Detailed information NTG0724387

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: M5R33_RS21500
• Genome accession: NZ_CP103729
• Coordinates: 4319369..4320169 (+)
• Length: 266 a.a.
• NCBI ID: WP_004145898.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain 197
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4314369..4325169

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M5R33_RS21465 (M5R33_21465)	gspH	4314525..4315040 (+)	516	WP_004191951.1	type II secretion system minor pseudopilin GspH	-
M5R33_RS21470 (M5R33_21470)	gspI	4315037..4315402 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
M5R33_RS21475 (M5R33_21475)	gspJ	4315399..4315995 (+)	597	WP_038807639.1	type II secretion system minor pseudopilin GspJ	-
M5R33_RS21480 (M5R33_21480)	gspK	4315992..4316978 (+)	987	WP_077258272.1	type II secretion system minor pseudopilin GspK	-
M5R33_RS21485 (M5R33_21485)	gspL	4316965..4318158 (+)	1194	WP_048255484.1	type II secretion system protein GspL	-
M5R33_RS21490 (M5R33_21490)	-	4318151..4318636 (+)	486	WP_004151943.1	type II secretion system protein M	-
M5R33_RS21495 (M5R33_21495)	-	4318626..4319372 (+)	747	WP_009309263.1	type II secretion system protein N	-
M5R33_RS21500 (M5R33_21500)	pilD	4319369..4320169 (+)	801	WP_004145898.1	A24 family peptidase	Machinery gene
M5R33_RS21505 (M5R33_21505)	hrpB	4320217..4322646 (-)	2430	WP_048255483.1	ATP-dependent helicase HrpB	-
M5R33_RS21510 (M5R33_21510)	thpR	4322719..4323255 (+)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
M5R33_RS21515 (M5R33_21515)	sfsA	4323255..4323971 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
M5R33_RS21520 (M5R33_21520)	dksA	4324134..4324589 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29389.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=724387 M5R33_RS21500 WP_004145898.1 4319369..4320169(+) (pilD) [Klebsiella pneumoniae strain 197]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=724387 M5R33_RS21500 WP_004145898.1 4319369..4320169(+) (pilD) [Klebsiella pneumoniae strain 197]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGATCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425