Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   NYR63_RS06700 Genome accession   NZ_CP103834
Coordinates   1471393..1471941 (-) Length   182 a.a.
NCBI ID   WP_279456840.1    Uniprot ID   -
Organism   Actinobacillus genomosp. 1 strain 3216     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1466393..1476941
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR63_RS06685 (NYR63_06680) - 1466875..1467396 (+) 522 WP_279456837.1 SprT family zinc-dependent metalloprotease -
  NYR63_RS06690 (NYR63_06685) - 1467501..1468787 (+) 1287 WP_279456838.1 NAD(P)/FAD-dependent oxidoreductase -
  NYR63_RS06695 (NYR63_06690) - 1468979..1471318 (-) 2340 WP_279456839.1 Tex family protein -
  NYR63_RS06700 (NYR63_06695) pilF 1471393..1471941 (-) 549 WP_279456840.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  NYR63_RS06705 (NYR63_06700) - 1471995..1473176 (-) 1182 WP_279456841.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  NYR63_RS06730 (NYR63_06725) gltX 1474084..1475523 (+) 1440 WP_279456842.1 glutamate--tRNA ligase -
  NYR63_RS06740 (NYR63_06735) yefM 1476025..1476276 (+) 252 WP_279456843.1 YoeB-YefM toxin-antitoxin system antitoxin YefM -
  NYR63_RS06745 (NYR63_06740) - 1476273..1476533 (+) 261 WP_279456844.1 Txe/YoeB family addiction module toxin -
  NYR63_RS06750 (NYR63_06745) - 1476691..1476858 (-) 168 WP_279456845.1 Trm112 family protein -

Sequence

Protein


Download         Length: 182 a.a.        Molecular weight: 20635.32 Da        Isoelectric Point: 7.3686

>NTDB_id=725823 NYR63_RS06700 WP_279456840.1 1471393..1471941(-) (pilF) [Actinobacillus genomosp. 1 strain 3216]
MNLAKFLRNLTACMLLSVLFGCSSQLPTSQTTFNRSEAVKARINLALAYLDQHDLPKAKENIDKALSHDQNDYLPHSVLA
YYYQQIGDQAHAEQAYHKALKLSDNRPDVLNNYGTFLCKQAKFDQAYQQFEQAVKSEQPYYHQADSLENIVLCAKQEPNL
AKANEALNQLEKLDKARAAALR

Nucleotide


Download         Length: 549 bp        

>NTDB_id=725823 NYR63_RS06700 WP_279456840.1 1471393..1471941(-) (pilF) [Actinobacillus genomosp. 1 strain 3216]
ATGAACCTTGCAAAATTTTTGCGAAATTTGACCGCTTGTATGCTGTTATCTGTGCTGTTCGGTTGTTCTTCTCAGCTTCC
AACCTCCCAAACTACCTTTAATCGTTCGGAAGCGGTTAAAGCGCGTATCAATTTAGCCTTAGCGTATCTCGATCAACATG
ATTTACCCAAAGCGAAAGAGAATATTGATAAAGCGTTAAGCCACGATCAAAACGATTACTTACCTCATTCTGTTTTAGCT
TATTACTATCAGCAAATCGGTGATCAAGCTCATGCGGAACAGGCTTATCATAAAGCATTGAAATTAAGTGATAATCGTCC
GGACGTATTAAATAATTACGGTACTTTTCTGTGTAAACAAGCCAAATTTGATCAGGCATATCAGCAGTTTGAGCAAGCGG
TGAAAAGTGAGCAGCCTTACTATCATCAAGCCGATAGCCTAGAAAATATTGTGTTATGTGCTAAGCAAGAGCCAAACTTA
GCTAAAGCAAATGAAGCGTTAAACCAATTAGAAAAGCTGGATAAAGCAAGAGCGGCGGCACTGCGCTAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(79-104)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

64.894

100

0.67
  pilF/pilF2 Haemophilus influenzae Rd KW20

46.784

93.956

0.44