Detailed information NTG0724156

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: M5T43_RS22810
• Genome accession: NZ_CP103690
• Coordinates: 4830175..4830972 (+)
• Length: 265 a.a.
• NCBI ID: WP_023320340.1
• Uniprot ID: -
• Organism: Klebsiella oxytoca strain ST34
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4825175..4835972

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M5T43_RS22775 (M5T43_22775)	gspH	4825319..4825831 (+)	513	WP_023320342.1	type II secretion system minor pseudopilin GspH	-
M5T43_RS22780 (M5T43_22780)	gspI	4825828..4826193 (+)	366	WP_004110668.1	type II secretion system minor pseudopilin GspI	-
M5T43_RS22785 (M5T43_22785)	gspJ	4826190..4826786 (+)	597	WP_004107987.1	type II secretion system minor pseudopilin GspJ	-
M5T43_RS22790 (M5T43_22790)	gspK	4826783..4827763 (+)	981	WP_023320341.1	type II secretion system minor pseudopilin GspK	-
M5T43_RS22795 (M5T43_22795)	gspL	4827756..4828946 (+)	1191	WP_004107991.1	type II secretion system protein GspL	-
M5T43_RS22800 (M5T43_22800)	gspM	4828939..4829424 (+)	486	WP_004107993.1	type II secretion system protein GspM	-
M5T43_RS22805 (M5T43_22805)	-	4829414..4830172 (+)	759	WP_004107995.1	type II secretion system protein N	-
M5T43_RS22810 (M5T43_22810)	pilD	4830175..4830972 (+)	798	WP_023320340.1	prepilin peptidase	Machinery gene
M5T43_RS22815 (M5T43_22815)	hrpB	4831070..4833499 (-)	2430	WP_023320339.1	ATP-dependent helicase HrpB	-
M5T43_RS22820 (M5T43_22820)	thpR	4833572..4834108 (+)	537	WP_023320338.1	RNA 2',3'-cyclic phosphodiesterase	-
M5T43_RS22825 (M5T43_22825)	sfsA	4834108..4834824 (+)	717	WP_004108004.1	DNA/RNA nuclease SfsA	-
M5T43_RS22830 (M5T43_22830)	dksA	4834989..4835444 (+)	456	WP_004098689.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 265 a.a.
• Molecular weight: 29180.93 Da
• Isoelectric Point: 8.3391

>NTDB_id=724156 M5T43_RS22810 WP_023320340.1 4830175..4830972(+) (pilD) [Klebsiella oxytoca strain ST34]
MENIALLSEFAAQFPLIWGGFLFLSGLAFGSFFNVVIHRLPLMMEYAEGVNLCFPASFCPQCRQPIAWRDNIPLLSFLWL
KGRSRCCGRPISRRYPLMELATGALFMLVGYLIPAGMPLLGGLILLSLLLILAAIDAQTQLLPDRLTLPLLWAGLLFNLT
DLFAPLAEAVVGAMAGYLSLWSVYWVFRLLTGKEALGYGDFKLLAALGAWLGWQALPHTLLLASASGLIWTLLQRLVTRR
SLEQPLAFGPWLALAGGGIFLWGQV

Nucleotide

• Length: 798 bp

>NTDB_id=724156 M5T43_RS22810 WP_023320340.1 4830175..4830972(+) (pilD) [Klebsiella oxytoca strain ST34]
GTGGAAAACATCGCATTGTTATCCGAGTTCGCCGCACAATTCCCGTTAATCTGGGGCGGTTTTTTATTCTTGTCCGGACT
GGCGTTCGGCAGCTTTTTTAACGTCGTTATCCATCGTTTACCGCTGATGATGGAGTACGCAGAGGGAGTGAATCTCTGCT
TCCCCGCATCGTTCTGTCCGCAGTGCCGTCAGCCCATCGCCTGGCGGGACAATATCCCGCTGCTGAGCTTTTTATGGCTC
AAGGGGCGTTCGCGCTGCTGCGGGCGACCGATTTCACGTCGCTATCCGCTGATGGAGCTGGCGACCGGCGCGCTGTTTAT
GCTGGTCGGGTACCTGATCCCTGCGGGCATGCCGCTGCTGGGCGGGTTAATTTTATTGTCGCTGCTGCTGATCCTCGCCG
CCATCGACGCGCAAACGCAGCTGCTGCCTGACAGGCTGACTCTGCCGCTGCTGTGGGCCGGGCTGCTGTTCAATCTTACC
GATCTCTTCGCGCCGCTGGCGGAGGCGGTCGTTGGCGCGATGGCCGGGTATCTATCGCTGTGGTCAGTGTACTGGGTGTT
CCGTCTGCTGACCGGTAAAGAGGCGCTGGGCTACGGTGATTTCAAGCTGCTGGCGGCGCTCGGCGCGTGGCTCGGCTGGC
AGGCGCTGCCGCATACGCTGCTGCTGGCTTCCGCCAGCGGCCTGATCTGGACGCTGCTGCAGCGCCTGGTAACCCGTCGC
TCGCTCGAACAGCCTCTGGCCTTCGGCCCCTGGCTGGCGCTGGCGGGCGGCGGCATCTTTTTGTGGGGCCAGGTATAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (25-110)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.042	98.491	0.483
pilD	Neisseria gonorrhoeae MS11	45.324	100	0.475
pilD	Vibrio campbellii strain DS40M4	43.841	100	0.457
pilD	Acinetobacter nosocomialis M2	45.174	97.736	0.442
pilD	Acinetobacter baumannii D1279779	44.788	97.736	0.438