Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   NYR65_RS00335 Genome accession   NZ_CP103811
Coordinates   63761..64309 (+) Length   182 a.a.
NCBI ID   WP_039195970.1    Uniprot ID   -
Organism   Actinobacillus equuli subsp. equuli strain CCUG2041     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 58761..69309
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR65_RS00290 (NYR65_00290) lpxK 58762..59742 (+) 981 WP_039195962.1 tetraacyldisaccharide 4'-kinase -
  NYR65_RS00295 (NYR65_00295) - 59744..59911 (+) 168 WP_039195964.1 Trm112 family protein -
  NYR65_RS00305 (NYR65_00305) gltX 60174..61613 (-) 1440 WP_039195966.1 glutamate--tRNA ligase -
  NYR65_RS00330 (NYR65_00330) - 62525..63706 (+) 1182 WP_039195968.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  NYR65_RS00335 (NYR65_00335) pilF 63761..64309 (+) 549 WP_039195970.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  NYR65_RS00340 (NYR65_00340) - 64385..66724 (+) 2340 WP_039195972.1 Tex family protein -
  NYR65_RS00345 (NYR65_00345) - 66915..68195 (-) 1281 WP_039198926.1 NAD(P)/FAD-dependent oxidoreductase -
  NYR65_RS00350 (NYR65_00350) - 68307..68825 (-) 519 WP_179130574.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 182 a.a.        Molecular weight: 20686.32 Da        Isoelectric Point: 6.9482

>NTDB_id=725273 NYR65_RS00335 WP_039195970.1 63761..64309(+) (pilF) [Actinobacillus equuli subsp. equuli strain CCUG2041]
MNLAKFLRNLTACMLLSVLFGCSSQIPTSQTTFNRSEAVKARINLALAYLEQHDLPKAKENIDKALSHDQNDYLPHSVLA
YYYQQIGDQAHAERAYQQALKLSDNRPDVLNNYGTFLCKQAKFDQAYQQFEQAVQSEQSYYHQADSLENIVLCAKQEPNL
AKVNEALNQLEKLDKARAAALR

Nucleotide


Download         Length: 549 bp        

>NTDB_id=725273 NYR65_RS00335 WP_039195970.1 63761..64309(+) (pilF) [Actinobacillus equuli subsp. equuli strain CCUG2041]
ATGAACCTTGCAAAATTTTTGCGAAATTTGACCGCTTGTATGCTGTTATCTGTGCTGTTCGGTTGTTCTTCTCAGATTCC
TACCTCTCAAACCACTTTTAATCGCTCGGAAGCGGTTAAAGCCCGTATCAATTTAGCCTTAGCGTATCTCGAGCAACATG
ATTTACCCAAAGCGAAAGAGAATATTGATAAAGCGCTAAGCCACGATCAAAACGATTACTTACCTCATTCTGTTTTAGCT
TATTACTATCAGCAAATCGGTGATCAAGCTCACGCCGAGCGAGCTTATCAGCAAGCATTGAAATTAAGTGATAATCGTCC
GGATGTATTAAATAATTACGGTACTTTTCTGTGTAAACAAGCCAAGTTTGATCAGGCATATCAACAGTTTGAGCAAGCGG
TGCAAAGCGAGCAGTCTTACTATCATCAAGCCGATAGCCTCGAAAATATTGTGTTATGTGCCAAACAAGAGCCGAATTTA
GCTAAAGTAAACGAAGCGTTAAACCAATTAGAAAAGCTGGATAAAGCAAGAGCGGCGGCACTGCGTTAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(79-104)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

66.489

100

0.687
  pilF/pilF2 Haemophilus influenzae Rd KW20

47.368

93.956

0.445