Detailed information NTG0723498

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: M5S61_RS21215
• Genome accession: NZ_CP103579
• Coordinates: 4306216..4307016 (+)
• Length: 266 a.a.
• NCBI ID: WP_071081184.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain 4014
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4301216..4312016

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M5S61_RS21180 (M5S61_21180)	gspH	4301372..4301887 (+)	516	WP_004191951.1	type II secretion system minor pseudopilin GspH	-
M5S61_RS21185 (M5S61_21185)	gspI	4301884..4302249 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
M5S61_RS21190 (M5S61_21190)	gspJ	4302246..4302842 (+)	597	WP_071081183.1	type II secretion system minor pseudopilin GspJ	-
M5S61_RS21195 (M5S61_21195)	gspK	4302839..4303825 (+)	987	WP_077270338.1	type II secretion system minor pseudopilin GspK	-
M5S61_RS21200 (M5S61_21200)	gspL	4303812..4305005 (+)	1194	WP_004191957.1	type II secretion system protein GspL	-
M5S61_RS21205 (M5S61_21205)	-	4304998..4305483 (+)	486	WP_004151943.1	type II secretion system protein M	-
M5S61_RS21210 (M5S61_21210)	-	4305473..4306219 (+)	747	WP_023316460.1	type II secretion system protein N	-
M5S61_RS21215 (M5S61_21215)	pilD	4306216..4307016 (+)	801	WP_071081184.1	A24 family peptidase	Machinery gene
M5S61_RS21220 (M5S61_21220)	hrpB	4307064..4309493 (-)	2430	WP_023282188.1	ATP-dependent helicase HrpB	-
M5S61_RS21225 (M5S61_21225)	thpR	4309566..4310102 (+)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
M5S61_RS21230 (M5S61_21230)	sfsA	4310102..4310818 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
M5S61_RS21235 (M5S61_21235)	dksA	4310981..4311436 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29403.98 Da
• Isoelectric Point: 8.8894

>NTDB_id=723498 M5S61_RS21215 WP_071081184.1 4306216..4307016(+) (pilD) [Klebsiella pneumoniae strain 4014]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQQLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=723498 M5S61_RS21215 WP_071081184.1 4306216..4307016(+) (pilD) [Klebsiella pneumoniae strain 4014]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCAGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (123-231)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.057	99.624	0.489
pilD	Vibrio campbellii strain DS40M4	45.818	100	0.474
pilD	Acinetobacter nosocomialis M2	45.627	98.872	0.451
pilD	Acinetobacter baumannii D1279779	44.867	98.872	0.444
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425