Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   NWE25_RS12495 Genome accession   NZ_CP102866
Coordinates   2618421..2619035 (-) Length   204 a.a.
NCBI ID   WP_038459268.1    Uniprot ID   -
Organism   Bacillus velezensis strain YS-AT-DS1     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2613421..2624035
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NWE25_RS12465 (NWE25_12465) rpsT 2613724..2613990 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  NWE25_RS12470 (NWE25_12470) holA 2614007..2615048 (-) 1042 Protein_2416 DNA polymerase III subunit delta -
  NWE25_RS12475 (NWE25_12475) - 2615088..2615239 (-) 152 Protein_2417 hypothetical protein -
  NWE25_RS12480 (NWE25_12480) - 2615280..2615414 (+) 135 WP_003152870.1 YqzM family protein -
  NWE25_RS12485 (NWE25_12485) comEC 2615433..2617784 (-) 2352 WP_038459264.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  NWE25_RS12490 (NWE25_12490) - 2617785..2618354 (-) 570 WP_038459266.1 ComE operon protein 2 -
  NWE25_RS12495 (NWE25_12495) comEA 2618421..2619035 (-) 615 WP_038459268.1 helix-hairpin-helix domain-containing protein Machinery gene
  NWE25_RS12500 (NWE25_12500) comER 2619094..2619915 (+) 822 WP_012118027.1 late competence protein ComER -
  NWE25_RS12505 (NWE25_12505) - 2619984..2620721 (-) 738 WP_038459270.1 class I SAM-dependent methyltransferase -
  NWE25_RS12510 (NWE25_12510) rsfS 2620718..2621074 (-) 357 WP_007408260.1 ribosome silencing factor -
  NWE25_RS12515 (NWE25_12515) yqeK 2621078..2621653 (-) 576 WP_032873949.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  NWE25_RS12520 (NWE25_12520) - 2621643..2622212 (-) 570 WP_007408258.1 nicotinate-nucleotide adenylyltransferase -
  NWE25_RS12525 (NWE25_12525) yhbY 2622223..2622513 (-) 291 WP_007408257.1 ribosome assembly RNA-binding protein YhbY -
  NWE25_RS12530 (NWE25_12530) aroE 2622507..2623349 (-) 843 WP_003152856.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22206.44 Da        Isoelectric Point: 6.4939

>NTDB_id=719434 NWE25_RS12495 WP_038459268.1 2618421..2619035(-) (comEA) [Bacillus velezensis strain YS-AT-DS1]
MTKAMMTEPLRRNLKILGACLVAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELKVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREEKGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=719434 NWE25_RS12495 WP_038459268.1 2618421..2619035(-) (comEA) [Bacillus velezensis strain YS-AT-DS1]
ATGACGAAAGCGATGATGACGGAGCCGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGTAGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGACAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACTCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAAAGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAAAGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(68-122)

Helix-hairpin-helix motif (HHH_3)

(141-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.115

93.627

0.544
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

35.071

100

0.363