Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   NYP20_RS25145 Genome accession   NZ_CP103965
Coordinates   5719184..5720773 (+) Length   529 a.a.
NCBI ID   WP_259496722.1    Uniprot ID   -
Organism   Pseudomonas sp. N3-W     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5714184..5725773
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYP20_RS25125 (NYP20_25125) pgeF 5715971..5716696 (-) 726 WP_259496716.1 peptidoglycan editing factor PgeF -
  NYP20_RS25130 (NYP20_25130) rluD 5716693..5717655 (-) 963 WP_140681055.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  NYP20_RS25135 (NYP20_25135) - 5717807..5718832 (+) 1026 WP_259496719.1 outer membrane protein assembly factor BamD -
  NYP20_RS25140 (NYP20_25140) - 5718961..5719194 (+) 234 WP_259496720.1 PP0621 family protein -
  NYP20_RS25145 (NYP20_25145) pilS 5719184..5720773 (+) 1590 WP_259496722.1 PAS domain-containing sensor histidine kinase Regulator
  NYP20_RS25150 (NYP20_25150) pilR 5720770..5722113 (+) 1344 WP_259496723.1 sigma-54 dependent transcriptional regulator Regulator
  NYP20_RS25155 (NYP20_25155) thiO 5722234..5723334 (-) 1101 WP_259496724.1 glycine oxidase ThiO -
  NYP20_RS25160 (NYP20_25160) - 5723389..5723790 (-) 402 WP_259496725.1 type IV pilin protein -

Sequence

Protein


Download         Length: 529 a.a.        Molecular weight: 58265.03 Da        Isoelectric Point: 6.7570

>NTDB_id=726681 NYP20_RS25145 WP_259496722.1 5719184..5720773(+) (pilS) [Pseudomonas sp. N3-W]
MIAEATSIGNKKAQRLLRLYHLYRLSIGITLVLLISSNMDNQLLTFANNDLLRSGSWLYLVLNILLVVFLENTRHPAQLF
GLALTDVLLLCGLFYAAGGVASAIGNLLIVSVAIGNTLLRGRIGLLIAAVGALGIVGLSFLLSFSHPVSPNDYLQAGTLG
ALCFAAALLVQGLIRRLEDSEHLAQQRASEVIGLEALNALILQRMRTGILVLDDQRRVQLANHSALTLLGQDTLNGQLID
DYSPLLVERLQLWLNNPTLRPQGLKIAALGLELQPSFIALDQGSHHQTLVFLEDLAQIAQQAQQLKLAALGRLTAGIAHE
IRNPLGAISHAAQLLQESEELNGADRRLTQIIQDHSQRMNRVIENVLQLSRRQQSAPQRLDLHAWLEQFVEEAAEHGGER
QQIHLHISSGHFSTLMDPNQLTQILDNLLRNALRHSALIHHRPEVWLELFIDPDSQLPALEVLDNGPGVTADQQVHLFEP
FFTTSSQGTGLGLYLSRELCESNQARLDFKPRQGGGGFRITFAHGRKQS

Nucleotide


Download         Length: 1590 bp        

>NTDB_id=726681 NYP20_RS25145 WP_259496722.1 5719184..5720773(+) (pilS) [Pseudomonas sp. N3-W]
GTGATCGCTGAGGCCACCAGCATCGGCAATAAAAAGGCCCAGCGACTGCTGCGCCTTTATCACCTCTACCGTTTAAGCAT
CGGCATCACCCTGGTGCTACTGATCTCCAGCAACATGGACAACCAGTTGCTGACGTTCGCCAATAACGACCTGCTGCGCA
GTGGCAGCTGGTTGTACCTGGTACTGAATATCCTGCTGGTGGTGTTCCTGGAGAACACCCGCCACCCGGCGCAGCTATTC
GGCCTGGCCCTGACCGATGTGTTGCTGCTATGCGGCCTGTTCTACGCTGCCGGCGGCGTGGCCAGTGCCATTGGCAACTT
GCTGATCGTGTCGGTAGCCATCGGCAACACGCTACTGCGGGGCAGGATCGGCCTGCTGATTGCAGCAGTCGGGGCCCTCG
GCATCGTCGGTTTGAGTTTCCTGCTCAGTTTCAGTCATCCCGTCAGCCCCAACGACTACTTGCAAGCCGGCACGCTGGGC
GCGCTGTGCTTTGCCGCTGCGTTGCTGGTGCAAGGGTTGATCCGGCGCCTGGAAGACAGCGAACACCTTGCCCAACAGCG
CGCCAGCGAAGTGATCGGCCTCGAAGCCCTCAACGCGCTGATCCTGCAACGCATGCGTACTGGCATTCTGGTGCTGGACG
ATCAGCGACGGGTGCAACTGGCCAACCACAGTGCCCTGACCCTGCTGGGGCAGGACACGTTGAATGGTCAGTTGATCGAT
GACTATTCGCCGCTGCTGGTCGAGCGCCTGCAGTTGTGGCTCAACAACCCGACCTTGCGCCCACAGGGACTGAAGATCGC
CGCCCTTGGCCTGGAACTGCAGCCAAGCTTCATTGCCCTGGACCAGGGCTCGCATCATCAGACGCTGGTATTTCTAGAAG
ACCTGGCGCAAATCGCCCAGCAGGCCCAGCAACTGAAACTTGCCGCCCTTGGCCGCCTGACTGCCGGGATCGCCCACGAG
ATACGTAATCCGCTGGGCGCCATCAGCCATGCTGCGCAGTTGCTGCAAGAGTCCGAGGAACTGAACGGCGCGGACCGCCG
TCTGACCCAGATTATTCAAGATCACTCTCAGCGCATGAATCGGGTTATCGAAAACGTCCTGCAACTGTCCCGCCGCCAGC
AATCCGCGCCGCAGCGACTCGATCTGCACGCATGGCTTGAGCAGTTCGTCGAGGAGGCCGCTGAACATGGCGGCGAACGT
CAGCAGATTCACCTGCATATCAGTTCAGGCCACTTCAGCACCTTGATGGACCCGAATCAGCTGACGCAAATTCTCGACAA
CTTATTGCGCAACGCCTTGCGTCACAGCGCGCTGATTCATCACCGGCCCGAAGTCTGGCTGGAGCTGTTCATCGACCCGG
ACAGCCAGCTACCGGCCCTTGAAGTGCTGGACAACGGCCCCGGCGTGACGGCAGACCAGCAGGTGCATCTGTTCGAACCC
TTCTTCACCACCAGTAGCCAGGGCACTGGCCTTGGGCTTTATCTGTCCCGTGAGCTGTGCGAAAGCAACCAGGCCCGCCT
AGACTTCAAACCACGCCAAGGCGGCGGCGGCTTTCGCATCACCTTTGCTCACGGACGGAAACAAAGTTGA

Domains

Predicted by InterproScan.

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-522)

His Kinase A (phospho-acceptor) domain (HisKA)

(309-374)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

60.566

100

0.607