Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   NW967_RS07330 Genome accession   NZ_CP102952
Coordinates   1554893..1555390 (-) Length   165 a.a.
NCBI ID   WP_258411170.1    Uniprot ID   -
Organism   Staphylococcus aureus strain 39-B-49     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1549893..1560390
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NW967_RS07305 (NW967_07305) gcvPB 1550055..1551527 (-) 1473 WP_000202187.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  NW967_RS07310 (NW967_07310) gcvPA 1551520..1552866 (-) 1347 WP_000019691.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  NW967_RS07315 (NW967_07315) gcvT 1552886..1553977 (-) 1092 WP_000093343.1 glycine cleavage system aminomethyltransferase GcvT -
  NW967_RS07320 (NW967_07320) - 1554136..1554660 (-) 525 WP_001015120.1 shikimate kinase -
  NW967_RS07325 (NW967_07325) - 1554650..1554796 (-) 147 WP_001789879.1 hypothetical protein -
  NW967_RS07330 (NW967_07330) comGF 1554893..1555390 (-) 498 WP_258411170.1 competence type IV pilus minor pilin ComGF Machinery gene
  NW967_RS07335 (NW967_07335) comGE 1555308..1555607 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  NW967_RS07340 (NW967_07340) comGD 1555594..1556040 (-) 447 WP_258411171.1 competence type IV pilus minor pilin ComGD Machinery gene
  NW967_RS07345 (NW967_07345) comGC 1556018..1556329 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  NW967_RS07350 (NW967_07350) comGB 1556343..1557413 (-) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  NW967_RS07355 (NW967_07355) comGA 1557385..1558359 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  NW967_RS07360 (NW967_07360) - 1558411..1559034 (-) 624 WP_164002626.1 MBL fold metallo-hydrolase -
  NW967_RS07365 (NW967_07365) - 1559031..1559360 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  NW967_RS07370 (NW967_07370) - 1559360..1560346 (-) 987 WP_000161314.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19167.92 Da        Isoelectric Point: 10.2192

>NTDB_id=720208 NW967_RS07330 WP_258411170.1 1554893..1555390(-) (comGF) [Staphylococcus aureus strain 39-B-49]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLVEMLIAMMVISIILLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYA

Nucleotide


Download         Length: 498 bp        

>NTDB_id=720208 NW967_RS07330 WP_258411170.1 1554893..1555390(-) (comGF) [Staphylococcus aureus strain 39-B-49]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTGTTGAAATGTTAATAGCGATGATGGTTATAAGTATAATTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTTATTGAAAGTAGGGAATTAACAACTGTAGATTTTGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGCATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(69-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

97.561

99.394

0.97
  comGF Staphylococcus aureus MW2

96.341

99.394

0.958