Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   NYR70_RS00340 Genome accession   NZ_CP103816
Coordinates   63266..63814 (+) Length   182 a.a.
NCBI ID   WP_279477291.1    Uniprot ID   -
Organism   Actinobacillus equuli subsp. equuli strain 4026     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 58266..68814
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR70_RS00295 (NYR70_00295) lpxK 58271..59251 (+) 981 WP_279477289.1 tetraacyldisaccharide 4'-kinase -
  NYR70_RS00300 (NYR70_00300) - 59253..59420 (+) 168 WP_039195964.1 Trm112 family protein -
  NYR70_RS00310 (NYR70_00310) gltX 59683..61122 (-) 1440 WP_279477290.1 glutamate--tRNA ligase -
  NYR70_RS00335 (NYR70_00335) - 62030..63211 (+) 1182 WP_279437279.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  NYR70_RS00340 (NYR70_00340) pilF 63266..63814 (+) 549 WP_279477291.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  NYR70_RS00345 (NYR70_00345) - 63890..66229 (+) 2340 WP_279477292.1 Tex family protein -
  NYR70_RS00350 (NYR70_00350) - 66423..67703 (-) 1281 WP_279471384.1 NAD(P)/FAD-dependent oxidoreductase -
  NYR70_RS00355 (NYR70_00355) - 67815..68333 (-) 519 WP_279470947.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 182 a.a.        Molecular weight: 20710.43 Da        Isoelectric Point: 6.9479

>NTDB_id=725407 NYR70_RS00340 WP_279477291.1 63266..63814(+) (pilF) [Actinobacillus equuli subsp. equuli strain 4026]
MNLAKFLRNLTACMLLSVLFGCSSQIPTSQTTFNRSEAVKARINLALVYLDQHDLPKAKENIDKALSHDQNDYLPHSVLA
YYYQQIGDQAHAEQAYQQALKLSDNRPDVLNNYGTFLCKQAKFDQAYQQFEQAVKSEQPYYHQADSLENIVLCAKQEPNL
VKVNEALNQLEKLDKARAAALR

Nucleotide


Download         Length: 549 bp        

>NTDB_id=725407 NYR70_RS00340 WP_279477291.1 63266..63814(+) (pilF) [Actinobacillus equuli subsp. equuli strain 4026]
ATGAACCTTGCAAAATTTTTGCGAAATTTGACCGCTTGTATGCTGTTATCTGTGCTGTTCGGTTGTTCTTCTCAGATTCC
TACCTCTCAAACCACTTTTAATCGCTCGGAAGCGGTTAAAGCCCGTATCAATTTAGCCTTAGTGTATCTCGATCAACATG
ATTTACCCAAAGCGAAAGAGAATATTGATAAAGCGCTAAGCCACGATCAAAACGATTACTTACCTCATTCTGTTTTAGCT
TATTACTATCAGCAAATCGGTGATCAAGCTCACGCTGAGCAAGCTTATCAGCAAGCATTGAAATTAAGTGATAATCGTCC
GGACGTATTAAATAATTACGGTACTTTTCTGTGTAAACAAGCCAAGTTTGATCAGGCATATCAGCAGTTTGAGCAAGCGG
TGAAAAGCGAGCAGCCTTACTATCATCAAGCCGATAGCCTCGAAAATATAGTGCTATGTGCCAAACAAGAGCCGAATTTG
GTTAAAGTAAACGAAGCGTTAAACCAATTAGAAAAGCTGGATAAAGCAAGAGCAGCGGCACTGCGTTAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(79-104)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

65.426

100

0.676
  pilF/pilF2 Haemophilus influenzae Rd KW20

46.784

93.956

0.44