Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   M52SOB_RS13700 Genome accession   NZ_AP019373
Coordinates   2710090..2711535 (+) Length   481 a.a.
NCBI ID   WP_131112331.1    Uniprot ID   -
Organism   Sulfuricystis thermophila strain M52     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2705090..2716535
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M52SOB_RS13665 nusB 2705482..2705931 (+) 450 WP_172601855.1 transcription antitermination factor NusB -
  M52SOB_RS13670 thiL 2705944..2706879 (+) 936 WP_131112325.1 thiamine-phosphate kinase -
  M52SOB_RS13675 - 2706886..2707389 (+) 504 WP_131112326.1 phosphatidylglycerophosphatase A -
  M52SOB_RS13680 - 2707391..2707885 (+) 495 WP_131112327.1 nicotinamide-nucleotide amidohydrolase family protein -
  M52SOB_RS13685 recA 2707913..2708932 (+) 1020 WP_131112328.1 recombinase RecA Machinery gene
  M52SOB_RS13690 recX 2708925..2709353 (+) 429 WP_131112329.1 recombination regulator RecX -
  M52SOB_RS13695 lptB 2709350..2710084 (+) 735 WP_131112330.1 LPS export ABC transporter ATP-binding protein -
  M52SOB_RS13700 rpoN/rpoN1 2710090..2711535 (+) 1446 WP_131112331.1 RNA polymerase factor sigma-54 Machinery gene
  M52SOB_RS13705 raiA 2711549..2711860 (+) 312 WP_131112332.1 ribosome-associated translation inhibitor RaiA -
  M52SOB_RS13710 ptsN 2711990..2712451 (+) 462 WP_131112333.1 PTS IIA-like nitrogen regulatory protein PtsN -
  M52SOB_RS13715 hprK 2712439..2713377 (+) 939 WP_131112556.1 HPr(Ser) kinase/phosphatase -
  M52SOB_RS13720 - 2713449..2713871 (+) 423 WP_131112334.1 DUF971 domain-containing protein -
  M52SOB_RS13725 ubiE 2713880..2714614 (+) 735 WP_284155253.1 bifunctional demethylmenaquinone methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol methylase UbiE -
  M52SOB_RS13730 - 2714779..2715354 (+) 576 WP_131112336.1 SCP2 sterol-binding domain-containing protein -
  M52SOB_RS13735 - 2715340..2716317 (-) 978 WP_131112337.1 PAS domain S-box protein -

Sequence

Protein


Download         Length: 481 a.a.        Molecular weight: 53203.16 Da        Isoelectric Point: 4.8998

>NTDB_id=72513 M52SOB_RS13700 WP_131112331.1 2710090..2711535(+) (rpoN/rpoN1) [Sulfuricystis thermophila strain M52]
MKQTLQLKLSQHLALTPQLQQSIRLLQLSTLELNQEIEQALQDNPLLERAEPDEAQVFGGSPDALSHATEPAPSAGEERI
ETSAEWGEDGLEWPSAGAKGPRDPNDENDADAGELQAAATSLTDHLIAQLALSQLAGRDRLLVAFLIEALDEDGYLDQPL
EELAEVLPAELIGEPDEVIEDLQIALKHLQSLDPTGVGARDARECLLLQLANLPATPERTLAMTIVRDHLEQLAAHDYAR
IKKATGCDDEALRSAQELIRSLNPRPGAQFASLDTRYVVPDVIVRKLRGQWVAQLNQDVMPRLRINRQYADLLRRHRNSG
SSNLTGQLQEAKWLIKNVQQRFDTIARVAQAIVDRQRAFFDHGEVAMRPLTLREIAETVDLHESTISRVTTQKYLACPRG
IYELKYFFGSHVATDTGGAASSTAIRALIRQLVAAEDGQKPLSDARIADILGEQGFKVARRTVAKYRESLGIPPVNLRKT
L

Nucleotide


Download         Length: 1446 bp        

>NTDB_id=72513 M52SOB_RS13700 WP_131112331.1 2710090..2711535(+) (rpoN/rpoN1) [Sulfuricystis thermophila strain M52]
GTGAAGCAAACGCTCCAGCTCAAGCTGTCGCAGCATCTCGCACTGACGCCGCAGTTGCAGCAGTCGATCCGTCTGTTGCA
ATTGTCGACGCTGGAGCTCAACCAGGAAATCGAGCAGGCGCTGCAGGACAATCCATTGCTCGAACGGGCGGAGCCGGATG
AGGCTCAAGTCTTCGGCGGCTCGCCGGATGCCTTGAGCCATGCCACTGAGCCAGCGCCGTCCGCTGGCGAGGAGCGCATC
GAGACCAGCGCCGAATGGGGCGAGGATGGCTTGGAATGGCCCAGCGCTGGCGCCAAAGGACCGCGCGATCCGAACGATGA
GAACGATGCCGATGCCGGCGAACTGCAGGCCGCCGCGACGAGTCTCACCGATCACCTGATCGCCCAGTTGGCCCTCTCGC
AGCTTGCCGGGCGCGACCGGCTGCTGGTGGCGTTCCTGATCGAAGCGCTCGACGAGGACGGCTATCTCGACCAGCCGCTC
GAGGAGCTCGCCGAGGTGCTGCCCGCCGAGCTGATCGGCGAGCCCGACGAAGTCATCGAGGACTTGCAGATCGCGCTCAA
ACATTTACAAAGTCTCGACCCAACGGGCGTCGGCGCACGCGATGCGCGCGAATGCCTGCTGCTCCAGCTCGCCAACTTGC
CGGCCACCCCGGAACGCACGCTGGCGATGACGATCGTGCGCGACCACCTCGAACAGCTGGCGGCGCACGATTACGCGCGC
ATCAAAAAGGCGACGGGCTGTGACGACGAGGCATTGCGCAGCGCCCAGGAATTGATCCGTTCGCTCAACCCGCGGCCGGG
CGCGCAATTCGCGTCGCTCGACACGCGCTATGTCGTGCCCGACGTGATCGTCCGGAAATTGCGCGGCCAGTGGGTTGCCC
AGTTGAATCAGGATGTCATGCCGCGCCTGCGCATCAACCGTCAATACGCCGATCTGCTGCGCCGGCATCGCAATAGCGGC
AGCAGCAATCTCACCGGCCAGCTGCAGGAAGCCAAGTGGCTGATCAAGAACGTGCAGCAGCGTTTCGATACCATCGCACG
TGTCGCGCAAGCCATCGTCGATCGCCAGCGCGCCTTCTTCGATCACGGCGAGGTCGCGATGCGCCCGCTGACACTGCGCG
AGATCGCCGAAACGGTCGATCTGCACGAATCGACCATCTCGCGCGTGACGACGCAGAAATACCTCGCCTGTCCACGCGGC
ATCTATGAACTCAAATATTTTTTCGGCAGCCACGTCGCCACCGATACCGGCGGCGCGGCTTCATCCACCGCGATCCGGGC
GCTGATCAGGCAGCTCGTCGCCGCCGAAGACGGCCAAAAGCCGCTTTCGGACGCGCGCATCGCGGATATCCTCGGCGAAC
AGGGCTTCAAGGTCGCGCGGCGCACCGTCGCGAAGTATCGTGAATCCCTTGGCATCCCGCCGGTCAACCTTCGCAAAACT
CTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(324-479)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(114-309)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

58.893

100

0.62

Multiple sequence alignment