Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   NYR68_RS00400 Genome accession   NZ_CP103814
Coordinates   75607..76155 (+) Length   182 a.a.
NCBI ID   WP_279622380.1    Uniprot ID   -
Organism   Actinobacillus equuli subsp. haemolyticus strain 4091     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 70607..81155
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR68_RS00370 (NYR68_00370) gltX 72025..73464 (-) 1440 WP_279438613.1 glutamate--tRNA ligase -
  NYR68_RS00395 (NYR68_00395) - 74372..75553 (+) 1182 WP_278225213.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  NYR68_RS00400 (NYR68_00400) pilF 75607..76155 (+) 549 WP_279622380.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  NYR68_RS00405 (NYR68_00405) - 76230..78569 (+) 2340 WP_279622381.1 Tex family protein -
  NYR68_RS00410 (NYR68_00410) - 78763..80043 (-) 1281 WP_279622830.1 NAD(P)/FAD-dependent oxidoreductase -
  NYR68_RS00415 (NYR68_00415) - 80155..80673 (-) 519 WP_279622382.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 182 a.a.        Molecular weight: 20627.30 Da        Isoelectric Point: 6.9479

>NTDB_id=725353 NYR68_RS00400 WP_279622380.1 75607..76155(+) (pilF) [Actinobacillus equuli subsp. haemolyticus strain 4091]
MNLAKFLRNLTACMLLSVLFGCSSQLPTSQTTFNRSEAVKARINLALAYLDQHDLPKAKENIDKALSHDQNDYLPHSVLA
YYYQQIGDQAHAEQAYQQALKLSDNRPDVLNNYGTFLCKQAKFDQAYQQFEQAVKSEQPYYHQADSLENIVLCAKQEPNL
AKVNEALSQLEKLDKARAAALR

Nucleotide


Download         Length: 549 bp        

>NTDB_id=725353 NYR68_RS00400 WP_279622380.1 75607..76155(+) (pilF) [Actinobacillus equuli subsp. haemolyticus strain 4091]
ATGAACCTTGCAAAATTTTTGCGAAATTTGACCGCTTGTATGCTGTTATCTGTGCTGTTCGGTTGTTCTTCTCAGCTTCC
TACCTCTCAAACCACTTTTAATCGCTCGGAAGCGGTTAAAGCGCGTATCAATTTAGCCTTAGCGTATCTCGATCAACATG
ATTTACCCAAAGCGAAAGAGAATATTGATAAAGCGCTAAGCCACGATCAAAACGATTACTTACCTCATTCTGTTTTAGCT
TATTACTATCAGCAAATCGGTGATCAAGCTCACGCCGAGCAAGCTTATCAGCAAGCATTGAAATTAAGTGATAATCGTCC
GGACGTATTAAATAATTACGGTACTTTTCTGTGTAAACAAGCCAAATTTGATCAGGCATATCAGCAGTTTGAGCAAGCGG
TGAAAAGCGAGCAGCCTTACTATCATCAAGCGGATAGCTTGGAAAATATCGTGTTGTGTGCCAAACAAGAGCCGAATTTA
GCTAAAGTAAACGAAGCGTTAAGCCAATTAGAAAAGCTGGATAAAGCAAGAGCAGCGGCTCTGCGCTAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(79-104)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

66.489

100

0.687
  pilF/pilF2 Haemophilus influenzae Rd KW20

46.784

93.956

0.44