Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   PT746_RS00190 Genome accession   NZ_CP117974
Coordinates   32237..32701 (+) Length   154 a.a.
NCBI ID   WP_021205141.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain B-3509     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 27237..37701
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PT746_RS00165 yacG 27407..27607 (-) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  PT746_RS00170 coaE 27604..28215 (-) 612 WP_003112838.1 dephospho-CoA kinase -
  PT746_RS00175 pilD 28212..29084 (-) 873 WP_003107301.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  PT746_RS00180 pilC 29085..30302 (-) 1218 WP_003161763.1 type II secretion system F family protein Machinery gene
  PT746_RS00185 pilB 30306..32006 (-) 1701 WP_021205142.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PT746_RS00190 pilA/pilAI 32237..32701 (+) 465 WP_021205141.1 pilin Machinery gene
  PT746_RS00195 - 32783..34162 (+) 1380 WP_223719891.1 O-antigen ligase family protein -
  PT746_RS00205 nadC 34343..35191 (-) 849 WP_003161760.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16076.44 Da        Isoelectric Point: 9.0145

>NTDB_id=719962 PT746_RS00190 WP_021205141.1 32237..32701(+) (pilA/pilAI) [Pseudomonas aeruginosa strain B-3509]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQDYTARTQVTRAVSEVSALKTAAESAILEGKEIVSSKTPTKDQYDIGFTG
STLLKGSEKGQITVKNGTLPTVQLEATLDGSSGAAIKGAVITVSRTADGVWGCSISSTPANWKPNYAPSNCPKS

Nucleotide


Download         Length: 465 bp        

>NTDB_id=719962 PT746_RS00190 WP_021205141.1 32237..32701(+) (pilA/pilAI) [Pseudomonas aeruginosa strain B-3509]
ATGAAAGCTCAGAAGGGTTTTACTCTGATCGAACTGATGATCGTGGTCGCGATCATCGGCATCCTGGCCGCCATTGCCAT
CCCGCAATACCAGGACTACACCGCCCGTACCCAGGTGACCCGTGCTGTGAGTGAAGTCAGCGCGCTGAAGACCGCTGCGG
AGTCGGCGATTCTGGAAGGGAAAGAAATTGTTTCCAGTAAGACTCCTACCAAGGACCAATATGATATTGGATTTACCGGT
TCTACCTTGCTGAAAGGGTCGGAGAAAGGTCAGATTACTGTGAAAAATGGTACTCTGCCTACTGTGCAGTTGGAAGCTAC
TCTGGATGGTTCTTCCGGTGCCGCTATTAAAGGTGCAGTTATTACCGTTTCGCGTACCGCTGATGGGGTGTGGGGTTGCT
CGATCAGTAGCACTCCTGCTAACTGGAAACCAAACTATGCCCCCTCTAACTGCCCGAAATCCTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

47.945

94.805

0.455
  pilA Pseudomonas aeruginosa PAK

42.857

100

0.448
  pilA Acinetobacter baumannii strain A118

45.205

94.805

0.429
  comP Acinetobacter baylyi ADP1

39.264

100

0.416
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.776

95.455

0.37
  pilA Vibrio parahaemolyticus RIMD 2210633

41.007

90.26

0.37