Detailed information NTG0719548

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: NWT79_RS21070
• Genome accession: NZ_CP102889
• Coordinates: 4312104..4312904 (+)
• Length: 266 a.a.
• NCBI ID: WP_004213441.1
• Uniprot ID: A0A9Q4U0T4
• Organism: Klebsiella pneumoniae strain KW2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4307104..4317904

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NWT79_RS21035 (NWT79_21035)	gspH	4307260..4307775 (+)	516	WP_004191951.1	type II secretion system minor pseudopilin GspH	-
NWT79_RS21040 (NWT79_21040)	gspI	4307772..4308137 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
NWT79_RS21045 (NWT79_21045)	gspJ	4308134..4308730 (+)	597	WP_004183043.1	type II secretion system minor pseudopilin GspJ	-
NWT79_RS21050 (NWT79_21050)	gspK	4308727..4309713 (+)	987	WP_004178610.1	type II secretion system minor pseudopilin GspK	-
NWT79_RS21055 (NWT79_21055)	gspL	4309700..4310893 (+)	1194	WP_080792608.1	type II secretion system protein GspL	-
NWT79_RS21060 (NWT79_21060)	-	4310886..4311371 (+)	486	WP_058229142.1	type II secretion system protein M	-
NWT79_RS21065 (NWT79_21065)	-	4311361..4312107 (+)	747	WP_031588870.1	type II secretion system protein N	-
NWT79_RS21070 (NWT79_21070)	pilD	4312104..4312904 (+)	801	WP_004213441.1	A24 family peptidase	Machinery gene
NWT79_RS21075 (NWT79_21075)	hrpB	4312952..4315381 (-)	2430	WP_080792605.1	ATP-dependent helicase HrpB	-
NWT79_RS21080 (NWT79_21080)	thpR	4315454..4315990 (+)	537	WP_004177389.1	RNA 2',3'-cyclic phosphodiesterase	-
NWT79_RS21085 (NWT79_21085)	sfsA	4315990..4316706 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
NWT79_RS21090 (NWT79_21090)	dksA	4316869..4317324 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29405.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=719548 NWT79_RS21070 WP_004213441.1 4312104..4312904(+) (pilD) [Klebsiella pneumoniae strain KW2]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPISWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=719548 NWT79_RS21070 WP_004213441.1 4312104..4312904(+) (pilD) [Klebsiella pneumoniae strain KW2]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGTATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCTCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCAGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425