Detailed information NTG0723639

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: M5S85_RS20710
• Genome accession: NZ_CP103605
• Coordinates: 4261810..4262610 (+)
• Length: 266 a.a.
• NCBI ID: WP_021441214.1
• Uniprot ID: A0A4V0HF22
• Organism: Klebsiella pneumoniae strain 4386
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4256810..4267610

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M5S85_RS20675 (M5S85_20675)	gspH	4256966..4257475 (+)	510	WP_021441218.1	type II secretion system minor pseudopilin GspH	-
M5S85_RS20680 (M5S85_20680)	gspI	4257478..4257843 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
M5S85_RS20685 (M5S85_20685)	gspJ	4257840..4258436 (+)	597	WP_032428541.1	type II secretion system minor pseudopilin GspJ	-
M5S85_RS20690 (M5S85_20690)	gspK	4258433..4259419 (+)	987	WP_004183041.1	type II secretion system minor pseudopilin GspK	-
M5S85_RS20695 (M5S85_20695)	gspL	4259406..4260599 (+)	1194	WP_021441216.1	type II secretion system protein GspL	-
M5S85_RS20700 (M5S85_20700)	-	4260592..4261077 (+)	486	WP_004151943.1	type II secretion system protein M	-
M5S85_RS20705 (M5S85_20705)	-	4261067..4261813 (+)	747	WP_048290260.1	type II secretion system protein N	-
M5S85_RS20710 (M5S85_20710)	pilD	4261810..4262610 (+)	801	WP_021441214.1	A24 family peptidase	Machinery gene
M5S85_RS20715 (M5S85_20715)	hrpB	4262658..4265087 (-)	2430	WP_048290259.1	ATP-dependent helicase HrpB	-
M5S85_RS20720 (M5S85_20720)	thpR	4265160..4265696 (+)	537	WP_259384678.1	RNA 2',3'-cyclic phosphodiesterase	-
M5S85_RS20725 (M5S85_20725)	sfsA	4265696..4266412 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
M5S85_RS20730 (M5S85_20730)	dksA	4266575..4267030 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29419.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=723639 M5S85_RS20710 WP_021441214.1 4261810..4262610(+) (pilD) [Klebsiella pneumoniae strain 4386]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
SRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=723639 M5S85_RS20710 WP_021441214.1 4261810..4262610(+) (pilD) [Klebsiella pneumoniae strain 4386]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
AGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
TCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (123-231)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

3D structure

• AlphaFold DB: A0A4V0HF22

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425