Detailed information NTG0720527

Bioinformatically predicted

Overview

• Name: hexA
• Type: Machinery gene
• Locus tag: PUW57_RS10280
• Genome accession: NZ_CP118043
• Coordinates: 2009093..2011669 (-)
• Length: 858 a.a.
• NCBI ID: WP_001118377.1
• Uniprot ID: Q8DWW1
• Organism: Streptococcus agalactiae strain VSI63
• Function: DNA mismatch repair (predicted from homology) Homologous recombination

Genomic Context

Location: 2004093..2016669

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PUW57_RS10255 (PUW57_10255)	-	2004204..2004755 (-)	552	WP_000817341.1	DNA-3-methyladenine glycosylase I	-
PUW57_RS10260 (PUW57_10260)	ruvA	2004778..2005368 (-)	591	WP_000272487.1	Holliday junction branch migration protein RuvA	Machinery gene
PUW57_RS10265 (PUW57_10265)	-	2005370..2006602 (-)	1233	WP_000657476.1	MFS transporter	-
PUW57_RS10270 (PUW57_10270)	hexB	2006634..2008613 (-)	1980	WP_001054074.1	DNA mismatch repair endonuclease MutL	Machinery gene
PUW57_RS10275 (PUW57_10275)	-	2008833..2009036 (+)	204	WP_000191849.1	cold shock domain-containing protein	-
PUW57_RS10280 (PUW57_10280)	hexA	2009093..2011669 (-)	2577	WP_001118377.1	DNA mismatch repair protein MutS	Machinery gene
PUW57_RS10285 (PUW57_10285)	argR	2011726..2012163 (-)	438	WP_001033152.1	arginine repressor	-
PUW57_RS10290 (PUW57_10290)	argS	2012385..2014076 (+)	1692	WP_000379868.1	arginine--tRNA ligase	-
PUW57_RS10295 (PUW57_10295)	-	2014164..2014472 (+)	309	WP_001138859.1	bacteriocin immunity protein	-
PUW57_RS10300 (PUW57_10300)	-	2014499..2015371 (-)	873	WP_000915479.1	YitT family protein	-
PUW57_RS10305 (PUW57_10305)	-	2015479..2016423 (-)	945	WP_000857770.1	YitT family protein	-

Sequence

Protein

• Length: 858 a.a.
• Molecular weight: 97078.70 Da
• Isoelectric Point: 4.8140

>NTDB_id=720527 PUW57_RS10280 WP_001118377.1 2009093..2011669(-) (hexA) [Streptococcus agalactiae strain VSI63]
MAKPTISPGMQQYLDIKENYPDAFLLFRMGDFYELFYDDAVKAAQILEISLTSRNKNAEKPIPMAGVPYHSAQQYIDVLV
ELGYKVAIAEQMEDPKKAVGVVKREVVQVVTPGTVVESTKPDSANNFLVAIDSQDQQTFGLAYMDVSTGEFQATLLTDFE
SVRSEILNLKAREIVVGYQLTDEKNHLLTKQMNLLLSYEDERLNDIHLIDEQLTDLEISAAEKLLQYVHRTQKRELSHLQ
KVVHYEIKDYLQMSYATKNSLDLLENARTSKKHGSLYWLLDETKTAMGTRMLRTWIDRPLVSMNRIKERQDIIQVFLDYF
FERNDLTESLKGVYDIERLASRVSFGKANPKDLLQLGQTLSQIPRIKMILQSFNQPELDIIVNKIDTMPELESLINTAIA
PEAQATITEGNIIKSGFDKQLDNYRTVMREGTGWIADIEAKERAASGIGTLKIDYNKKDGYYFHVTNSNLSLVPEHFFRK
ATLKNSERYGTAELAKIEGEMLEAREQSSNLEYDIFMRVRAQVESYIKRLQELAKTIATVDVLQSLAVVAENYHYVRPKF
NDQHQIKIKNGRHATVEKVMGVQEYIPNSIYFDSQTDIQLITGPNMSGKSTYMRQLALTVIMAQMGGFVSADEVDLPVFD
AIFTRIGAADDLISGQSTFMVEMMEANQAVKRASDKSLILFDELGRGTATYDGMALAQSIIEYIHDRVRAKTMFATHYHE
LTDLSEQLTRLVNVHVATLERDGEVTFLHKIESGPADKSYGIHVAKIAGLPIDLLDRATDILSQLEADAVQLIVSPSQEA
VTADLNEELDSEKQQGQLSLFEEPSNAGRVIEELEAIDIMNLTPMQAMNAIFDLKKLL

Nucleotide

• Length: 2577 bp

>NTDB_id=720527 PUW57_RS10280 WP_001118377.1 2009093..2011669(-) (hexA) [Streptococcus agalactiae strain VSI63]
ATGGCCAAGCCAACGATATCACCGGGAATGCAACAGTATCTGGATATAAAAGAGAATTATCCAGATGCTTTTTTGCTTTT
TAGAATGGGTGATTTTTATGAATTATTTTATGATGATGCGGTAAAAGCAGCACAAATCCTGGAAATTAGCTTGACTAGTC
GAAATAAGAACGCAGAAAAGCCAATCCCAATGGCAGGAGTTCCCTATCACTCAGCTCAACAGTATATTGACGTTTTAGTT
GAATTAGGTTACAAAGTAGCCATTGCTGAGCAGATGGAAGATCCTAAAAAAGCTGTGGGAGTGGTCAAGCGTGAGGTAGT
GCAAGTTGTTACCCCAGGAACGGTTGTGGAGTCAACGAAGCCGGATAGTGCTAATAATTTCTTAGTAGCGATTGATTCGC
AAGATCAACAAACATTTGGTCTAGCATATATGGATGTCTCAACTGGAGAGTTTCAGGCAACCCTTTTAACAGATTTTGAG
TCCGTCCGTAGTGAAATACTAAATTTAAAAGCTCGTGAGATTGTAGTAGGATATCAATTGACGGACGAAAAAAATCACCT
ACTGACGAAGCAGATGAACTTGCTTTTATCATACGAAGACGAACGACTTAATGATATTCATTTGATTGATGAGCAGTTAA
CTGATTTGGAAATATCTGCTGCGGAAAAACTTTTACAATATGTGCATAGAACACAAAAGCGTGAACTTAGTCATTTACAG
AAAGTAGTTCATTATGAAATAAAGGACTATTTACAAATGTCATATGCAACGAAAAATAGTCTAGATTTACTGGAGAATGC
TAGAACAAGCAAGAAGCATGGAAGTCTTTACTGGTTGTTAGATGAGACTAAAACAGCGATGGGAACTCGAATGCTGAGAA
CTTGGATTGACAGGCCTTTGGTAAGTATGAATCGAATCAAGGAACGTCAAGATATTATTCAAGTGTTTCTTGATTATTTT
TTTGAGAGAAACGATCTCACAGAAAGTTTAAAGGGTGTATATGATATTGAACGCCTAGCAAGTCGAGTATCTTTCGGAAA
AGCCAACCCTAAAGATCTATTGCAACTCGGACAGACCTTATCACAAATTCCTCGGATTAAAATGATTTTACAGTCCTTCA
ATCAACCTGAGCTTGACATCATTGTCAACAAAATTGACACTATGCCTGAATTAGAAAGTTTAATTAATACGGCGATAGCC
CCAGAAGCACAGGCTACTATCACTGAGGGAAACATTATCAAGTCTGGATTTGATAAGCAATTGGATAATTATCGAACAGT
GATGCGTGAAGGTACAGGTTGGATTGCTGATATTGAAGCTAAGGAAAGAGCAGCAAGTGGTATCGGTACTCTTAAAATTG
ATTATAATAAAAAAGACGGTTATTACTTCCATGTTACCAATTCCAATTTATCACTAGTACCGGAGCATTTTTTCCGTAAA
GCGACATTAAAAAATTCTGAACGCTATGGAACAGCAGAACTAGCCAAAATTGAAGGTGAAATGCTCGAAGCTCGCGAGCA
ATCTTCAAATTTAGAATATGATATTTTTATGCGTGTTCGTGCCCAAGTAGAATCTTATATTAAACGTCTTCAAGAGTTAG
CAAAGACGATTGCAACCGTTGATGTTCTACAGAGTTTGGCAGTAGTTGCAGAAAATTATCACTATGTTCGTCCCAAATTT
AATGATCAACATCAGATTAAGATTAAGAATGGGCGTCATGCAACTGTTGAAAAAGTGATGGGAGTGCAAGAATATATTCC
CAATAGCATCTATTTTGATAGTCAGACAGATATCCAGTTGATTACAGGACCAAATATGAGTGGTAAGTCGACCTATATGC
GCCAGTTAGCTTTGACAGTTATTATGGCACAAATGGGAGGTTTTGTATCGGCAGACGAAGTTGATTTGCCTGTATTTGAT
GCAATATTTACTAGGATTGGTGCTGCTGACGACTTAATTTCTGGGCAATCAACCTTTATGGTAGAAATGATGGAAGCGAA
TCAAGCTGTAAAACGAGCCAGTGATAAATCTTTGATTCTTTTTGATGAATTAGGTCGAGGGACAGCCACTTATGATGGTA
TGGCATTAGCTCAATCGATTATAGAATATATTCATGACCGTGTTAGGGCAAAAACAATGTTTGCGACTCATTACCATGAG
TTGACAGATTTATCTGAACAGTTGACAAGGCTTGTCAATGTACACGTGGCTACTTTAGAGAGAGATGGAGAAGTTACCTT
CTTACATAAAATTGAATCTGGACCTGCGGATAAGTCTTATGGGATACACGTCGCAAAAATAGCTGGTTTACCAATTGACT
TATTGGATAGGGCAACTGATATTTTATCACAGTTGGAAGCTGATGCAGTACAGTTGATCGTATCGCCCTCCCAAGAAGCT
GTTACTGCTGACTTAAATGAGGAACTAGATTCTGAGAAGCAACAAGGACAATTATCGCTTTTTGAAGAACCTTCAAATGC
AGGTAGGGTTATTGAGGAGTTAGAAGCGATAGATATAATGAATCTAACTCCAATGCAAGCTATGAATGCTATATTTGACT
TAAAGAAATTATTATAA

Domains

Predicted by InterProScan.

• MutS domain II (MutS_II) (126-240)

• MutS family domain IV (MutS_IV) (416-506)

• MutS domain III (MutS_III) (257-547)

• MutS domain V (MutS_V) (600-786)

• MutS domain I (MutS_I) (8-118)

3D structure

• AlphaFold DB: Q8DWW1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
hexA	Streptococcus pneumoniae R6	69.114	100	0.691
mutS	Pseudomonas stutzeri strain ATCC 17587	36.519	100	0.374