Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   NYR82_RS00335 Genome accession   NZ_CP103827
Coordinates   63950..64498 (+) Length   182 a.a.
NCBI ID   WP_279442117.1    Uniprot ID   -
Organism   Actinobacillus equuli subsp. haemolyticus strain 3090     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 58950..69498
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR82_RS00290 (NYR82_00290) lpxK 58957..59937 (+) 981 WP_279442116.1 tetraacyldisaccharide 4'-kinase -
  NYR82_RS00295 (NYR82_00295) - 59939..60106 (+) 168 WP_039195964.1 Trm112 family protein -
  NYR82_RS00305 (NYR82_00305) gltX 60369..61808 (-) 1440 WP_279438613.1 glutamate--tRNA ligase -
  NYR82_RS00330 (NYR82_00330) - 62715..63896 (+) 1182 WP_278225213.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  NYR82_RS00335 (NYR82_00335) pilF 63950..64498 (+) 549 WP_279442117.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  NYR82_RS00340 (NYR82_00340) - 64574..66913 (+) 2340 WP_279442118.1 Tex family protein -
  NYR82_RS00345 (NYR82_00345) - 67114..69222 (+) 2109 WP_279442119.1 ATP-dependent endonuclease -

Sequence

Protein


Download         Length: 182 a.a.        Molecular weight: 20657.32 Da        Isoelectric Point: 6.9479

>NTDB_id=725665 NYR82_RS00335 WP_279442117.1 63950..64498(+) (pilF) [Actinobacillus equuli subsp. haemolyticus strain 3090]
MNLAKFLRNLTACMLLSVLFGCSSQLPTSQTTFNRSEAVKARINLALAYLDQHDLPKAKENIDKALSHDQNDYLPHSVLA
YYYQQIGDQAHAEQAYQQALKLSDNRPDVLNNYGTFLCKQAKFDQAYQQFEQAVKSEQPYYHQADSLENIVLCAKQEPNL
AKVNEALSQLEKSDKVRAAVLR

Nucleotide


Download         Length: 549 bp        

>NTDB_id=725665 NYR82_RS00335 WP_279442117.1 63950..64498(+) (pilF) [Actinobacillus equuli subsp. haemolyticus strain 3090]
ATGAACCTTGCAAAATTTTTGCGAAATTTGACCGCTTGTATGCTGTTATCTGTATTGTTCGGTTGTTCTTCTCAGCTTCC
TACCTCTCAAACCACTTTTAATCGCTCGGAAGCGGTTAAAGCCCGTATCAATTTAGCCTTAGCGTATCTCGATCAACATG
ATTTACCCAAAGCGAAAGAGAATATTGATAAAGCGCTAAGCCACGATCAAAACGATTACTTACCTCATTCTGTTTTAGCT
TATTACTATCAGCAAATCGGTGATCAAGCTCACGCCGAGCAAGCTTATCAGCAAGCATTGAAATTAAGTGATAATCGTCC
GGATGTCTTAAATAATTACGGTACTTTTCTGTGTAAACAAGCCAAGTTTGATCAGGCATATCAACAGTTTGAGCAAGCGG
TGAAAAGCGAGCAGCCTTACTATCATCAAGCCGATAGCCTCGAAAATATTGTGTTATGTGCCAAACAAGAGCCGAATTTA
GCTAAAGTAAACGAAGCATTAAGCCAATTAGAAAAGTCGGATAAAGTAAGAGCGGCGGTACTGCGCTAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(79-104)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

66.489

100

0.687
  pilF/pilF2 Haemophilus influenzae Rd KW20

46.784

93.956

0.44