Detailed information NTG0724194

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: M5R27_RS15300
• Genome accession: NZ_CP103697
• Coordinates: 3146464..3147264 (+)
• Length: 266 a.a.
• NCBI ID: WP_032414527.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain ST3576
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3141464..3152264

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M5R27_RS15265 (M5R27_15265)	gspH	3141620..3142135 (+)	516	WP_032414529.1	type II secretion system minor pseudopilin GspH	-
M5R27_RS15270 (M5R27_15270)	gspI	3142132..3142497 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
M5R27_RS15275 (M5R27_15275)	gspJ	3142494..3143090 (+)	597	WP_004145893.1	type II secretion system minor pseudopilin GspJ	-
M5R27_RS15280 (M5R27_15280)	gspK	3143087..3144073 (+)	987	WP_077254427.1	type II secretion system minor pseudopilin GspK	-
M5R27_RS15285 (M5R27_15285)	gspL	3144060..3145253 (+)	1194	WP_121915433.1	type II secretion system protein GspL	-
M5R27_RS15290 (M5R27_15290)	-	3145246..3145731 (+)	486	WP_004145896.1	type II secretion system protein M	-
M5R27_RS15295 (M5R27_15295)	-	3145721..3146467 (+)	747	WP_009309263.1	type II secretion system protein N	-
M5R27_RS15300 (M5R27_15300)	pilD	3146464..3147264 (+)	801	WP_032414527.1	A24 family peptidase	Machinery gene
M5R27_RS15305 (M5R27_15305)	hrpB	3147312..3149741 (-)	2430	WP_032414526.1	ATP-dependent helicase HrpB	-
M5R27_RS15310 (M5R27_15310)	thpR	3149814..3150350 (+)	537	WP_032414524.1	RNA 2',3'-cyclic phosphodiesterase	-
M5R27_RS15315 (M5R27_15315)	sfsA	3150350..3151066 (+)	717	WP_004177390.1	DNA/RNA nuclease SfsA	-
M5R27_RS15320 (M5R27_15320)	dksA	3151229..3151684 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29419.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=724194 M5R27_RS15300 WP_032414527.1 3146464..3147264(+) (pilD) [Klebsiella pneumoniae strain ST3576]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPISWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMI

Nucleotide

• Length: 801 bp

>NTDB_id=724194 M5R27_RS15300 WP_032414527.1 3146464..3147264(+) (pilD) [Klebsiella pneumoniae strain ST3576]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCTCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAACTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGATCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.057	99.624	0.489
pilD	Vibrio campbellii strain DS40M4	46.545	100	0.481
pilD	Acinetobacter nosocomialis M2	46.388	98.872	0.459
pilD	Acinetobacter baumannii D1279779	45.627	98.872	0.451
pilD	Neisseria gonorrhoeae MS11	43.922	95.865	0.421