068932..4069573 (+) 642 WP_000201227.1 PilN domain-containing protein Machinery gene
  N4T36_RS22120 (N4T36_22120) pilO 4069570..4070310 (+) 741 WP_000076101.1 type 4a pilus biogenesis protein PilO Machinery gene
  N4T36_RS22125 (N4T36_22125) pilP 4070321..4070848 (+) 528 WP_000695058.1 pilus assembly protein PilP Machinery gene
  N4T36_RS22130 (N4T36_22130) pilQ 4070911..4073076 (+) 2166 WP_001017038.1 type IV pilus secretin PilQ family protein Machinery gene
  N4T36_RS22135 (N4T36_22135) aroK 4073115..4073711 (+) 597 WP_313902375.1 shikimate kinase AroK -
  N4T36_RS22140 (N4T36_22140) aroB 4073687..4074769 (+) 1083 WP_001194002.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 246 a.a.        Molecular weight: 27819.59 Da        Isoelectric Point: 4.8357

>NTDB_id=728889 N4T36_RS22120 WP_000076101.1 4069570..4070310(+) (pilO) [Acinetobacter baumannii strain 2021CK-01332]
MSQDELQELSLEQLSAKKKKFNLDKFLQQFNTLDMNNYGSWPISVKITCWIFIFFAVLALGYFIVIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQVQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
IAIEATGDYHAFGAFVSSIAALPRIVTMHDFTVDVSPAKDNKSDIPVLNYSIKAKTYRYVGATDTSEQTGKAPAASASTN
TTVQPK

Nucleotide


Download         Length: 741 bp        

>NTDB_id=728889 N4T36_RS22120 WP_000076101.1 4069570..4070310(+) (pilO) [Acinetobacter baumannii strain 2021CK-01332]
ATGAGTCAAGACGAACTTCAAGAATTGTCATTAGAACAATTATCTGCAAAGAAAAAGAAATTTAATCTCGATAAATTCCT
TCAGCAATTTAATACGCTGGATATGAACAACTACGGTAGTTGGCCAATATCTGTAAAAATTACGTGTTGGATTTTTATTT
TCTTTGCTGTGTTAGCTTTGGGGTATTTCATTGTTATTCAACCAAAATTACAAGCTATTGATAATGCTCAAGCTCAGGAA
AGCAATTTATTAAACGAATTTCGTGAAAAAGACTCGAAATTACGTAACCTGCAACAGTATCAGGTACAACTTCAAGAGAT
GCAGGCAAATTTCAACCAGCAACTAGAGCAGTTACCAAAAGAAACTGAGATTCCGAGTTTAGTTGAAGATATCAACTTAA
CAGGAGTGAACTCTGGATTGAAATTTAAAAATATTCGCTTGGAAGATGAAGTAAAGCAGGAAATTTTTATTGAGCAACCA
ATTGCCATAGAAGCGACCGGAGACTATCACGCATTTGGAGCTTTTGTAAGTAGTATCGCTGCTTTGCCTCGTATTGTAAC
CATGCACGATTTTACTGTTGATGTATCGCCAGCTAAGGATAACAAGTCTGATATTCCTGTACTGAACTATTCCATTAAAG
CAAAAACATACCGATACGTAGGGGCAACAGATACATCGGAGCAAACAGGCAAAGCTCCAGCAGCATCTGCTTCAACGAAC
ACTACTGTTCAGCCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9LH62

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

100

100

1
  comO Acinetobacter baylyi ADP1

69.421

98.374

0.683