Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   NYR75_RS00340 Genome accession   NZ_CP103820
Coordinates   64640..65188 (+) Length   182 a.a.
NCBI ID   WP_279437280.1    Uniprot ID   -
Organism   Actinobacillus equuli subsp. haemolyticus strain 3820     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 59640..70188
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR75_RS00295 (NYR75_00295) lpxK 59646..60626 (+) 981 WP_279471876.1 tetraacyldisaccharide 4'-kinase -
  NYR75_RS00300 (NYR75_00300) - 60628..60795 (+) 168 WP_039195964.1 Trm112 family protein -
  NYR75_RS00310 (NYR75_00310) gltX 61058..62497 (-) 1440 WP_279471877.1 glutamate--tRNA ligase -
  NYR75_RS00335 (NYR75_00335) - 63405..64586 (+) 1182 WP_278225213.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  NYR75_RS00340 (NYR75_00340) pilF 64640..65188 (+) 549 WP_279437280.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  NYR75_RS00345 (NYR75_00345) - 65264..67603 (+) 2340 WP_279471878.1 Tex family protein -
  NYR75_RS00350 (NYR75_00350) - 67804..69912 (+) 2109 WP_279442119.1 ATP-dependent endonuclease -

Sequence

Protein


Download         Length: 182 a.a.        Molecular weight: 20683.40 Da        Isoelectric Point: 6.9479

>NTDB_id=725507 NYR75_RS00340 WP_279437280.1 64640..65188(+) (pilF) [Actinobacillus equuli subsp. haemolyticus strain 3820]
MNLAKFLRNLTACMLLSVLFGCSSQLPTSQTTFNRSEAVKARINLALAYLDQHDLPKAKENIDKALSHDQNDYLPHSVLA
YYYQQIGDQAHAEQAYQQALKLSDNRPDVLNNYGTFLCKQAKFDQAYQQFEQAVKSEQPYYHQADSLENIVLCAKQEPNL
AKVNEALSQLEKLDKVRAAVLR

Nucleotide


Download         Length: 549 bp        

>NTDB_id=725507 NYR75_RS00340 WP_279437280.1 64640..65188(+) (pilF) [Actinobacillus equuli subsp. haemolyticus strain 3820]
ATGAACCTTGCAAAATTTTTGCGAAATTTGACCGCTTGTATGTTGTTATCTGTGCTGTTCGGTTGTTCTTCTCAGCTTCC
TACCTCTCAAACCACTTTTAATCGCTCGGAAGCGGTTAAAGCGCGTATCAATTTAGCCTTAGCGTATCTCGATCAACATG
ATTTACCCAAAGCGAAAGAGAATATTGATAAAGCGCTAAGCCACGATCAAAACGATTACTTACCTCATTCTGTTTTAGCT
TATTACTATCAGCAAATCGGTGATCAAGCTCACGCTGAGCAAGCTTATCAGCAAGCATTGAAATTAAGTGATAATCGTCC
GGACGTATTAAATAATTACGGTACTTTTCTGTGTAAACAAGCCAAATTTGATCAGGCATATCAGCAGTTTGAGCAAGCGG
TGAAAAGCGAGCAGCCTTACTATCATCAAGCGGATAGCTTGGAAAATATCGTGTTGTGTGCCAAACAAGAGCCGAATTTA
GCTAAAGTAAACGAAGCATTAAGCCAATTAGAAAAGCTGGATAAAGTAAGAGCGGCGGTACTGCGCTAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(79-104)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

66.489

100

0.687
  pilF/pilF2 Haemophilus influenzae Rd KW20

46.784

93.956

0.44