Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   NYR60_RS00340 Genome accession   NZ_CP103807
Coordinates   66040..66588 (+) Length   182 a.a.
NCBI ID   WP_279467048.1    Uniprot ID   -
Organism   Actinobacillus genomosp. 2 strain CCUG15571     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 61040..71588
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR60_RS00295 (NYR60_00295) lpxK 61056..62036 (+) 981 WP_279467029.1 tetraacyldisaccharide 4'-kinase -
  NYR60_RS00300 (NYR60_00300) - 62038..62205 (+) 168 WP_005605066.1 Trm112 family protein -
  NYR60_RS00310 (NYR60_00310) gltX 62466..63905 (-) 1440 WP_279467044.1 glutamate--tRNA ligase -
  NYR60_RS00335 (NYR60_00335) - 64805..65986 (+) 1182 WP_279467046.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  NYR60_RS00340 (NYR60_00340) pilF 66040..66588 (+) 549 WP_279467048.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  NYR60_RS00345 (NYR60_00345) - 66664..69003 (+) 2340 WP_279467050.1 Tex family protein -
  NYR60_RS00350 (NYR60_00350) - 69273..69560 (+) 288 WP_279467051.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
  NYR60_RS00355 (NYR60_00355) - 69560..69940 (+) 381 WP_279467052.1 type II toxin-antitoxin system VapC family toxin -
  NYR60_RS00360 (NYR60_00360) - 70049..71329 (-) 1281 WP_279467986.1 NAD(P)/FAD-dependent oxidoreductase -

Sequence

Protein


Download         Length: 182 a.a.        Molecular weight: 20843.49 Da        Isoelectric Point: 6.6299

>NTDB_id=725169 NYR60_RS00340 WP_279467048.1 66040..66588(+) (pilF) [Actinobacillus genomosp. 2 strain CCUG15571]
MNFAKFLRNLTACMLLFVLFGCSSQLPTYQTTFNRSEAVKARINLALAYLDQHDLSKAKENIDKALSHDQNDYLPHSVLA
YYYQQIGDQVHAEQAYQQALRLSDNRPDVLNNYGTFLCKQAKFDQAYQQFEQAVQSEQPYYHQADSLENIVLCAKQEPNL
AKVNEALSQLEKLDKARAAALR

Nucleotide


Download         Length: 549 bp        

>NTDB_id=725169 NYR60_RS00340 WP_279467048.1 66040..66588(+) (pilF) [Actinobacillus genomosp. 2 strain CCUG15571]
ATGAACTTTGCAAAATTTTTGCGAAATTTGACCGCTTGTATGCTGTTATTTGTGCTGTTCGGTTGTTCTTCTCAGCTTCC
TACCTACCAAACTACCTTTAATCGTTCGGAAGCGGTTAAAGCCCGTATCAATTTAGCCTTAGCGTATCTCGATCAACATG
ATTTATCCAAAGCGAAAGAGAATATTGATAAAGCATTAAGCCACGATCAAAACGATTACTTACCTCATTCTGTTTTAGCT
TATTACTATCAGCAAATCGGTGATCAAGTTCATGCGGAACAGGCTTATCAGCAAGCATTGAGATTAAGTGATAATCGCCC
GGACGTATTAAATAATTACGGTACTTTTCTGTGTAAACAAGCTAAGTTTGATCAGGCATATCAGCAGTTTGAGCAAGCGG
TGCAAAGCGAGCAGCCTTACTATCATCAAGCCGATAGTCTCGAAAATATCGTGTTGTGTGCCAAACAAGAGCCGAATCTA
GCTAAAGTAAACGAAGCGTTAAGCCAATTAGAAAAGCTGGATAAAGCAAGAGCTGCAGCACTGCGTTAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(79-104)

Tetratricopeptide repeat (TPR_8)

(40-70)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

65.957

100

0.681
  pilF/pilF2 Haemophilus influenzae Rd KW20

46.784

93.956

0.44