Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   NYR71_RS00340 Genome accession   NZ_CP103817
Coordinates   63549..64097 (+) Length   182 a.a.
NCBI ID   WP_279439738.1    Uniprot ID   -
Organism   Actinobacillus equuli subsp. equuli strain 4005     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 58549..69097
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR71_RS00295 (NYR71_00295) lpxK 58550..59530 (+) 981 WP_279439737.1 tetraacyldisaccharide 4'-kinase -
  NYR71_RS00300 (NYR71_00300) - 59532..59699 (+) 168 WP_039195964.1 Trm112 family protein -
  NYR71_RS00310 (NYR71_00310) gltX 59962..61401 (-) 1440 WP_278225211.1 glutamate--tRNA ligase -
  NYR71_RS00335 (NYR71_00335) - 62313..63494 (+) 1182 WP_278225213.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  NYR71_RS00340 (NYR71_00340) pilF 63549..64097 (+) 549 WP_279439738.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  NYR71_RS00345 (NYR71_00345) - 64173..66512 (+) 2340 WP_279439739.1 Tex family protein -
  NYR71_RS00350 (NYR71_00350) - 66706..67986 (-) 1281 WP_279440271.1 NAD(P)/FAD-dependent oxidoreductase -
  NYR71_RS00355 (NYR71_00355) - 68098..68616 (-) 519 WP_179130574.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 182 a.a.        Molecular weight: 20696.36 Da        Isoelectric Point: 6.6304

>NTDB_id=725434 NYR71_RS00340 WP_279439738.1 63549..64097(+) (pilF) [Actinobacillus equuli subsp. equuli strain 4005]
MNLAKFLRNLTACMLLSVLFGCSSQIPTSQTTFNRSEAVKARINLALAYLEQHDLPKAKENIDKALSHDQNDYLPHSVLA
YYYQQIGDQAHAEQAYQQALKLSDNRPDVLNNYGTFLCKQAKFDQAYQQFEQAVQSEQPYYHQADSLENIVLCAKQEPNL
VKVNEALNQLEKLDKARAAALR

Nucleotide


Download         Length: 549 bp        

>NTDB_id=725434 NYR71_RS00340 WP_279439738.1 63549..64097(+) (pilF) [Actinobacillus equuli subsp. equuli strain 4005]
ATGAACCTTGCAAAATTTTTGCGAAATTTGACCGCTTGTATGCTGTTATCTGTGCTGTTCGGTTGTTCTTCTCAGATTCC
TACCTCTCAAACCACTTTTAATCGCTCGGAAGCGGTTAAAGCCCGTATCAATTTAGCCTTAGCGTATCTCGAGCAACATG
ATTTACCCAAAGCGAAAGAGAATATTGATAAAGCGCTAAGCCACGATCAAAACGATTACTTACCTCACTCCGTTTTAGCT
TATTATTATCAACAAATCGGTGATCAAGCTCACGCCGAGCAAGCTTATCAGCAAGCATTGAAATTAAGTGATAATCGTCC
GGATGTATTAAATAATTACGGTACTTTTCTGTGTAAACAAGCCAAGTTTGATCAGGCATATCAACAGTTTGAGCAAGCGG
TGCAAAGCGAGCAGCCTTACTATCATCAAGCCGATAGCCTCGAAAATATAGTGCTATGTGCCAAACAAGAGCCGAATTTG
GTTAAAGTAAACGAAGCGTTAAACCAATTAGAAAAGCTGGATAAAGCAAGAGCAGCGGCACTGCGTTAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(79-104)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

66.489

100

0.687
  pilF/pilF2 Haemophilus influenzae Rd KW20

46.784

93.956

0.44