Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   NW959_RS07300 Genome accession   NZ_CP102957
Coordinates   1540019..1540993 (-) Length   324 a.a.
NCBI ID   WP_000697220.1    Uniprot ID   W8U5L8
Organism   Staphylococcus aureus strain 18-H-62     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1535019..1545993
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NW959_RS07260 (NW959_07260) gcvT 1535520..1536611 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  NW959_RS07265 (NW959_07265) - 1536770..1537294 (-) 525 WP_001015121.1 shikimate kinase -
  NW959_RS07270 (NW959_07270) - 1537284..1537430 (-) 147 WP_001789879.1 hypothetical protein -
  NW959_RS07275 (NW959_07275) comGF 1537527..1538024 (-) 498 WP_029694050.1 competence type IV pilus minor pilin ComGF Machinery gene
  NW959_RS07280 (NW959_07280) comGE 1537942..1538241 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  NW959_RS07285 (NW959_07285) comGD 1538228..1538674 (-) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  NW959_RS07290 (NW959_07290) comGC 1538652..1538963 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  NW959_RS07295 (NW959_07295) comGB 1538977..1540047 (-) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  NW959_RS07300 (NW959_07300) comGA 1540019..1540993 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  NW959_RS07305 (NW959_07305) - 1541045..1541668 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  NW959_RS07310 (NW959_07310) - 1541665..1541993 (-) 329 Protein_1432 MTH1187 family thiamine-binding protein -
  NW959_RS07315 (NW959_07315) - 1541993..1542979 (-) 987 WP_258413885.1 ROK family glucokinase -
  NW959_RS07320 (NW959_07320) - 1542976..1543179 (-) 204 WP_258410696.1 YqgQ family protein -
  NW959_RS07325 (NW959_07325) - 1543160..1544623 (-) 1464 WP_001017867.1 rhomboid family intramembrane serine protease -
  NW959_RS07330 (NW959_07330) - 1544635..1545174 (-) 540 WP_000164546.1 5-formyltetrahydrofolate cyclo-ligase -
  NW959_RS07335 (NW959_07335) rpmG 1545383..1545532 (-) 150 WP_001265709.1 50S ribosomal protein L33 -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36897.96 Da        Isoelectric Point: 8.8516

>NTDB_id=720420 NW959_RS07300 WP_000697220.1 1540019..1540993(-) (comGA) [Staphylococcus aureus strain 18-H-62]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=720420 NW959_RS07300 WP_000697220.1 1540019..1540993(-) (comGA) [Staphylococcus aureus strain 18-H-62]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGTACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGGCCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAAATGCAAATTCCTGGTATCGTCCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W8U5L8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

100

100

1
  comGA Staphylococcus aureus N315

100

100

1