Detailed information NTG0724132

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: M5S48_RS21450
• Genome accession: NZ_CP103687
• Coordinates: 4368100..4368900 (+)
• Length: 266 a.a.
• NCBI ID: WP_004213441.1
• Uniprot ID: A0A9Q4U0T4
• Organism: Klebsiella pneumoniae strain ST307
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4363100..4373900

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M5S48_RS21415 (M5S48_21415)	gspH	4363256..4363771 (+)	516	WP_032414529.1	type II secretion system minor pseudopilin GspH	-
M5S48_RS21420 (M5S48_21420)	gspI	4363768..4364133 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
M5S48_RS21425 (M5S48_21425)	gspJ	4364130..4364726 (+)	597	WP_032411156.1	type II secretion system minor pseudopilin GspJ	-
M5S48_RS21430 (M5S48_21430)	gspK	4364723..4365709 (+)	987	WP_077254116.1	type II secretion system minor pseudopilin GspK	-
M5S48_RS21435 (M5S48_21435)	gspL	4365696..4366889 (+)	1194	WP_032434208.1	type II secretion system protein GspL	-
M5S48_RS21440 (M5S48_21440)	-	4366882..4367367 (+)	486	WP_004145896.1	type II secretion system protein M	-
M5S48_RS21445 (M5S48_21445)	-	4367357..4368103 (+)	747	WP_032434210.1	type II secretion system protein N	-
M5S48_RS21450 (M5S48_21450)	pilD	4368100..4368900 (+)	801	WP_004213441.1	A24 family peptidase	Machinery gene
M5S48_RS21455 (M5S48_21455)	hrpB	4368948..4371377 (-)	2430	WP_032434212.1	ATP-dependent helicase HrpB	-
M5S48_RS21460 (M5S48_21460)	thpR	4371450..4371986 (+)	537	WP_004177389.1	RNA 2',3'-cyclic phosphodiesterase	-
M5S48_RS21465 (M5S48_21465)	sfsA	4371986..4372702 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
M5S48_RS21470 (M5S48_21470)	dksA	4372865..4373320 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29405.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=724132 M5S48_RS21450 WP_004213441.1 4368100..4368900(+) (pilD) [Klebsiella pneumoniae strain ST307]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPISWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=724132 M5S48_RS21450 WP_004213441.1 4368100..4368900(+) (pilD) [Klebsiella pneumoniae strain ST307]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCTCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGAGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425