Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   NYR76_RS00335 Genome accession   NZ_CP103821
Coordinates   63789..64337 (+) Length   182 a.a.
NCBI ID   WP_279478345.1    Uniprot ID   -
Organism   Actinobacillus equuli subsp. equuli strain 3750     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 58789..69337
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR76_RS00290 (NYR76_00290) lpxK 58793..59773 (+) 981 WP_279478343.1 tetraacyldisaccharide 4'-kinase -
  NYR76_RS00295 (NYR76_00295) - 59775..59942 (+) 168 WP_278225209.1 Trm112 family protein -
  NYR76_RS00305 (NYR76_00305) gltX 60205..61644 (-) 1440 WP_279478344.1 glutamate--tRNA ligase -
  NYR76_RS00330 (NYR76_00330) - 62553..63734 (+) 1182 WP_279437279.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  NYR76_RS00335 (NYR76_00335) pilF 63789..64337 (+) 549 WP_279478345.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  NYR76_RS00340 (NYR76_00340) - 64413..66752 (+) 2340 WP_279478346.1 Tex family protein -
  NYR76_RS00345 (NYR76_00345) - 66945..68225 (-) 1281 WP_279441424.1 NAD(P)/FAD-dependent oxidoreductase -
  NYR76_RS00350 (NYR76_00350) - 68337..68855 (-) 519 WP_179130574.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 182 a.a.        Molecular weight: 20727.42 Da        Isoelectric Point: 6.9481

>NTDB_id=725535 NYR76_RS00335 WP_279478345.1 63789..64337(+) (pilF) [Actinobacillus equuli subsp. equuli strain 3750]
MNLAKFLRNLTACMLLSVLFGCSSQIPTSQTTFNRSEAVKARINLALAYLEQHDLPKAKENIDKALSHDQNDYLPHSVLA
YYYQQIGDQAHAEQAYQQALKLSDNRPDVLNNYGTFLCKQAKFDQAYQQFEQAVKSEQPYYHQADSLENIVLCAKQEQNL
VKVNEALNQLEKLDKARAAALR

Nucleotide


Download         Length: 549 bp        

>NTDB_id=725535 NYR76_RS00335 WP_279478345.1 63789..64337(+) (pilF) [Actinobacillus equuli subsp. equuli strain 3750]
ATGAACCTTGCAAAATTTTTGCGAAATTTGACCGCTTGTATGCTGTTATCTGTGCTGTTCGGTTGTTCTTCTCAGATTCC
TACCTCTCAAACCACTTTTAATCGCTCGGAAGCGGTTAAAGCCCGTATCAATTTAGCCTTAGCGTATCTCGAGCAACATG
ATTTACCCAAAGCGAAAGAGAATATTGATAAAGCGCTAAGCCACGATCAAAACGATTACTTACCTCATTCCGTTTTAGCT
TATTATTATCAGCAAATCGGTGATCAAGCTCACGCCGAACAAGCGTATCAGCAAGCATTGAAATTAAGTGATAATCGTCC
GGATGTATTAAATAATTACGGTACTTTTCTGTGTAAACAAGCCAAGTTTGATCAGGCATATCAGCAGTTTGAGCAAGCGG
TGAAAAGCGAGCAGCCTTACTATCATCAAGCCGATAGCCTCGAAAATATAGTGCTATGTGCCAAACAAGAGCAGAATTTG
GTTAAAGTAAACGAAGCGTTAAACCAATTAGAAAAGCTGGATAAAGCAAGAGCAGCGGCACTGCGTTAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(79-104)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

66.489

100

0.687
  pilF/pilF2 Haemophilus influenzae Rd KW20

47.368

93.956

0.445