Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   NYR62_RS06420 Genome accession   NZ_CP103809
Coordinates   1439144..1439692 (-) Length   182 a.a.
NCBI ID   WP_279474965.1    Uniprot ID   -
Organism   Actinobacillus genomosp. 1 strain CCUG22229     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1434144..1444692
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR62_RS06405 (NYR62_06405) - 1434496..1435017 (+) 522 WP_279474962.1 SprT family zinc-dependent metalloprotease -
  NYR62_RS06410 (NYR62_06410) - 1435128..1436408 (+) 1281 WP_279476694.1 NAD(P)/FAD-dependent oxidoreductase -
  NYR62_RS06415 (NYR62_06415) - 1436729..1439068 (-) 2340 WP_279474963.1 Tex family protein -
  NYR62_RS06420 (NYR62_06420) pilF 1439144..1439692 (-) 549 WP_279474965.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  NYR62_RS06425 (NYR62_06425) - 1439746..1440927 (-) 1182 WP_279474968.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  NYR62_RS06450 (NYR62_06450) gltX 1441834..1443273 (+) 1440 WP_279456842.1 glutamate--tRNA ligase -
  NYR62_RS06460 (NYR62_06460) yefM 1443774..1444025 (+) 252 WP_279456843.1 YoeB-YefM toxin-antitoxin system antitoxin YefM -
  NYR62_RS06465 (NYR62_06465) - 1444022..1444282 (+) 261 WP_279474969.1 Txe/YoeB family addiction module toxin -
  NYR62_RS06470 (NYR62_06470) - 1444439..1444606 (-) 168 WP_279468242.1 Trm112 family protein -

Sequence

Protein


Download         Length: 182 a.a.        Molecular weight: 20741.44 Da        Isoelectric Point: 6.6309

>NTDB_id=725229 NYR62_RS06420 WP_279474965.1 1439144..1439692(-) (pilF) [Actinobacillus genomosp. 1 strain CCUG22229]
MNLAKFLRNLTACMLLSVLFGCSSQIPTSQTTFNRSEAVKARINLALEYLDQHDLPKAKENIDKALSHDQNDYLPHSVLA
YYYQQIGDQAHAEQAYQQALKLSDNRPDVLNNYGTFLCKQAKFDQAYQQFEQAVKSEQPYYHQADSLENIVLCAKQEPNL
AKVNEALSQLEKLDKVRAAVLR

Nucleotide


Download         Length: 549 bp        

>NTDB_id=725229 NYR62_RS06420 WP_279474965.1 1439144..1439692(-) (pilF) [Actinobacillus genomosp. 1 strain CCUG22229]
ATGAACCTTGCAAAATTTTTGCGAAATTTGACCGCTTGTATGCTGTTATCTGTGCTGTTCGGTTGTTCTTCTCAGATTCC
TACCTCTCAAACCACTTTTAATCGCTCGGAAGCGGTTAAAGCCCGTATCAATTTAGCCTTAGAGTATCTCGATCAACATG
ATTTACCCAAAGCGAAAGAGAATATTGATAAAGCACTAAGCCATGATCAAAACGATTACTTACCTCATTCTGTTTTAGCT
TATTACTATCAGCAAATCGGTGATCAAGCTCACGCCGAGCAAGCTTATCAGCAAGCATTGAAATTAAGTGATAATCGTCC
GGACGTATTAAATAATTACGGTACTTTTCTGTGTAAACAAGCCAAGTTTGATCAGGCATATCAGCAGTTTGAGCAAGCGG
TGAAAAGCGAGCAGCCTTACTATCATCAAGCCGATAGCCTCGAAAATATTGTGTTATGTGCCAAACAAGAGCCGAATTTA
GCTAAAGTAAACGAAGCATTAAGCCAATTAGAAAAGCTGGATAAAGTAAGAGCGGCGGTACTGCGCTAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(79-104)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

65.957

100

0.681
  pilF/pilF2 Haemophilus influenzae Rd KW20

46.784

93.956

0.44