Detailed information NTG0725115

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: NYS58_RS26815
• Genome accession: NZ_CP103790
• Coordinates: 5651404..5652276 (+)
• Length: 290 a.a.
• NCBI ID: WP_003141356.1
• Uniprot ID: A0A0H2ZGZ1
• Organism: Pseudomonas aeruginosa strain 8247
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5646404..5657276

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NYS58_RS26795	-	5646947..5647645 (-)	699	WP_016254081.1	hypothetical protein	-
NYS58_RS26800	-	5647709..5648248 (-)	540	WP_003141352.1	pilin	-
NYS58_RS26805	pilB	5648477..5650180 (+)	1704	WP_003141354.1	type IV-A pilus assembly ATPase PilB	Machinery gene
NYS58_RS26810	pilC	5650184..5651401 (+)	1218	WP_016254080.1	type II secretion system F family protein	Machinery gene
NYS58_RS26815	pilD	5651404..5652276 (+)	873	WP_003141356.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
NYS58_RS26820	coaE	5652273..5652884 (+)	612	WP_003094654.1	dephospho-CoA kinase	-
NYS58_RS26825	yacG	5652881..5653081 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-
NYS58_RS26830	-	5653118..5653327 (-)	210	WP_003094660.1	hypothetical protein	-
NYS58_RS26835	-	5653433..5654122 (-)	690	WP_003103868.1	energy-coupling factor ABC transporter permease	-
NYS58_RS26840	-	5654119..5654589 (-)	471	WP_003094664.1	hypothetical protein	-
NYS58_RS26845	-	5654586..5655011 (-)	426	WP_003103865.1	GNAT family N-acetyltransferase	-
NYS58_RS26850	-	5655144..5655773 (+)	630	WP_003094668.1	DUF1780 domain-containing protein	-
NYS58_RS26855	-	5655770..5656219 (+)	450	WP_003141357.1	MOSC domain-containing protein	-
NYS58_RS26860	-	5656245..5656415 (+)	171	WP_003094672.1	DUF3094 family protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31813.85 Da
• Isoelectric Point: 7.7068

>NTDB_id=725115 NYS58_RS26815 WP_003141356.1 5651404..5652276(+) (pilD) [Pseudomonas aeruginosa strain 8247]
MELLDYLASHPLAFVLCAILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQATYNLVLPNSACPRCGHEIR
PWENIPLVSYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLGDALFGAVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide

• Length: 873 bp

>NTDB_id=725115 NYS58_RS26815 WP_003141356.1 5651404..5652276(+) (pilD) [Pseudomonas aeruginosa strain 8247]
ATGGAACTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCGCCATCCTCCTTGGCCTGCTGGTCGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAGGCCCGCGAAGCGCTCGGGC
TGGAGCCGGAACCCAAGCAGGCGACCTACAACCTGGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCCACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGTCAGCTACCTGGCGCTGGGCGGCAAATGCTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCTGGCTACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGTCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTGCCGCTGCTCTGGCTGGGCCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGGCGATGCGCTGTTCGGCGCGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGCGGCTGGCAGATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCCTTCGGTCCCTATCTGGCGAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

3D structure

• AlphaFold DB: A0A0H2ZGZ1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	55.017	99.655	0.548
pilD	Vibrio campbellii strain DS40M4	53.497	98.621	0.528
pilD	Acinetobacter nosocomialis M2	50.352	97.931	0.493
pilD	Acinetobacter baumannii D1279779	50.352	97.931	0.493
pilD	Neisseria gonorrhoeae MS11	49.281	95.862	0.472