Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   NX856_RS13640 Genome accession   NZ_CP103412
Coordinates   2713870..2714907 (-) Length   345 a.a.
NCBI ID   WP_218791295.1    Uniprot ID   -
Organism   Bacillus velezensis strain HF-14109     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2708870..2719907
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NX856_RS13590 (NX856_13590) tasA 2709104..2709889 (-) 786 WP_007408329.1 biofilm matrix protein TasA -
  NX856_RS13595 (NX856_13595) sipW 2709954..2710538 (-) 585 WP_015240205.1 signal peptidase I SipW -
  NX856_RS13600 (NX856_13600) tapA 2710510..2711181 (-) 672 WP_124692843.1 amyloid fiber anchoring/assembly protein TapA -
  NX856_RS13605 (NX856_13605) - 2711440..2711769 (+) 330 WP_012117979.1 DUF3889 domain-containing protein -
  NX856_RS13610 (NX856_13610) - 2711809..2711988 (-) 180 WP_003153093.1 YqzE family protein -
  NX856_RS13615 (NX856_13615) comGG 2712045..2712422 (-) 378 WP_015240208.1 competence type IV pilus minor pilin ComGG Machinery gene
  NX856_RS13620 (NX856_13620) comGF 2712423..2712923 (-) 501 WP_256994853.1 competence type IV pilus minor pilin ComGF -
  NX856_RS13625 (NX856_13625) comGE 2712832..2713146 (-) 315 WP_012117982.1 competence type IV pilus minor pilin ComGE -
  NX856_RS13630 (NX856_13630) comGD 2713130..2713567 (-) 438 WP_015240210.1 competence type IV pilus minor pilin ComGD Machinery gene
  NX856_RS13635 (NX856_13635) comGC 2713557..2713823 (-) 267 WP_042635730.1 competence type IV pilus major pilin ComGC Machinery gene
  NX856_RS13640 (NX856_13640) comGB 2713870..2714907 (-) 1038 WP_218791295.1 competence type IV pilus assembly protein ComGB Machinery gene
  NX856_RS13645 (NX856_13645) comGA 2714894..2715964 (-) 1071 WP_124692840.1 competence type IV pilus ATPase ComGA Machinery gene
  NX856_RS13650 (NX856_13650) - 2716157..2717107 (-) 951 WP_007408319.1 magnesium transporter CorA family protein -
  NX856_RS13655 (NX856_13655) - 2717253..2718554 (+) 1302 WP_012117986.1 hemolysin family protein -
  NX856_RS13660 (NX856_13660) - 2718592..2719422 (-) 831 WP_007408317.1 STAS domain-containing protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 39501.78 Da        Isoelectric Point: 9.5935

>NTDB_id=722667 NX856_RS13640 WP_218791295.1 2713870..2714907(-) (comGB) [Bacillus velezensis strain HF-14109]
MKRIKRTWPLKDQAAFLKRLGEMTEKGYSLIEGLRLLKLQLHKRQLAELTDGIRRLREGDAFYQVLEALSFHKEAVSICY
FAEKHGELPGAMKQSGDLLQRKLMQTNQIKKMLRYPMFLISSVCVMFYILQTLIIPQFSGIYQSMNMNTSGATAFIFAFF
GHFHEACALALSAAFCLFLYVWFLCKKKSPQDKMLIVVKIPLLGKAAVLFNSYFFSLQLSSLLKSGLSIYDSLTAFKEQS
FLPFYREEAEMLITRLKAGETIESALSGHPCYEKDLAAAVSHGQANGRLHRELYTYSQFMMERLEQNAAKYTGILQPVIY
GVVAGMILIVYMSMLMPMYQMMNQM

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=722667 NX856_RS13640 WP_218791295.1 2713870..2714907(-) (comGB) [Bacillus velezensis strain HF-14109]
ATGAAGCGGATTAAACGAACCTGGCCCTTGAAGGATCAAGCGGCTTTTTTGAAAAGGCTCGGTGAAATGACAGAAAAAGG
ATACAGTCTGATTGAAGGGCTCAGGCTGCTGAAACTCCAGCTGCATAAACGTCAGCTTGCAGAGCTGACGGACGGAATCC
GCCGGCTGAGGGAAGGTGATGCGTTTTACCAAGTGCTTGAGGCACTGTCTTTTCACAAAGAAGCCGTCTCTATCTGCTAT
TTCGCGGAGAAACACGGGGAATTGCCGGGGGCGATGAAACAAAGCGGCGACTTGTTACAGAGAAAGCTTATGCAGACGAA
TCAAATCAAAAAAATGCTCCGGTATCCGATGTTTTTAATATCTTCCGTCTGCGTCATGTTTTATATCCTGCAAACTTTAA
TTATTCCGCAGTTTTCTGGGATTTATCAATCAATGAATATGAATACATCCGGTGCGACTGCTTTCATCTTTGCATTTTTT
GGGCATTTTCATGAAGCCTGCGCACTGGCGCTGTCCGCCGCTTTCTGCTTGTTTTTGTATGTTTGGTTCTTATGTAAGAA
AAAATCCCCGCAAGATAAAATGCTGATTGTTGTGAAAATACCCCTCTTGGGAAAAGCGGCCGTTCTTTTTAACAGCTACT
TTTTCTCTTTACAGCTCAGCAGTCTTCTGAAATCCGGCCTATCTATCTATGACAGCTTAACAGCTTTTAAAGAACAATCT
TTTCTGCCTTTTTATCGTGAAGAAGCAGAGATGCTCATTACACGTCTGAAAGCCGGAGAAACCATAGAATCCGCTCTTTC
CGGGCATCCGTGTTATGAAAAAGACCTTGCGGCTGCAGTCAGCCACGGACAGGCAAACGGCCGGCTTCACCGCGAACTTT
ATACGTACAGCCAGTTTATGATGGAGCGTCTTGAACAAAACGCGGCGAAGTATACCGGCATTCTCCAGCCTGTCATTTAC
GGTGTTGTGGCCGGTATGATTTTAATTGTCTACATGTCAATGCTGATGCCGATGTACCAGATGATGAACCAAATGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(215-337)

Type II secretion system (T2SS), protein F (T2SSF)

(16-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

65.635

93.623

0.614