Detailed information NTG0723522

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: M5S97_RS21385
• Genome accession: NZ_CP103582
• Coordinates: 4346325..4347125 (+)
• Length: 266 a.a.
• NCBI ID: WP_004145898.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain 1498
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4341325..4352125

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M5S97_RS21350 (M5S97_21350)	gspH	4341481..4341996 (+)	516	WP_032422016.1	type II secretion system minor pseudopilin GspH	-
M5S97_RS21355 (M5S97_21355)	gspI	4341993..4342358 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
M5S97_RS21360 (M5S97_21360)	gspJ	4342355..4342951 (+)	597	WP_023342989.1	type II secretion system minor pseudopilin GspJ	-
M5S97_RS21365 (M5S97_21365)	gspK	4342948..4343934 (+)	987	WP_141408145.1	type II secretion system minor pseudopilin GspK	-
M5S97_RS21370 (M5S97_21370)	gspL	4343921..4345114 (+)	1194	WP_046043759.1	type II secretion system protein GspL	-
M5S97_RS21375 (M5S97_21375)	-	4345107..4345592 (+)	486	WP_004151943.1	type II secretion system protein M	-
M5S97_RS21380 (M5S97_21380)	-	4345582..4346328 (+)	747	WP_021441215.1	type II secretion system protein N	-
M5S97_RS21385 (M5S97_21385)	pilD	4346325..4347125 (+)	801	WP_004145898.1	A24 family peptidase	Machinery gene
M5S97_RS21390 (M5S97_21390)	hrpB	4347173..4349602 (-)	2430	WP_023282188.1	ATP-dependent helicase HrpB	-
M5S97_RS21395 (M5S97_21395)	thpR	4349675..4350211 (+)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
M5S97_RS21400 (M5S97_21400)	sfsA	4350211..4350927 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
M5S97_RS21405 (M5S97_21405)	dksA	4351090..4351545 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29389.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=723522 M5S97_RS21385 WP_004145898.1 4346325..4347125(+) (pilD) [Klebsiella pneumoniae strain 1498]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=723522 M5S97_RS21385 WP_004145898.1 4346325..4347125(+) (pilD) [Klebsiella pneumoniae strain 1498]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
TCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425