Detailed information NTG0719727

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: NWF33_RS20845
• Genome accession: NZ_CP102921
• Coordinates: 4241863..4242663 (+)
• Length: 266 a.a.
• NCBI ID: WP_017901157.1
• Uniprot ID: -
• Organism: Klebsiella quasipneumoniae strain K18-45
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4236863..4247663

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NWF33_RS20810 (NWF33_20810)	gspH	4237016..4237531 (+)	516	WP_017901154.1	type II secretion system minor pseudopilin GspH	-
NWF33_RS20815 (NWF33_20815)	gspI	4237528..4237893 (+)	366	WP_004204415.1	type II secretion system minor pseudopilin GspI	-
NWF33_RS20820 (NWF33_20820)	gspJ	4237890..4238486 (+)	597	WP_017901155.1	type II secretion system minor pseudopilin GspJ	-
NWF33_RS20825 (NWF33_20825)	gspK	4238483..4239463 (+)	981	WP_117146351.1	type II secretion system minor pseudopilin GspK	-
NWF33_RS20830 (NWF33_20830)	gspL	4239456..4240652 (+)	1197	WP_004204411.1	type II secretion system protein GspL	-
NWF33_RS20835 (NWF33_20835)	-	4240645..4241130 (+)	486	WP_004204409.1	type II secretion system protein M	-
NWF33_RS20840 (NWF33_20840)	-	4241120..4241848 (+)	729	WP_004204408.1	type II secretion system protein N	-
NWF33_RS20845 (NWF33_20845)	pilD	4241863..4242663 (+)	801	WP_017901157.1	A24 family peptidase	Machinery gene
NWF33_RS20850 (NWF33_20850)	hrpB	4242711..4245140 (-)	2430	WP_017901158.1	ATP-dependent helicase HrpB	-
NWF33_RS20855 (NWF33_20855)	thpR	4245213..4245749 (+)	537	WP_117146352.1	RNA 2',3'-cyclic phosphodiesterase	-
NWF33_RS20860 (NWF33_20860)	sfsA	4245749..4246465 (+)	717	WP_004204404.1	DNA/RNA nuclease SfsA	-
NWF33_RS20865 (NWF33_20865)	dksA	4246628..4247083 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29338.86 Da
• Isoelectric Point: 8.8894

>NTDB_id=719727 NWF33_RS20845 WP_017901157.1 4241863..4242663(+) (pilD) [Klebsiella quasipneumoniae strain K18-45]
MTTLAALSLHFPLVWYGFLLLFGLALGSFYNVVIYRLPRMLAQTADDEPLTLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQARISWSYPLTELTTGLLFILAGALLAPGLALAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQALPQVLLFASASGLTWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGVFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=719727 NWF33_RS20845 WP_017901157.1 4241863..4242663(+) (pilD) [Klebsiella quasipneumoniae strain K18-45]
ATGACGACATTAGCCGCATTATCGCTGCATTTCCCCCTTGTCTGGTATGGCTTTCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAACGTGGTGATCTACCGTCTGCCGCGCATGCTGGCCCAGACCGCGGACGACGAGCCGCTAACCCTCAGCA
CGCCGGGCTCATCCTGTCCGCAGTGTCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGCTTCCTCTGGCTA
GGCCGTCGCGCCCGCTGCTGTCAGGCGCGCATCTCGTGGAGCTATCCGCTGACCGAGCTGACCACCGGCCTGCTGTTTAT
CCTCGCGGGGGCGCTGCTCGCACCGGGACTGGCGCTGGCGGGCGGCCTGGTGCTGCTCTCCTTTCTGCTGATACTGGCGC
GGATTGATGCCCGGACCCAGCTGCTGCCCGATCGCCTGACCCTGCCCCTGCTGTGGGCCGGACTGCTGTTTAATCTTAAC
GAGGTGTACATTGCGCTGCCGGACGCGGTCGCCGGGGCGATGGCTGGTTATCTGGCACTGTGGTCGGTCTATTGGCTGTT
CCGCCTGCTGACCGGCAAAGAGGCTCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGCTCTGCCGCAGGTGCTGTTGTTCGCCTCGGCCAGCGGGCTGACGTGGACCCTGCTGCAGCGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCGGGAGGGGGCGTTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (123-230)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	45.818	100	0.474
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	44.867	98.872	0.444
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425