Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   NYR86_RS00340 Genome accession   NZ_CP103830
Coordinates   63367..63915 (+) Length   182 a.a.
NCBI ID   WP_279623339.1    Uniprot ID   -
Organism   Actinobacillus equuli subsp. equuli strain 2499     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 58367..68915
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR86_RS00295 (NYR86_00295) lpxK 58372..59352 (+) 981 WP_279481142.1 tetraacyldisaccharide 4'-kinase -
  NYR86_RS00300 (NYR86_00300) - 59354..59521 (+) 168 WP_039195964.1 Trm112 family protein -
  NYR86_RS00310 (NYR86_00310) gltX 59784..61223 (-) 1440 WP_279623338.1 glutamate--tRNA ligase -
  NYR86_RS00335 (NYR86_00335) - 62132..63313 (+) 1182 WP_279437279.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  NYR86_RS00340 (NYR86_00340) pilF 63367..63915 (+) 549 WP_279623339.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  NYR86_RS00345 (NYR86_00345) - 63991..66330 (+) 2340 WP_279623340.1 Tex family protein -
  NYR86_RS00350 (NYR86_00350) - 66524..67804 (-) 1281 WP_279471384.1 NAD(P)/FAD-dependent oxidoreductase -
  NYR86_RS00355 (NYR86_00355) - 67916..68434 (-) 519 WP_279623341.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 182 a.a.        Molecular weight: 20668.35 Da        Isoelectric Point: 6.9481

>NTDB_id=725740 NYR86_RS00340 WP_279623339.1 63367..63915(+) (pilF) [Actinobacillus equuli subsp. equuli strain 2499]
MNLAKFLRNLTACMLLSVLFGCSSQLPTSQTTFNRSEAVKARINLALAYLEQHDLPKAKENIDKALSHDQNDYLPHSVLA
YYYQQIGDQAHAEQAYQQALKLSDNRPDVLNNYGTFLCKQAKFDQAYQQFEQAVKSEQPYYHQADSLENIVLCAKQEPNL
AKVNEALNQLEKLDKARAAALR

Nucleotide


Download         Length: 549 bp        

>NTDB_id=725740 NYR86_RS00340 WP_279623339.1 63367..63915(+) (pilF) [Actinobacillus equuli subsp. equuli strain 2499]
ATGAACCTTGCAAAATTTTTGCGAAATTTGACCGCTTGTATGCTGTTATCTGTGCTGTTCGGTTGTTCTTCTCAGCTTCC
TACCTCTCAAACCACTTTTAATCGCTCGGAAGCGGTTAAAGCCCGTATCAATTTAGCCTTAGCGTATCTCGAGCAACATG
ATTTACCCAAAGCGAAAGAGAATATTGATAAAGCGCTAAGCCACGATCAAAACGATTACTTACCTCACTCCGTTTTAGCT
TATTATTATCAACAAATCGGTGATCAAGCTCACGCCGAGCAAGCTTATCAGCAAGCATTGAAATTAAGTGATAATCGTCC
GGATGTATTAAATAATTACGGTACTTTTCTGTGTAAACAAGCCAAGTTTGATCAGGCATATCAGCAGTTTGAGCAAGCGG
TGAAAAGCGAGCAGCCTTACTATCATCAAGCGGATAGCCTCGAAAATATTGTGTTATGTGCCAAACAAGAGCCGAATTTA
GCTAAAGTAAACGAAGCGTTAAACCAATTAGAAAAGCTGGATAAAGCAAGAGCGGCGGCACTGCGTTAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(79-104)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

66.489

100

0.687
  pilF/pilF2 Haemophilus influenzae Rd KW20

46.784

93.956

0.44