Detailed information    

insolico Bioinformatically predicted

Overview

Name   recF   Type   Machinery gene
Locus tag   PWK65_RS09355 Genome accession   NZ_CP118481
Coordinates   1869443..1870549 (+) Length   368 a.a.
NCBI ID   WP_002991449.1    Uniprot ID   Q48QL2
Organism   Streptococcus pyogenes strain 20185322     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1864443..1875549
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWK65_RS09335 (PWK65_09260) hasA 1865121..1866380 (+) 1260 WP_002991440.1 hyaluronan synthase HasA -
  PWK65_RS09340 (PWK65_09265) hasB 1866416..1867624 (+) 1209 WP_002991442.1 UDP-glucose 6-dehydrogenase HasB -
  PWK65_RS09345 (PWK65_09270) hasC 1867806..1868720 (+) 915 WP_002991444.1 UTP--glucose-1-phosphate uridylyltransferase HasC -
  PWK65_RS09350 (PWK65_09275) yaaA 1869028..1869441 (+) 414 WP_002991446.1 S4 domain-containing protein YaaA -
  PWK65_RS09355 (PWK65_09280) recF 1869443..1870549 (+) 1107 WP_002991449.1 DNA replication/repair protein RecF Machinery gene
  PWK65_RS09360 (PWK65_09285) - 1870606..1871469 (-) 864 WP_002991450.1 GRP family sugar transporter -
  PWK65_RS09365 (PWK65_09290) guaB 1871672..1873153 (-) 1482 WP_002991454.1 IMP dehydrogenase -
  PWK65_RS09370 (PWK65_09295) trpS 1873461..1874483 (-) 1023 WP_002991455.1 tryptophan--tRNA ligase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41780.70 Da        Isoelectric Point: 8.4064

>NTDB_id=722526 PWK65_RS09355 WP_002991449.1 1869443..1870549(+) (recF) [Streptococcus pyogenes strain 20185322]
MWIKELELKHYRNYDHLLASFSSGLNVFIGNNAQGKTNFLEAIYFLSLTRSHRTRADKELIHFDHSTVSLTGKIQRISGT
VDLEINLSDKGRVTKINALKQAKLSDYIGTMMVVLFAPEDLQLVKGAPSLRRKFIDIDLGQIKPVYLSELSHYNHVLKQR
NSYLKSAQQIDAAFLAVLDEQLAGYGARVMEHRIDFINALEKEANTHHQAISNGLESLSLSYQSSVVFDKKTNIYQQFLH
QLEKNHQKDFFRKNTSVGPHRDNLAFYINGMNANFASQGQHRSLILSLKMAEVSLMKALTGDNPILLLDDVMSELDNTRQ
TKLLETVIKENVQTFITTTSLDHLSQLPEGIRIFHVTKGTVQVDSDIH

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=722526 PWK65_RS09355 WP_002991449.1 1869443..1870549(+) (recF) [Streptococcus pyogenes strain 20185322]
ATGTGGATTAAAGAGTTAGAGTTAAAACATTATCGCAATTATGACCACCTGCTTGCCTCATTTTCGTCGGGTTTAAATGT
CTTTATCGGTAATAATGCTCAAGGAAAAACCAATTTTCTCGAGGCAATCTATTTTCTATCACTTACTAGAAGCCATCGCA
CGAGGGCTGATAAAGAATTGATTCATTTTGATCACTCAACTGTTTCTCTTACTGGTAAGATTCAGCGTATTAGTGGTACT
GTTGATTTAGAAATCAATCTATCAGATAAAGGACGTGTGACAAAGATTAATGCGCTAAAGCAAGCAAAATTATCTGATTA
TATTGGAACGATGATGGTTGTGCTTTTTGCACCAGAAGATTTGCAACTAGTTAAGGGAGCACCTAGCCTTCGTCGCAAAT
TTATCGACATTGACCTTGGTCAAATTAAGCCTGTTTACTTATCTGAGTTATCCCATTATAACCACGTGCTTAAACAGCGT
AATAGCTATTTAAAAAGTGCCCAACAAATTGATGCCGCTTTTCTAGCTGTTCTAGATGAGCAATTAGCTGGCTATGGAGC
CCGTGTCATGGAACATCGTATTGATTTTATCAATGCGTTAGAGAAAGAAGCAAATACTCATCACCAAGCCATCTCAAATG
GTTTAGAAAGTCTCTCTCTCTCCTATCAGTCGTCAGTTGTCTTTGACAAAAAAACTAATATTTACCAACAATTTTTACAC
CAACTTGAAAAAAATCATCAGAAAGATTTCTTCCGTAAAAACACTAGCGTTGGTCCACATCGCGATAACTTAGCCTTTTA
CATTAACGGGATGAATGCTAACTTTGCTAGTCAAGGTCAGCACCGCAGCCTTATTCTTTCCTTAAAAATGGCTGAAGTTA
GTTTAATGAAGGCCTTGACTGGAGATAACCCCATTCTTTTACTAGACGATGTCATGAGCGAGCTAGACAATACACGACAA
ACAAAGTTACTTGAAACAGTAATTAAAGAAAATGTCCAAACCTTTATCACAACAACAAGTTTGGATCACCTGTCACAGTT
GCCAGAAGGTATCCGTATTTTTCACGTAACAAAAGGAACCGTTCAAGTTGATTCTGATATTCACTAA

Domains

Predicted by InterProScan.

RecF/RecN/SMC N terminal domain (SMC_N)

(3-360)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q48QL2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recF Streptococcus pneumoniae R6

62.842

99.457

0.625
  recF Bacillus subtilis subsp. subtilis str. 168

45.455

100

0.462