Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   NYR73_RS00350 Genome accession   NZ_CP103818
Coordinates   64957..65505 (+) Length   182 a.a.
NCBI ID   WP_279453518.1    Uniprot ID   -
Organism   Actinobacillus equuli subsp. haemolyticus strain 3874     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 59957..70505
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR73_RS00305 (NYR73_00305) - 60813..60980 (+) 168 WP_039195964.1 Trm112 family protein -
  NYR73_RS00315 (NYR73_00315) gltX 61243..62683 (-) 1441 Protein_59 glutamate--tRNA ligase -
  NYR73_RS00345 (NYR73_00345) - 63722..64903 (+) 1182 WP_279437279.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  NYR73_RS00350 (NYR73_00350) pilF 64957..65505 (+) 549 WP_279453518.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  NYR73_RS00355 (NYR73_00355) - 65581..67920 (+) 2340 WP_279453519.1 Tex family protein -
  NYR73_RS00360 (NYR73_00360) - 68121..70229 (+) 2109 WP_279453520.1 ATP-dependent endonuclease -

Sequence

Protein


Download         Length: 182 a.a.        Molecular weight: 20688.47 Da        Isoelectric Point: 6.6304

>NTDB_id=725461 NYR73_RS00350 WP_279453518.1 64957..65505(+) (pilF) [Actinobacillus equuli subsp. haemolyticus strain 3874]
MNLAKFLRNLTACMLLSVLFGCSSLLPPPQTTFNRSEAVKARINLALAYLEQHDLPKAKENIDKALSHDQNDYLPHSVLA
YYYQQIGDQAHAEQAYQQALKLSDNRPDVLNNYGTFLCKQAKFDQAYQQFEQAVQSEQPYYHQADSLENIVLCAKQEPNL
AKVNEALSQLEKLDKVRAAVLR

Nucleotide


Download         Length: 549 bp        

>NTDB_id=725461 NYR73_RS00350 WP_279453518.1 64957..65505(+) (pilF) [Actinobacillus equuli subsp. haemolyticus strain 3874]
ATGAACCTTGCAAAATTTTTGCGAAATTTGACCGCTTGTATGCTGTTATCTGTGTTGTTCGGTTGTTCTTCTCTGCTTCC
TCCCCCCCAAACCACTTTTAATCGTTCAGAAGCAGTTAAAGCGCGTATCAATTTAGCCTTAGCGTATCTCGAGCAACATG
ATTTACCCAAAGCGAAAGAGAATATTGATAAAGCGCTAAGCCACGATCAAAACGATTACTTACCTCACTCCGTTTTAGCT
TATTATTATCAACAAATCGGTGATCAAGCTCACGCCGAGCAAGCTTATCAGCAAGCATTGAAATTAAGTGATAATCGTCC
GGATGTATTAAATAATTACGGTACTTTTCTGTGTAAACAAGCTAAGTTTGATCAGGCATATCAGCAGTTTGAGCAAGCGG
TGCAAAGCGAGCAGCCTTACTATCATCAAGCCGATAGCCTCGAAAATATTGTGTTATGTGCCAAACAAGAGCCGAATTTA
GCTAAAGTAAACGAAGCATTAAGCCAATTAGAAAAGCTGGATAAAGTAAGAGCGGCGGTACTGCGCTAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(79-104)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

65.957

100

0.681
  pilF/pilF2 Haemophilus influenzae Rd KW20

45.614

93.956

0.429