Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   NX050_RS03100 Genome accession   NZ_CP103456
Coordinates   600968..602359 (+) Length   463 a.a.
NCBI ID   WP_014481052.1    Uniprot ID   -
Organism   Bacillus subtilis strain PN176 (HK176)     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 595968..607359
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NX050_RS03075 (NX050_03075) - 596127..597119 (-) 993 WP_014481056.1 LCP family protein -
  NX050_RS03080 (NX050_03080) - 597119..597772 (-) 654 WP_014481055.1 YigZ family protein -
  NX050_RS03085 (NX050_03085) degS 597989..599146 (+) 1158 WP_003227983.1 two-component sensor histidine kinase DegS Regulator
  NX050_RS03090 (NX050_03090) degU 599229..599918 (+) 690 WP_014481054.1 two-component system response regulator DegU Regulator
  NX050_RS03095 (NX050_03095) fakBA 600016..600861 (+) 846 WP_014481053.1 DegV family protein -
  NX050_RS03100 (NX050_03100) comFA 600968..602359 (+) 1392 WP_014481052.1 ATP-dependent helicase ComFA Machinery gene
  NX050_RS03105 (NX050_03105) comFB 602419..602715 (+) 297 WP_003227989.1 late competence protein ComFB -
  NX050_RS03110 (NX050_03110) comFC 602679..603401 (+) 723 WP_014481051.1 comF operon protein ComFC Machinery gene
  NX050_RS03115 (NX050_03115) yvyF 603474..603893 (+) 420 WP_014481050.1 TIGR03826 family flagellar region protein -
  NX050_RS03120 (NX050_03120) flgM 603974..604240 (+) 267 WP_014481049.1 flagellar biosynthesis anti-sigma factor FlgM -
  NX050_RS03125 (NX050_03125) flgN 604256..604738 (+) 483 WP_014481048.1 flagellar protein FlgN -
  NX050_RS03130 (NX050_03130) flgK 604757..606280 (+) 1524 WP_014481047.1 flagellar hook-associated protein FlgK -
  NX050_RS03135 (NX050_03135) flgL 606291..607187 (+) 897 WP_014481046.1 flagellar hook-associated protein FlgL -

Sequence

Protein


Download         Length: 463 a.a.        Molecular weight: 52543.49 Da        Isoelectric Point: 9.7976

>NTDB_id=722854 NX050_RS03100 WP_014481052.1 600968..602359(+) (comFA) [Bacillus subtilis strain PN176 (HK176)]
MNVPVEKNSSFSRELQQTLRSRHLLRTELSFSDEMIEWHIKNGYITAENSISINKRGYRCNRCGQTDQRYYSFYHSSGKN
KLYCRSCVMMGRVSEEVPLYSWEEENEPNWQSIKLTWDGKLSSGQQKAANVLIEAISKKEELLIWAVCGAGKTEMLFPGI
ESALNQGLRVCIATPRTDVVLELAPRLKAAFQGADISALYGGSDDKGRLSPLMISTTHQLLRYKDAIDVMIIDEVDAFPY
SADQTLQFAVQKARKKNSTLVYLSATPPKELKRKALNGQLHSVRIPARHHRKPLPEPRFVWCGNWKKKLNRNKIPPAVKR
WIEFHVKEGRPVFLFVPSVSILEKAAACFRGVHCRTASVHAEDKHRKEKVQQFRDGQLDLLITTTILERGVTVPKVQTGV
LGAESPIFTESALVQIAGRTGRHKEYADGDVIFFHFGKTKSMLDARKHIKEMNELAAKVECTD

Nucleotide


Download         Length: 1392 bp        

>NTDB_id=722854 NX050_RS03100 WP_014481052.1 600968..602359(+) (comFA) [Bacillus subtilis strain PN176 (HK176)]
GTGAATGTGCCAGTTGAAAAAAACAGTTCCTTTTCTAGAGAATTGCAGCAGACGCTTCGAAGCCGTCATTTGCTCAGGAC
TGAGCTCTCATTTTCCGATGAGATGATTGAATGGCATATCAAGAATGGATATATCACTGCTGAAAATTCTATATCCATAA
ATAAACGGGGATATAGATGTAATAGGTGCGGCCAAACTGATCAGCGGTATTATTCTTTTTATCACTCATCTGGAAAGAAT
AAGCTGTATTGCCGTTCCTGTGTCATGATGGGCAGAGTGAGTGAGGAGGTTCCTTTATATTCATGGGAAGAGGAAAATGA
ACCAAACTGGCAGTCAATTAAACTGACATGGGACGGCAAGCTTTCAAGCGGACAGCAAAAAGCCGCCAATGTTTTAATTG
AAGCAATATCAAAAAAAGAAGAGCTCCTCATCTGGGCGGTTTGCGGCGCTGGCAAAACAGAAATGCTGTTTCCTGGTATA
GAATCAGCGTTAAATCAAGGACTGCGTGTATGTATTGCAACACCTCGCACCGATGTTGTATTAGAGCTTGCTCCAAGGCT
CAAGGCTGCCTTTCAGGGGGCTGACATTTCAGCGCTTTACGGAGGAAGCGATGACAAAGGGCGGCTATCTCCGCTTATGA
TTTCCACTACGCATCAGCTTTTGCGATATAAAGATGCAATCGATGTTATGATCATTGATGAAGTTGACGCTTTTCCATAT
TCCGCTGATCAAACCCTTCAATTCGCTGTTCAAAAAGCAAGAAAGAAAAACAGCACCCTCGTTTATTTAAGTGCAACACC
TCCTAAAGAATTAAAAAGAAAAGCACTGAACGGACAGTTACATTCAGTTCGCATCCCCGCAAGACACCACCGGAAACCTT
TACCCGAACCGCGCTTTGTATGGTGTGGAAACTGGAAGAAGAAATTAAACCGAAATAAAATTCCGCCAGCGGTGAAAAGA
TGGATAGAGTTTCATGTAAAAGAAGGGAGGCCTGTTTTTTTATTCGTTCCTTCCGTTTCTATTCTGGAAAAGGCTGCTGC
GTGCTTTAGAGGTGTTCATTGCCGAACCGCATCTGTGCACGCGGAAGACAAGCATAGAAAGGAGAAAGTGCAGCAATTCA
GAGATGGTCAGCTCGATCTATTAATCACAACAACAATACTGGAAAGAGGCGTCACAGTCCCCAAGGTGCAAACGGGTGTA
CTAGGAGCGGAATCACCTATCTTTACGGAAAGCGCACTTGTTCAAATTGCAGGAAGAACCGGCCGGCATAAAGAATATGC
GGACGGCGATGTCATTTTCTTTCACTTCGGCAAAACAAAGAGTATGCTCGATGCAAGAAAGCATATAAAAGAAATGAATG
AATTGGCAGCAAAAGTTGAATGTACAGACTAG

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(120-268)

Helicase conserved C-terminal domain (Helicase_C)

(326-423)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

98.056

100

0.981