Detailed information NTG0726433

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: PWR57_RS00270
• Genome accession: NZ_CP119441
• Coordinates: 45817..48015 (-)
• Length: 732 a.a.
• NCBI ID: WP_001300388.1
• Uniprot ID: -
• Organism: Escherichia coli O121 strain 201805972
• Function: DNA puliing through the inner membrane (predicted from homology) DNA binding and uptake

Genomic Context

Location: 40817..53015

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PWR57_RS00250 (PWR57_00250)	hslU	41621..42952 (-)	1332	WP_001293341.1	HslU--HslV peptidase ATPase subunit	-
PWR57_RS00255 (PWR57_00255)	hslV	42962..43492 (-)	531	WP_000208242.1	ATP-dependent protease subunit HslV	-
PWR57_RS00260 (PWR57_00260)	ftsN	43585..44544 (-)	960	WP_073860315.1	cell division protein FtsN	-
PWR57_RS00265 (PWR57_00265)	cytR	44636..45661 (-)	1026	WP_000644904.1	DNA-binding transcriptional regulator CytR	Regulator
PWR57_RS00270 (PWR57_00270)	priA	45817..48015 (-)	2199	WP_001300388.1	primosomal protein N'	Machinery gene
PWR57_RS00275 (PWR57_00275)	rpmE	48218..48430 (+)	213	WP_000710769.1	50S ribosomal protein L31	-
PWR57_RS00280 (PWR57_00280)	rhsC	48590..50092 (+)	1503	Protein_55	rhs element protein RhsC	-
PWR57_RS27075	-	50114..50731 (+)	618	Protein_56	RHS repeat-associated core domain-containing protein	-
PWR57_RS00285 (PWR57_00285)	-	50733..51056 (+)	324	WP_001323627.1	RHS element protein dsORF-f3	-
PWR57_RS00290 (PWR57_00290)	-	51126..51818 (-)	693	Protein_58	IS1 family transposase	-
PWR57_RS00295 (PWR57_00295)	yiiX	51963..52571 (-)	609	WP_000797341.1	YiiX family permuted papain-like enzyme	-

Sequence

Protein

• Length: 732 a.a.
• Molecular weight: 81680.99 Da
• Isoelectric Point: 9.0297

>NTDB_id=726433 PWR57_RS00270 WP_001300388.1 45817..48015(-) (priA) [Escherichia coli O121 strain 201805972]
MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIGVVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRL
LLWAADYYHHPIGDVLFHALPILLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFSAWLLAGVTGSGKTEVYLSVLEN
VLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVLHSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVID
EEHDSSYKQQEGWRYHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVLDLKGQKVQ
AGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDHYYTLHQAQHHLRCHHCDSQRPVPRQCPSCG
STHLVPVGLGTEQLEQTLAPLFPGVPISRIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVD
GALFSADFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAERRMMQLPPWTSHVIVR
AEDHNNQHAPLFLQQLRNLILSSPLADEKLWVLGPVPALAPKRGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRK
VKWVLDVDPIEG

Nucleotide

• Length: 2199 bp

>NTDB_id=726433 PWR57_RS00270 WP_001300388.1 45817..48015(-) (priA) [Escherichia coli O121 strain 201805972]
ATGCCCGTTGCCCACGTTGCCTTGCCCGTTCCGCTTCCTCGTACCTTTGACTATCTGCTGCCAGAAGGCATGACGGTTAA
AGCCGGGTGTCGCGTGCGCGTGCCGTTTGGCAAACAGCAGGAGCGCATCGGGGTTGTGGTGTCAGTTAGCGATGTCAGTG
AGCTGCCGCTTAACGAGCTGAAAGCGGTAGTGGAAGTGCTGGACGTTGAGCCGGTATTTACTCACTCCGTCTGGCGGTTA
CTGCTCTGGGCGGCAGATTACTATCATCATCCGATTGGCGATGTGCTGTTTCATGCCTTGCCGATTTTACTGCGCCAGGG
GCGGCCTGCGGCGAACGCGCCGATGTGGTACTGGTTTGCCACTGAACAAGGCCAGGCGGTGGATCTGAACAGCCTGAAAC
GCTCCCCCAAGCAACAACAGGCGCTGGCGGCGTTACGGCAAGGCAAAATCTGGCGCGACCAGGTCGCCACGCTCGAATTT
AATGATGCCGCGTTGCAGGCGCTACGCAAAAAAGGTCTGTGTGATTTAGCAAGTGAAACACCAGAGTTTAGCGACTGGCG
AACGAACTATGCCGTTTCTGGTGAGCGGTTGCGATTGAATACCGAACAGGCCACCGCCGTTGGCGCAATTCATAGCGCGG
CAGATACTTTTTCTGCCTGGCTGCTGGCGGGCGTTACCGGTTCCGGTAAAACGGAGGTTTATCTCAGCGTACTGGAAAAC
GTGCTCGCTCAGGGCAAACAGGCGCTGGTGATGGTGCCGGAAATCGGCCTGACACCGCAAACTATCGCCCGTTTTCGTGA
ACGTTTTAATGCCCCCGTGGAAGTTCTGCATTCCGGCCTGAACGACAGCGAGCGTCTTTCGGCGTGGCTGAAAGCGAAAA
ATGGTGAGGCGGCGATTGTGATCGGCACCCGCTCCGCGCTGTTTACGCCGTTTAAAAATCTCGGCGTGATTGTCATTGAT
GAAGAGCACGACAGCTCCTACAAGCAGCAGGAAGGCTGGCGCTATCATGCCCGCGACCTGGCGGTGTATCGTGCGCACAG
CGAGCAAATCCCGATTATTCTTGGCTCCGCAACGCCCGCGCTGGAAACGTTATGCAACGTCCAGCAGAAAAAATACCGCC
TGCTGCGCCTGACCCGTCGGGCAGGGAATGCGCGTCCGGCAATTCAACATGTGCTGGATTTAAAAGGTCAGAAGGTGCAG
GCAGGTCTGGCTCCGGCGTTAATCACTCGTATGCGCCAGCATTTACAGGCTGATAACCAGGTCATTCTCTTTCTTAACCG
CCGTGGCTTTGCGCCTGCACTGCTGTGCCACGACTGTGGCTGGATTGCCGAATGCCCACGTTGCGATCACTACTACACGC
TGCATCAGGCGCAGCACCATCTGCGCTGCCACCACTGTGACAGTCAGCGTCCGGTGCCGCGCCAGTGCCCTTCCTGCGGT
TCCACGCACCTGGTCCCCGTGGGGCTGGGCACCGAACAGCTTGAACAGACGCTCGCGCCGTTGTTCCCCGGCGTGCCCAT
TTCTCGTATCGACCGCGATACCACCAGCCGCAAAGGGGCGCTGGAACAGCAACTGGCAGAAGTACATCGCGGCGGCGCGC
GGATTTTGATTGGTACACAAATGCTGGCGAAAGGTCACCATTTCCCGGATGTGACGCTGGTTGCATTACTGGACGTGGAC
GGCGCGCTGTTTTCTGCCGATTTTCGCTCGGCAGAGCGTTTCGCTCAGCTTTACACCCAGGTCGCCGGTCGTGCCGGGCG
TGCGGGTAAACAGGGCGAAGTGGTGCTGCAAACGCACCATCCGGAACATCCTCTGTTGCAAACGTTGCTCTATAAAGGCT
ACGACGCCTTTGCCGAACAGGCGCTGGCTGAGCGGCGAATGATGCAGCTACCGCCGTGGACCAGCCATGTGATTGTGCGT
GCGGAAGATCATAACAATCAGCACGCGCCATTGTTCCTGCAACAACTGCGTAATCTGATCCTCTCCAGCCCACTGGCAGA
CGAGAAACTGTGGGTTCTCGGTCCGGTTCCGGCTCTGGCACCTAAACGTGGCGGTCGCTGGCGCTGGCAGATATTGTTGC
AGCACCCTTCCCGCGTGCGCTTGCAACACATCATTAACGGTACGCTGGCGCTCATCAATACAATACCGGATTCCCGTAAG
GTGAAATGGGTGCTGGATGTTGATCCGATTGAGGGTTAA

Domains

Predicted by InterProScan.

• Primosomal protein N C-terminal domain (PriA_C) (632-729)

• DEAD/DEAH box helicase (DEAD) (203-362)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (445-471)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (6-101)

• Helicase conserved C-terminal domain (Helicase_C) (522-589)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Acinetobacter baumannii D1279779	44.64	100	0.449