Detailed information NTG0723754

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: M5S60_RS20850
• Genome accession: NZ_CP103621
• Coordinates: 4270793..4271593 (+)
• Length: 266 a.a.
• NCBI ID: WP_004191960.1
• Uniprot ID: A0AAJ5B1F9
• Organism: Klebsiella pneumoniae strain 433
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4265793..4276593

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M5S60_RS20815 (M5S60_20815)	gspH	4265949..4266464 (+)	516	WP_062920974.1	type II secretion system minor pseudopilin GspH	-
M5S60_RS20820 (M5S60_20820)	gspI	4266461..4266826 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
M5S60_RS20825 (M5S60_20825)	gspJ	4266823..4267419 (+)	597	WP_064162459.1	type II secretion system minor pseudopilin GspJ	-
M5S60_RS20830 (M5S60_20830)	gspK	4267416..4268402 (+)	987	WP_077265688.1	type II secretion system minor pseudopilin GspK	-
M5S60_RS20835 (M5S60_20835)	gspL	4268389..4269582 (+)	1194	WP_004191957.1	type II secretion system protein GspL	-
M5S60_RS20840 (M5S60_20840)	-	4269575..4270060 (+)	486	WP_004145896.1	type II secretion system protein M	-
M5S60_RS20845 (M5S60_20845)	-	4270050..4270796 (+)	747	WP_023282189.1	type II secretion system protein N	-
M5S60_RS20850 (M5S60_20850)	pilD	4270793..4271593 (+)	801	WP_004191960.1	A24 family peptidase	Machinery gene
M5S60_RS20855 (M5S60_20855)	hrpB	4271641..4274070 (-)	2430	WP_064162458.1	ATP-dependent helicase HrpB	-
M5S60_RS20860 (M5S60_20860)	thpR	4274143..4274679 (+)	537	WP_004177389.1	RNA 2',3'-cyclic phosphodiesterase	-
M5S60_RS20865 (M5S60_20865)	sfsA	4274679..4275395 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
M5S60_RS20870 (M5S60_20870)	dksA	4275558..4276013 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29403.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=723754 M5S60_RS20850 WP_004191960.1 4270793..4271593(+) (pilD) [Klebsiella pneumoniae strain 433]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMI

Nucleotide

• Length: 801 bp

>NTDB_id=723754 M5S60_RS20850 WP_004191960.1 4270793..4271593(+) (pilD) [Klebsiella pneumoniae strain 433]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGATCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.057	99.624	0.489
pilD	Vibrio campbellii strain DS40M4	46.545	100	0.481
pilD	Acinetobacter nosocomialis M2	46.388	98.872	0.459
pilD	Acinetobacter baumannii D1279779	45.627	98.872	0.451
pilD	Neisseria gonorrhoeae MS11	43.922	95.865	0.421