Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   NYR67_RS00340 Genome accession   NZ_CP103813
Coordinates   63754..64302 (+) Length   182 a.a.
NCBI ID   WP_279470945.1    Uniprot ID   -
Organism   Actinobacillus equuli subsp. equuli strain 4130     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 58754..69302
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR67_RS00295 (NYR67_00295) lpxK 58755..59735 (+) 981 WP_279470944.1 tetraacyldisaccharide 4'-kinase -
  NYR67_RS00300 (NYR67_00300) - 59737..59904 (+) 168 WP_039195964.1 Trm112 family protein -
  NYR67_RS00310 (NYR67_00310) gltX 60167..61606 (-) 1440 WP_279438613.1 glutamate--tRNA ligase -
  NYR67_RS00335 (NYR67_00335) - 62518..63699 (+) 1182 WP_279437279.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  NYR67_RS00340 (NYR67_00340) pilF 63754..64302 (+) 549 WP_279470945.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  NYR67_RS00345 (NYR67_00345) - 64378..66717 (+) 2340 WP_279470946.1 Tex family protein -
  NYR67_RS00350 (NYR67_00350) - 66910..68190 (-) 1281 WP_279471384.1 NAD(P)/FAD-dependent oxidoreductase -
  NYR67_RS00355 (NYR67_00355) - 68302..68820 (-) 519 WP_279470947.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 182 a.a.        Molecular weight: 20668.35 Da        Isoelectric Point: 6.9479

>NTDB_id=725327 NYR67_RS00340 WP_279470945.1 63754..64302(+) (pilF) [Actinobacillus equuli subsp. equuli strain 4130]
MNLAKFLRNLTACMLLSVLFGCSSQLPTSQTTFNRSEAVKARINLALAYLEQHDLPKAKENIDKALSHDQNDYLPHSVLA
YYYQQIGDQAHAEQAYQQALKLSDNRPDVLNNYGTFLCKQAKFSQAYQQFEQAVQSEQPYYHQADSLENIVLCAKQEPNL
AKVNEALNQLEKLDKVRAAALR

Nucleotide


Download         Length: 549 bp        

>NTDB_id=725327 NYR67_RS00340 WP_279470945.1 63754..64302(+) (pilF) [Actinobacillus equuli subsp. equuli strain 4130]
ATGAACCTTGCAAAATTTTTGCGAAATTTGACCGCTTGTATGCTGTTATCTGTGCTGTTCGGTTGTTCTTCTCAGCTTCC
TACCTCTCAAACCACTTTTAATCGCTCGGAAGCGGTTAAAGCCCGTATCAATTTAGCCTTAGCGTATCTCGAGCAACATG
ATTTACCCAAAGCGAAAGAGAATATTGATAAAGCGCTAAGCCACGATCAAAACGATTACTTACCTCATTCTGTTTTAGCT
TATTACTATCAGCAAATCGGTGATCAAGCTCACGCCGAGCAAGCGTATCAGCAAGCATTGAAATTAAGTGATAATCGTCC
GGATGTCTTAAATAATTACGGCACGTTTTTGTGTAAACAAGCTAAGTTCAGTCAGGCATACCAACAATTTGAACAAGCAG
TGCAAAGCGAACAACCTTATTATCATCAAGCCGATAGCCTTGAAAATATTGTGTTATGTGCCAAACAAGAGCCGAATTTA
GCTAAAGTAAACGAAGCATTAAACCAATTAGAAAAGTTAGATAAAGTAAGAGCGGCTGCACTGCGCTAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(79-104)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

65.957

100

0.681
  pilF/pilF2 Haemophilus influenzae Rd KW20

46.784

93.956

0.44