Detailed information NTG0723337

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: M5R35_RS23080
• Genome accession: NZ_CP103554
• Coordinates: 4882971..4883768 (+)
• Length: 265 a.a.
• NCBI ID: WP_014227970.1
• Uniprot ID: A0A0H3H8P5
• Organism: Klebsiella michiganensis strain 5088
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4877971..4888768

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M5R35_RS23045 (M5R35_23045)	gspH	4878112..4878624 (+)	513	WP_014227977.1	type II secretion system minor pseudopilin GspH	-
M5R35_RS23050 (M5R35_23050)	gspI	4878621..4878986 (+)	366	WP_014227976.1	type II secretion system minor pseudopilin GspI	-
M5R35_RS23055 (M5R35_23055)	gspJ	4878983..4879579 (+)	597	WP_014227975.1	type II secretion system minor pseudopilin GspJ	-
M5R35_RS23060 (M5R35_23060)	gspK	4879576..4880556 (+)	981	WP_014227974.1	type II secretion system minor pseudopilin GspK	-
M5R35_RS23065 (M5R35_23065)	gspL	4880549..4881742 (+)	1194	WP_014227973.1	type II secretion system protein GspL	-
M5R35_RS23070 (M5R35_23070)	gspM	4881735..4882220 (+)	486	WP_014227972.1	type II secretion system protein GspM	-
M5R35_RS23075 (M5R35_23075)	-	4882210..4882968 (+)	759	WP_038423352.1	type II secretion system protein N	-
M5R35_RS23080 (M5R35_23080)	pilD	4882971..4883768 (+)	798	WP_014227970.1	A24 family peptidase	Machinery gene
M5R35_RS23085 (M5R35_23085)	hrpB	4883866..4886295 (-)	2430	WP_014227969.1	ATP-dependent helicase HrpB	-
M5R35_RS23090 (M5R35_23090)	thpR	4886368..4886904 (+)	537	WP_014227968.1	RNA 2',3'-cyclic phosphodiesterase	-
M5R35_RS23095 (M5R35_23095)	sfsA	4886904..4887620 (+)	717	WP_014227967.1	DNA/RNA nuclease SfsA	-
M5R35_RS23100 (M5R35_23100)	dksA	4887785..4888240 (+)	456	WP_004098689.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 265 a.a.
• Molecular weight: 29166.77 Da
• Isoelectric Point: 8.3358

>NTDB_id=723337 M5R35_RS23080 WP_014227970.1 4882971..4883768(+) (pilD) [Klebsiella michiganensis strain 5088]
MENIALLPEFAAQYPSIWGGFLFLSGLAFGSFFNVVIHRLPLMMERAEGVNLCLPASFCPQCRQPIAWRDNIPLLSFLWL
RGRARCCGQPISRRYPLMELTTGALFVLAGYLMAPGMPLLGGLIFVSVLLILAAIDAQTQLLPDRLTLPLLWAGLLFNLS
DTFAPLAEAVIGAMVGYLSLWSVYWVFRLLTGKEALGYGDFKLLAALGAWLGWQPLPQTLLLASASGLVWTLLQRLVTRR
SLEQPLAFGPWLALAGGGIFLWSQV

Nucleotide

• Length: 798 bp

>NTDB_id=723337 M5R35_RS23080 WP_014227970.1 4882971..4883768(+) (pilD) [Klebsiella michiganensis strain 5088]
ATGGAAAATATCGCGCTGTTACCGGAGTTCGCCGCGCAATACCCGTCAATCTGGGGCGGCTTTTTATTCCTGTCCGGACT
GGCGTTCGGCAGCTTTTTTAACGTCGTTATCCATCGTTTACCGCTGATGATGGAACGCGCAGAGGGGGTGAATCTCTGCC
TCCCCGCGTCGTTCTGCCCGCAGTGCCGTCAGCCTATCGCCTGGCGGGACAATATCCCGCTGCTGAGCTTTTTATGGCTC
AGGGGGCGCGCGCGCTGCTGCGGGCAGCCGATCTCGCGTCGCTATCCCCTGATGGAGCTGACGACCGGCGCGCTGTTTGT
ACTCGCGGGGTACCTGATGGCTCCGGGCATGCCGCTGCTGGGCGGGCTAATTTTCGTGTCCGTACTGCTGATCCTCGCCG
CCATCGACGCGCAAACGCAGCTGCTGCCTGACAGGCTGACGCTGCCGCTGCTGTGGGCCGGGCTGCTGTTCAATCTTTCC
GATACCTTCGCGCCGCTGGCGGAGGCGGTCATTGGCGCGATGGTCGGGTATTTGTCGCTGTGGTCGGTGTACTGGGTGTT
CCGTCTGCTGACCGGTAAAGAGGCGCTGGGCTACGGCGATTTCAAGCTGCTGGCAGCGCTCGGCGCGTGGCTCGGCTGGC
AGCCGCTGCCGCAGACGCTGCTGCTGGCTTCCGCCAGCGGTCTGGTCTGGACGCTGCTGCAGCGTCTGGTAACCCGTCGT
TCGCTCGAACAACCTCTGGCCTTCGGCCCCTGGCTGGCGCTGGCGGGCGGCGGCATCTTTTTGTGGAGCCAGGTATAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (25-110)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

3D structure

• AlphaFold DB: A0A0H3H8P5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	48.708	100	0.498
pilD	Neisseria gonorrhoeae MS11	44.604	100	0.468
pilD	Vibrio campbellii strain DS40M4	43.636	100	0.453
pilD	Acinetobacter nosocomialis M2	44.788	97.736	0.438
pilD	Acinetobacter baumannii D1279779	44.402	97.736	0.434