Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   NX856_RS13645 Genome accession   NZ_CP103412
Coordinates   2714894..2715964 (-) Length   356 a.a.
NCBI ID   WP_124692840.1    Uniprot ID   -
Organism   Bacillus velezensis strain HF-14109     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2709894..2720964
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NX856_RS13595 (NX856_13595) sipW 2709954..2710538 (-) 585 WP_015240205.1 signal peptidase I SipW -
  NX856_RS13600 (NX856_13600) tapA 2710510..2711181 (-) 672 WP_124692843.1 amyloid fiber anchoring/assembly protein TapA -
  NX856_RS13605 (NX856_13605) - 2711440..2711769 (+) 330 WP_012117979.1 DUF3889 domain-containing protein -
  NX856_RS13610 (NX856_13610) - 2711809..2711988 (-) 180 WP_003153093.1 YqzE family protein -
  NX856_RS13615 (NX856_13615) comGG 2712045..2712422 (-) 378 WP_015240208.1 competence type IV pilus minor pilin ComGG Machinery gene
  NX856_RS13620 (NX856_13620) comGF 2712423..2712923 (-) 501 WP_256994853.1 competence type IV pilus minor pilin ComGF -
  NX856_RS13625 (NX856_13625) comGE 2712832..2713146 (-) 315 WP_012117982.1 competence type IV pilus minor pilin ComGE -
  NX856_RS13630 (NX856_13630) comGD 2713130..2713567 (-) 438 WP_015240210.1 competence type IV pilus minor pilin ComGD Machinery gene
  NX856_RS13635 (NX856_13635) comGC 2713557..2713823 (-) 267 WP_042635730.1 competence type IV pilus major pilin ComGC Machinery gene
  NX856_RS13640 (NX856_13640) comGB 2713870..2714907 (-) 1038 WP_218791295.1 competence type IV pilus assembly protein ComGB Machinery gene
  NX856_RS13645 (NX856_13645) comGA 2714894..2715964 (-) 1071 WP_124692840.1 competence type IV pilus ATPase ComGA Machinery gene
  NX856_RS13650 (NX856_13650) - 2716157..2717107 (-) 951 WP_007408319.1 magnesium transporter CorA family protein -
  NX856_RS13655 (NX856_13655) - 2717253..2718554 (+) 1302 WP_012117986.1 hemolysin family protein -
  NX856_RS13660 (NX856_13660) - 2718592..2719422 (-) 831 WP_007408317.1 STAS domain-containing protein -
  NX856_RS13670 (NX856_13670) - 2719629..2720009 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  NX856_RS13675 (NX856_13675) - 2720247..2720492 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40529.85 Da        Isoelectric Point: 9.4729

>NTDB_id=722668 NX856_RS13645 WP_124692840.1 2714894..2715964(-) (comGA) [Bacillus velezensis strain HF-14109]
MENIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGK
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=722668 NX856_RS13645 WP_124692840.1 2714894..2715964(-) (comGA) [Bacillus velezensis strain HF-14109]
GTGGAAAATATAGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTAATTTCTCATTTTAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAATTGTCCTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGTCTGCTTCTGTTTACCGGCCCCACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGCTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTCGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAAAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATTGCACTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.022

100

0.82
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.123

95.787

0.365