Detailed information NTG0072422

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: KU43P_RS03410
• Genome accession: NZ_AP019365
• Coordinates: 756007..756873 (-)
• Length: 288 a.a.
• NCBI ID: WP_317661080.1
• Uniprot ID: -
• Organism: Pseudomonas sp. KU43P
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 751007..761873

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KU43P_RS03380 (KU43P_06690)	-	752046..753341 (-)	1296	WP_317661077.1	NAD(P)/FAD-dependent oxidoreductase	-
KU43P_RS03385	-	753426..753599 (-)	174	WP_008091951.1	DUF3094 domain-containing protein	-
KU43P_RS03390 (KU43P_06710)	-	753732..754361 (-)	630	WP_008091953.1	DUF1780 domain-containing protein	-
KU43P_RS03395 (KU43P_06720)	-	754449..755141 (+)	693	WP_317661078.1	energy-coupling factor ABC transporter permease	-
KU43P_RS03400 (KU43P_06730)	yacG	755187..755387 (-)	201	WP_176513869.1	DNA gyrase inhibitor YacG	-
KU43P_RS03405 (KU43P_06740)	coaE	755384..756007 (-)	624	WP_317661079.1	dephospho-CoA kinase	-
KU43P_RS03410 (KU43P_06750)	pilD	756007..756873 (-)	867	WP_317661080.1	prepilin peptidase	Machinery gene
KU43P_RS03415 (KU43P_06760)	-	756876..758084 (-)	1209	WP_317661081.1	type II secretion system F family protein	-
KU43P_RS03420 (KU43P_06770)	-	758303..758713 (+)	411	WP_317661082.1	pilin	-
KU43P_RS03430 (KU43P_06780)	-	759021..760310 (+)	1290	WP_083567633.1	tyrosine-type recombinase/integrase	-
KU43P_RS03435 (KU43P_06800)	-	760581..761330 (+)	750	WP_317661083.1	hypothetical protein	-
KU43P_RS03440 (KU43P_06810)	-	761429..761689 (+)	261	WP_154211779.1	helix-turn-helix transcriptional regulator	-

Sequence

Protein

• Length: 288 a.a.
• Molecular weight: 31866.92 Da
• Isoelectric Point: 6.9096

>NTDB_id=72422 KU43P_RS03410 WP_317661080.1 756007..756873(-) (pilD) [Pseudomonas sp. KU43P]
MTLWTFLAEQPAYFITLATLLGLLVGSFVNVVVYRLPIMLERQWQHEAQEVLGLEHHAHERFDLCLPASHCPHCGHRIRA
WENIPLLSYLLLRGRCSACKSAISPRYPLVEAACAVLSLVVAWHFGPTLEALLALPLTWCLLALSLIDADHSLLPDVLVL
PTLWLGLIVNAFAVFVPLTNALWGAVAGYLSLWTVYWLFKLVTGKEGMGYGDFKLLALIGAWAGWHVLPLTLLLSSLLGA
VIGLSLLRLRHHPVNVAIPFGPYLAIAGWIAVLWGDEIYASYLQLLGL

Nucleotide

• Length: 867 bp

>NTDB_id=72422 KU43P_RS03410 WP_317661080.1 756007..756873(-) (pilD) [Pseudomonas sp. KU43P]
ATGACTTTATGGACTTTCCTGGCTGAGCAGCCGGCCTACTTCATCACCCTGGCGACCTTGCTCGGCCTGTTGGTGGGCAG
CTTCGTCAATGTGGTGGTGTATCGCCTGCCGATCATGCTGGAGCGCCAATGGCAACACGAGGCCCAGGAGGTGCTGGGGC
TCGAGCACCATGCGCACGAGCGCTTCGACCTGTGCCTGCCGGCATCCCACTGCCCCCATTGTGGGCACCGTATCCGTGCG
TGGGAGAACATTCCCCTGTTGAGCTATCTGCTGCTGCGGGGGCGCTGCTCGGCCTGTAAAAGCGCCATCAGCCCACGCTA
TCCGCTGGTGGAGGCTGCCTGCGCGGTGCTGTCACTGGTGGTGGCCTGGCATTTCGGCCCCACGCTCGAAGCCCTGCTGG
CATTGCCTTTGACCTGGTGCTTGCTGGCCCTGAGCCTGATCGATGCCGACCACAGCCTGTTGCCGGACGTGCTGGTGCTG
CCGACGCTGTGGCTGGGCCTGATCGTGAATGCCTTTGCCGTCTTCGTGCCGCTGACCAATGCCTTGTGGGGCGCGGTGGC
CGGGTACCTCAGCCTGTGGACGGTGTACTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGTTATGGCGACTTCA
AGCTGCTGGCGTTGATCGGAGCCTGGGCTGGGTGGCATGTATTGCCGCTGACCTTGCTGTTATCGTCGCTGCTGGGTGCG
GTAATCGGCCTAAGCCTGCTGCGTCTGCGCCACCACCCGGTGAACGTGGCGATACCCTTTGGGCCTTATCTGGCCATTGC
GGGGTGGATTGCCGTGCTCTGGGGTGATGAAATATACGCCTCCTACCTGCAACTGCTTGGACTCTGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-244)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-125)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	53.597	96.528	0.517
pilD	Acinetobacter baumannii D1279779	52.5	97.222	0.51
pilD	Acinetobacter nosocomialis M2	52.5	97.222	0.51
pilD	Vibrio campbellii strain DS40M4	48.375	96.181	0.465
pilD	Neisseria gonorrhoeae MS11	48.708	94.097	0.458