Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   PT012_RS05125 Genome accession   NZ_CP117820
Coordinates   1134765..1136465 (-) Length   566 a.a.
NCBI ID   WP_027700575.1    Uniprot ID   -
Organism   Xylella fastidiosa subsp. multiplex strain ICMP 8740     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1129765..1141465
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PT012_RS05095 (PT012_05095) - 1130351..1131196 (-) 846 WP_004083682.1 type II secretion system protein GspK -
  PT012_RS05100 (PT012_05100) - 1131196..1131822 (-) 627 WP_004083683.1 type II secretion system protein J -
  PT012_RS05105 (PT012_05105) - 1131819..1132217 (-) 399 WP_004083684.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  PT012_RS05110 (PT012_05110) - 1132214..1132720 (-) 507 WP_004083685.1 Tfp pilus assembly protein FimT/FimU -
  PT012_RS05115 (PT012_05115) gspG 1132726..1133160 (-) 435 WP_004083686.1 type II secretion system major pseudopilin GspG -
  PT012_RS05120 (PT012_05120) - 1133512..1134729 (-) 1218 WP_027700574.1 type II secretion system F family protein -
  PT012_RS05125 (PT012_05125) pilF 1134765..1136465 (-) 1701 WP_027700575.1 type II secretion system ATPase GspE Machinery gene
  PT012_RS05130 (PT012_05130) - 1136758..1139718 (-) 2961 WP_272819563.1 YadA-like family protein -
  PT012_RS05135 (PT012_05135) - 1140383..1140742 (-) 360 WP_004084115.1 AEC family transporter -
  PT012_RS05140 (PT012_05140) - 1140776..1141294 (-) 519 WP_004084214.1 hypothetical protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62711.56 Da        Isoelectric Point: 5.0845

>NTDB_id=719443 PT012_RS05125 WP_027700575.1 1134765..1136465(-) (pilF) [Xylella fastidiosa subsp. multiplex strain ICMP 8740]
MNVSMEASIVESLLQRRCLKESDLYRARQLQAESGVGLLPLLGRLGLVSERDHAEVCAEVMGLLLIQVRELPDTPPEFSA
EVHGLSIRFLKQFHVCPLAEHDGVLQLCVADPFDVYAIEALRLATGAVLQLCIGLRSEIDDLVERWYGQGRSVMGMIVEA
TDGDAAPADDIEALRDLASEAPVIRLVNLVIQRAVELRASDIHIEPFENRLKVRYRVDGVLIDAESPPSKLAAAVVSRVK
IMAKLNIAERRLPQDGRIMLRVQGKELDLRVSVVPTSHGESVVMRLLDRETVVFDFQKLGFTDELLNRFSRVLEMPHGIL
LVTGPTGSGKTTTLYTVLNRLNTTGVKIITVEDPVEYQIEGINQIQAKPQIGLDFASALRSIVRQDPDIIMIGEMRDLET
ARIAIQSALTGHLVLSTLHTNSAAGGLTRLLDMGVEDYLLTSTINGVLAQRLVRRLEPMHAERYLASPEQIARFDLRRLQ
PEGDIYLYRPRASALAPTGYLGRTAIVEFLIMDDVLRRAVISRTGIGELEQIARQAGMRTMYEDGLIKALAGETTIEEVL
RVTEEG

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=719443 PT012_RS05125 WP_027700575.1 1134765..1136465(-) (pilF) [Xylella fastidiosa subsp. multiplex strain ICMP 8740]
ATGAATGTAAGTATGGAAGCATCCATCGTTGAGTCGTTGTTGCAGCGTCGTTGCTTGAAAGAAAGTGATCTGTATCGCGC
GCGTCAGTTGCAGGCCGAATCCGGTGTTGGGTTGTTACCTTTGTTGGGCCGTCTTGGTTTGGTTTCTGAGCGCGATCACG
CTGAAGTATGTGCGGAGGTGATGGGGCTGTTGTTGATACAGGTGCGTGAATTGCCGGATACGCCGCCAGAATTCTCGGCG
GAGGTGCATGGGTTGTCCATTCGCTTTCTCAAACAGTTCCATGTATGCCCGCTTGCTGAGCATGATGGTGTGTTGCAGTT
GTGTGTTGCAGATCCATTTGATGTGTACGCCATTGAGGCGTTGCGCCTTGCCACAGGTGCAGTATTGCAATTGTGTATCG
GTTTGCGTTCCGAAATTGATGACTTGGTCGAGCGCTGGTATGGCCAAGGCCGCAGCGTCATGGGCATGATTGTGGAGGCG
ACCGATGGTGATGCGGCGCCCGCAGATGACATTGAGGCTTTGCGTGATCTGGCGTCTGAAGCGCCTGTGATCCGGCTGGT
GAACTTGGTGATTCAGCGTGCGGTTGAATTACGCGCCTCTGATATCCATATCGAACCATTCGAGAATCGGTTGAAGGTAC
GTTATCGCGTCGATGGCGTGTTGATTGATGCGGAGAGTCCGCCATCTAAATTGGCTGCTGCTGTGGTTTCACGTGTCAAG
ATTATGGCCAAACTTAATATTGCTGAACGCCGCTTGCCCCAGGATGGGCGAATCATGTTGCGTGTTCAGGGTAAAGAGCT
GGATCTGCGGGTGAGTGTTGTGCCGACTTCACATGGTGAAAGCGTCGTGATGCGCCTGCTTGACCGGGAGACGGTGGTAT
TCGATTTTCAGAAGCTTGGTTTTACCGATGAGTTATTAAACAGGTTCTCCCGTGTGTTGGAGATGCCTCATGGCATTTTA
CTGGTCACGGGTCCGACGGGATCTGGAAAGACCACCACGCTGTATACGGTGTTAAATCGTCTCAATACTACCGGTGTCAA
GATTATTACGGTGGAAGATCCGGTTGAGTATCAGATTGAAGGCATCAATCAGATCCAGGCGAAGCCGCAGATCGGGCTGG
ATTTTGCCAGCGCACTACGCAGCATTGTGCGTCAGGATCCGGACATCATCATGATTGGTGAAATGCGGGATCTGGAAACT
GCGCGCATTGCTATCCAGTCCGCGCTGACGGGGCATTTGGTGCTGTCTACTTTGCATACCAATAGTGCTGCCGGTGGGCT
GACCCGATTACTGGATATGGGTGTGGAGGATTATTTGTTGACCTCCACAATCAATGGTGTTTTGGCGCAGCGTTTGGTGC
GTCGCTTGGAGCCTATGCATGCTGAGCGTTATCTTGCTTCGCCAGAGCAGATCGCGCGTTTTGATTTGCGCCGTTTACAG
CCAGAGGGTGATATTTATTTGTACCGTCCGCGCGCTTCAGCGCTTGCGCCCACTGGGTATCTGGGCCGTACGGCGATCGT
CGAGTTTCTGATTATGGATGATGTGCTGCGTCGCGCGGTGATCAGCCGTACCGGCATAGGGGAGTTGGAGCAGATCGCAC
GTCAAGCAGGGATGCGTACTATGTACGAGGACGGTCTCATCAAGGCATTGGCTGGTGAGACTACAATTGAAGAGGTCTTG
CGCGTGACGGAGGAAGGCTGA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-151)

Type II/IV secretion system protein (T2SSE)

(189-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

40.761

97.527

0.398
  pilB Vibrio campbellii strain DS40M4

40.516

95.936

0.389
  pilB Vibrio cholerae strain A1552

38.38

100

0.385
  pilB Vibrio parahaemolyticus RIMD 2210633

40.074

95.23

0.382
  pilB Legionella pneumophila strain ERS1305867

36.731

100

0.369