Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   KU43P_RS01500 Genome accession   NZ_AP019365
Coordinates   313839..316058 (-) Length   739 a.a.
NCBI ID   WP_317660737.1    Uniprot ID   A0A5K7TQ14
Organism   Pseudomonas sp. KU43P     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 308839..321058
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KU43P_RS01475 (KU43P_02920) pilT 308843..309841 (+) 999 WP_317660732.1 type IV pilus twitching motility protein PilT Machinery gene
  KU43P_RS01480 (KU43P_02930) - 309951..310580 (-) 630 WP_317660733.1 C40 family peptidase -
  KU43P_RS01485 (KU43P_02940) - 310750..311169 (+) 420 WP_317660734.1 NINE protein -
  KU43P_RS01490 (KU43P_02950) - 311219..311917 (-) 699 WP_317660735.1 SPOR domain-containing protein -
  KU43P_RS01495 (KU43P_02960) argS 311919..313655 (-) 1737 WP_317660736.1 arginine--tRNA ligase -
  KU43P_RS01500 (KU43P_02970) priA 313839..316058 (-) 2220 WP_317660737.1 primosomal protein N' Machinery gene
  KU43P_RS01505 (KU43P_02980) rpmE 316226..316441 (+) 216 WP_008092997.1 50S ribosomal protein L31 -
  KU43P_RS01510 (KU43P_02990) - 316444..317265 (+) 822 WP_317660738.1 thermonuclease family protein -
  KU43P_RS01515 (KU43P_03000) - 317446..318714 (+) 1269 WP_317660739.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 739 a.a.        Molecular weight: 82494.15 Da        Isoelectric Point: 8.3031

>NTDB_id=72419 KU43P_RS01500 WP_317660737.1 313839..316058(-) (priA) [Pseudomonas sp. KU43P]
MSDVILRLALPSPLRRLFDYRAPASMARQALVPGMRIRVPFGRREMIGVLVEVCEQSEVPADKLKPAIALLDPVTPVPAA
LFKLCLWTAQYYQHSLGDTLSWALPTLLRQGEPAEMRQERFWHVAPGARLEDPRIARAPRQRDALKTLAQHPHGVAHSLL
AKLNLNKDSLDLLLAKELVQVEVRRQRPAERHEHWLAQPELPLNEEQRDAFDAVREGFGEFGAFLLAGVTGSGKTEVYLQ
LIREALEAGKQALVLIPEINLGPQTLARFEQRFNARIALLHSAVNDRERLDAWLAARDGEADIIIGTRSALFTPMKNPGL
IIIDEEHDGSYKQQEGLRYHARDLALVRAHQENIPILLGSATPSLETLHNALTGRYRLLRMNQRAGGARPPRMLRLDVKS
LPLDSGISGPLQQAIRLTLEAGQQVLVFLNRRGFAPTLLCHDCGWLSECPRCDARMTVHQRSGVLRCHHCGYDERLPHQC
PKCNHVDLRPVGAGTERAEERLKVLFPDYPILRIDRDSTARKDAMQNLFSTIQRGQPSILVGTQMLAKGHHFPRVTLVAI
LDADGGLFSGDFRASERMAQLIVQVAGRAGRAEEPGKVIIQTHLADHPLLVQLTEQGYFAFAEQALDERRAAGLPPYSHL
ALLRAEAHKPGQAEGFLDEACSTAERLVAEQQLAGIELLGPVPAPMERKAGRFRAQLLIQANTRAPLHRLISAWLLVLEQ
MPSGRQVRWSLDVDPVDLY

Nucleotide


Download         Length: 2220 bp        

>NTDB_id=72419 KU43P_RS01500 WP_317660737.1 313839..316058(-) (priA) [Pseudomonas sp. KU43P]
GTGTCCGACGTCATCCTGCGCCTTGCCCTGCCCTCCCCTCTGCGTCGCCTGTTCGACTACCGAGCGCCGGCGAGCATGGC
GCGCCAGGCCCTGGTACCGGGCATGCGGATCCGCGTGCCGTTCGGCCGCCGCGAGATGATCGGCGTGCTGGTGGAAGTGT
GCGAGCAGAGTGAAGTGCCGGCCGACAAGCTCAAGCCCGCCATTGCCCTGCTCGACCCGGTAACGCCGGTGCCTGCGGCC
CTTTTCAAGCTGTGCCTGTGGACAGCCCAGTATTATCAGCACAGCCTGGGCGACACCCTGAGCTGGGCCTTGCCCACGCT
GCTGCGCCAGGGTGAGCCCGCCGAGATGCGCCAGGAGCGCTTCTGGCACGTTGCCCCCGGTGCCCGCCTGGAAGACCCGC
GCATCGCCCGCGCCCCACGTCAACGCGACGCCCTGAAAACCCTGGCCCAGCACCCCCATGGTGTGGCCCATAGCCTGCTG
GCCAAGCTCAACCTGAACAAGGACAGCCTCGACCTGCTGCTGGCCAAGGAGCTGGTGCAGGTGGAAGTGCGCCGCCAGCG
CCCGGCAGAGCGCCATGAACACTGGCTGGCCCAGCCGGAGCTGCCGCTCAACGAAGAACAGCGCGATGCCTTCGACGCCG
TGCGCGAGGGCTTTGGCGAGTTCGGCGCGTTTCTCCTGGCAGGCGTCACCGGCAGCGGCAAGACCGAGGTCTACCTGCAA
CTCATCCGCGAGGCCCTCGAAGCCGGCAAGCAGGCACTGGTACTGATTCCCGAGATCAACCTCGGCCCGCAGACCCTGGC
GCGCTTCGAGCAGCGCTTCAATGCCCGCATCGCCCTGCTGCACTCGGCGGTGAACGATCGCGAGCGTCTCGATGCCTGGC
TGGCAGCGCGCGACGGCGAGGCCGACATCATCATCGGCACACGCTCGGCGCTGTTCACGCCGATGAAGAACCCAGGTCTG
ATCATCATCGACGAGGAACACGACGGCTCCTATAAACAGCAGGAGGGCCTGCGTTACCACGCCCGCGACTTGGCGCTGGT
GCGCGCACATCAGGAAAACATCCCGATCCTGCTTGGCTCCGCCACTCCCTCACTGGAAACGCTGCACAACGCCCTGACCG
GCCGTTATCGCCTGCTGCGCATGAACCAGCGCGCCGGTGGCGCACGCCCACCACGCATGCTACGCCTGGACGTGAAGAGC
CTGCCGCTGGACAGCGGCATCAGTGGCCCGCTGCAGCAGGCCATCCGCCTTACCCTGGAAGCCGGCCAGCAAGTGCTCGT
GTTCCTCAACCGCCGCGGCTTCGCTCCCACCTTGCTGTGCCATGACTGCGGCTGGCTGTCCGAGTGCCCGCGTTGCGATG
CGCGCATGACGGTGCACCAACGCTCCGGTGTGCTGCGCTGCCACCACTGCGGCTATGACGAACGCCTGCCGCACCAGTGC
CCCAAGTGCAACCACGTGGACTTGCGACCGGTCGGCGCCGGCACCGAACGTGCCGAGGAACGCTTGAAAGTGCTGTTCCC
CGATTACCCGATCCTGCGCATTGACCGCGACAGCACGGCGCGCAAGGACGCCATGCAGAACTTGTTCAGCACCATCCAGC
GCGGCCAGCCGAGCATCCTGGTCGGCACCCAGATGCTGGCCAAGGGGCACCATTTCCCGCGGGTAACGCTGGTGGCAATC
CTTGATGCCGATGGAGGGCTGTTCTCAGGTGATTTCCGCGCCAGCGAGCGCATGGCCCAGCTCATCGTCCAGGTCGCCGG
CCGCGCCGGCCGTGCCGAGGAGCCCGGCAAGGTGATCATCCAGACCCACCTGGCCGACCACCCGTTGCTGGTGCAGTTGA
CCGAGCAAGGCTACTTCGCCTTCGCCGAACAGGCCCTGGACGAACGCCGCGCAGCCGGCCTGCCGCCTTACTCGCACCTG
GCCCTGCTGCGCGCCGAAGCGCACAAGCCAGGCCAGGCAGAGGGTTTTCTCGACGAGGCCTGCAGCACAGCGGAGCGCCT
GGTGGCCGAGCAACAACTGGCGGGCATTGAACTGTTGGGCCCGGTACCGGCTCCGATGGAGCGCAAGGCGGGACGCTTCC
GCGCGCAACTGTTGATCCAGGCCAATACACGGGCACCCTTGCATCGACTGATCAGTGCCTGGTTGCTAGTGTTGGAGCAG
ATGCCGAGCGGGCGCCAGGTACGCTGGTCGCTGGATGTGGATCCGGTAGACCTGTACTGA

Domains

Predicted by InterproScan.

3DNA-binding domain (3BD) (PriA_3primeBD)

(9-105)

Primosomal protein N C-terminal domain (PriA_C)

(636-735)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(449-475)

DEAD/DEAH box helicase (DEAD)

(207-368)

Helicase conserved C-terminal domain (Helicase_C)

(520-592)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5K7TQ14

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

48.243

100

0.483

Multiple sequence alignment