NTDB

Overview

Name	dprA	Type	Machinery gene
Locus tag	NUE46_RS02480	Genome accession	NZ_CP104309
Coordinates	501030..502154 (+)	Length	374 a.a.
NCBI ID	WP_000228544.1	Uniprot ID	A0A9P2PX49
Organism	Escherichia coli O157:H7 strain 380-94
Function	ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 496030..507154

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NUE46_RS02455 (NUE46_02455)	mscL	496156..496566 (-)	411	WP_000022442.1	large-conductance mechanosensitive channel protein MscL	-
NUE46_RS02460 (NUE46_02460)	trkA	496696..498072 (-)	1377	WP_000691382.1	Trk system potassium transporter TrkA	-
NUE46_RS02465 (NUE46_02465)	rsmB	498094..499383 (-)	1290	WP_001301795.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
NUE46_RS02470 (NUE46_02470)	fmt	499429..500376 (-)	948	WP_000004459.1	methionyl-tRNA formyltransferase	-
NUE46_RS02475 (NUE46_02475)	def	500391..500900 (-)	510	WP_000114984.1	peptide deformylase	-
NUE46_RS02480 (NUE46_02480)	dprA	501030..502154 (+)	1125	WP_000228544.1	DNA-protecting protein DprA	Machinery gene
NUE46_RS02485 (NUE46_02485)	smg	502126..502599 (+)	474	WP_000460672.1	DUF494 family protein Smg	-
NUE46_RS02490 (NUE46_02490)	yrdD	502628..503170 (+)	543	WP_001129722.1	type I DNA topoisomerase	-
NUE46_RS02495 (NUE46_02495)	tsaC	503175..503747 (+)	573	WP_001301437.1	L-threonylcarbamoyladenylate synthase type 1 TsaC	-
NUE46_RS02500 (NUE46_02500)	aroE	503752..504570 (+)	819	WP_000451254.1	shikimate dehydrogenase	-
NUE46_RS02505 (NUE46_02505)	yrdB	504567..504824 (+)	258	WP_001070590.1	DUF1488 domain-containing protein	-
NUE46_RS02510 (NUE46_02510)	yrdA	504800..505354 (-)	555	WP_001286216.1	gamma carbonic anhydrase family protein	-

Sequence

Protein

Download Length: 374 a.a. Molecular weight: 41005.96 Da Isoelectric Point: 6.2655

                                >NTDB_id=728541 NUE46_RS02480 WP_000228544.1 501030..502154(+) (dprA) [Escherichia coli O157:H7 strain 380-94]

MVDTEIWLRLMSISSLYGDDMVRIAHWLARQSHIDAVVLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE

FYPPQLLATTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLAKHGVTITSGLARGIDGVAHKAALQVN

GVSIAVLGNGLNTIHPRRHARLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA

LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDE

VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide

Download Length: 1125 bp

                                >NTDB_id=728541 NUE46_RS02480 WP_000228544.1 501030..502154(+) (dprA) [Escherichia coli O157:H7 strain 380-94]

ATGGTCGATACAGAAATTTGGCTGCGTTTAATGAGTATCAGCAGCTTGTACGGCGATGATATGGTCCGTATAGCTCACTG

GCTGGCAAGACAGTCGCATATTGATGCGGTTGTATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT

CATTTCCGCGGAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATCCCTGCGGACAGCGAA

TTTTATCCTCCTCAACTTCTGGCGACGACAGATTACCCCGGTGCACTGTTTGTTGAAGGAGAACTGCACGCGCTGCATTC

ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGATTATTTTGCGAAACTCTGG

CGAAGCATGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTAGCGCATAAAGCAGCCTTACAGGTAAAT

GGCGTCAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGTCATGCCCGACTGGCTGCCAGTCTGCT

TGAACAGGGGGGCGCTCTCGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA

TTATCAGTGGTCTAAGTAAAGGTGTACTGGTGGTGGAAGCGGCTTTGCGTAGTGGTTCGCTGGTGACAGCACGTTGTGCG

CTTGAGCAGGGGCGAGAAGTTTTTGCCTTGCCAGGTCCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA

ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC

CTGAAAATTCATTTTATTCACCAGATCAGGAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG

GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT

AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG

TTTGA

Domains

Predicted by InterproScan.

DprA winged helix domain (DprA_WH)

(312-357)

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
dprA	Vibrio campbellii strain DS40M4	48.78	98.663	0.481
dprA	Glaesserella parasuis strain SC1401	45.401	90.107	0.409
dprA	Vibrio cholerae strain A1552	49.355	82.888	0.409
dprA	Haemophilus influenzae Rd KW20	44.214	90.107	0.398
dprA	Legionella pneumophila strain ERS1305867	44.545	88.235	0.393

Detailed information NTG0728541