Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   NYR83_RS00395 Genome accession   NZ_CP103828
Coordinates   73917..74465 (+) Length   182 a.a.
NCBI ID   WP_279443309.1    Uniprot ID   -
Organism   Actinobacillus equuli subsp. equuli strain 3009     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 68917..79465
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR83_RS00365 (NYR83_00365) gltX 70330..71769 (-) 1440 WP_279443308.1 glutamate--tRNA ligase -
  NYR83_RS00390 (NYR83_00390) - 72681..73862 (+) 1182 WP_279437279.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  NYR83_RS00395 (NYR83_00395) pilF 73917..74465 (+) 549 WP_279443309.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  NYR83_RS00400 (NYR83_00400) - 74541..76880 (+) 2340 WP_279443310.1 Tex family protein -
  NYR83_RS00405 (NYR83_00405) - 77075..78355 (-) 1281 WP_279443793.1 NAD(P)/FAD-dependent oxidoreductase -
  NYR83_RS00410 (NYR83_00410) - 78467..78985 (-) 519 WP_278225217.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 182 a.a.        Molecular weight: 20668.35 Da        Isoelectric Point: 6.9479

>NTDB_id=725688 NYR83_RS00395 WP_279443309.1 73917..74465(+) (pilF) [Actinobacillus equuli subsp. equuli strain 3009]
MNLAKFLRNLTACMLLSVLFGCSSQLPTSQTTFNRSEAVKARINLALAYLDQHDLPKAKENIDKALSHDQNDYLPHSVLA
YYYQQIGDQAHAEQAYQQALKLSDNRPDVLNNYGTFLCKQAKFDQAYQQFEQAVKSEQPYYHQADSLENIVLCAKQEPNV
VKVNEALNQLEKLDKARAAALR

Nucleotide


Download         Length: 549 bp        

>NTDB_id=725688 NYR83_RS00395 WP_279443309.1 73917..74465(+) (pilF) [Actinobacillus equuli subsp. equuli strain 3009]
ATGAACCTTGCAAAATTTTTGCGAAATTTGACCGCTTGTATGCTGTTATCTGTGCTGTTCGGTTGTTCTTCTCAGCTTCC
TACCTCTCAAACCACTTTTAATCGCTCGGAAGCGGTTAAAGCGCGTATCAATTTAGCCTTAGCGTATCTCGATCAACATG
ATTTACCCAAAGCGAAAGAGAATATTGATAAAGCGCTAAGCCACGATCAAAACGATTACTTACCTCACTCCGTTTTAGCT
TATTACTATCAACAAATCGGTGATCAAGCTCACGCCGAGCAAGCTTATCAGCAAGCATTGAAATTAAGTGATAATCGTCC
GGATGTCTTAAATAATTACGGTACTTTTCTGTGTAAACAAGCCAAGTTTGATCAGGCATATCAGCAGTTTGAGCAAGCGG
TGAAAAGCGAGCAACCTTACTATCATCAAGCCGATAGCCTCGAAAATATTGTGTTATGTGCTAAACAAGAGCCGAATGTC
GTTAAAGTAAACGAAGCATTAAACCAATTAGAAAAGCTGGATAAAGCAAGAGCAGCGGCACTGCGCTAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(79-104)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

65.957

100

0.681
  pilF/pilF2 Haemophilus influenzae Rd KW20

46.784

93.956

0.44