Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   PSR34_RS22455 Genome accession   NZ_CP117672
Coordinates   4605114..4607312 (+) Length   732 a.a.
NCBI ID   WP_001298972.1    Uniprot ID   -
Organism   Escherichia coli strain KW21     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4600114..4612312
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSR34_RS22430 (PSR34_22430) metB 4601592..4602752 (-) 1161 WP_001297633.1 cystathionine gamma-synthase -
  PSR34_RS22435 (PSR34_22435) metJ 4603029..4603346 (+) 318 WP_000852812.1 met regulon transcriptional regulator MetJ -
  PSR34_RS22440 (PSR34_22440) yiiX 4603406..4604014 (+) 609 WP_001402675.1 YiiX family permuted papain-like enzyme -
  PSR34_RS22445 (PSR34_22445) - 4604021..4604644 (+) 624 WP_001402674.1 TIGR02117 family protein -
  PSR34_RS22450 (PSR34_22450) rpmE 4604699..4604911 (-) 213 WP_000710769.1 50S ribosomal protein L31 -
  PSR34_RS22455 (PSR34_22455) priA 4605114..4607312 (+) 2199 WP_001298972.1 primosomal protein N' Machinery gene
  PSR34_RS22460 (PSR34_22460) cytR 4607468..4608493 (+) 1026 WP_000644904.1 DNA-binding transcriptional regulator CytR Regulator
  PSR34_RS22465 (PSR34_22465) ftsN 4608585..4609544 (+) 960 WP_000068834.1 cell division protein FtsN -
  PSR34_RS22470 (PSR34_22470) hslV 4609637..4610167 (+) 531 WP_000208242.1 ATP-dependent protease subunit HslV -
  PSR34_RS22475 (PSR34_22475) hslU 4610177..4611508 (+) 1332 WP_001293343.1 HslU--HslV peptidase ATPase subunit -

Sequence

Protein


Download         Length: 732 a.a.        Molecular weight: 81659.93 Da        Isoelectric Point: 8.9077

>NTDB_id=718488 PSR34_RS22455 WP_001298972.1 4605114..4607312(+) (priA) [Escherichia coli strain KW21]
MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIGVVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRL
LLWAADYYHHPIGDVLFHALPILLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
NDAALQALRKKGLCDLASETPEFSDWQTNYAVSGERLRLNTEQATAVGAIHSAADTFSAWLLAGVTGSGKTEVYLSVLEN
VLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVLHSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVID
EEHDSSYKQQEGWRYHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVLDLKGQKVQ
AGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDHYYTLHQAQQHLRCHHCDSQRPVPRQCPSCG
STHLVPVGLGTEQLEQTLAPLFPDVPISRIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVD
GALFSADFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAERRMMQLPPWTSHVIVR
AEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPKRGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRK
VKWVLDVDPIEG

Nucleotide


Download         Length: 2199 bp        

>NTDB_id=718488 PSR34_RS22455 WP_001298972.1 4605114..4607312(+) (priA) [Escherichia coli strain KW21]
ATGCCCGTTGCCCACGTTGCCTTGCCCGTTCCGCTTCCTCGTACCTTTGACTATCTGCTGCCAGAAGGCATGACGGTTAA
AGCCGGGTGTCGCGTGCGCGTGCCGTTTGGCAAACAGCAGGAGCGCATCGGGGTTGTGGTGTCAGTTAGCGATGTCAGTG
AGCTGCCGCTTAACGAGCTGAAAGCGGTAGTGGAAGTGCTGGACGTTGAGCCGGTATTTACTCACTCCGTCTGGCGGTTA
CTGCTCTGGGCGGCAGATTACTATCATCATCCGATTGGCGATGTGCTGTTTCATGCCTTGCCGATTTTACTGCGCCAGGG
GCGGCCTGCGGCGAACGCGCCGATGTGGTACTGGTTTGCCACTGAACAAGGCCAGGCGGTGGATCTGAACAGCCTGAAAC
GCTCCCCCAAGCAACAACAGGCGCTGGCGGCGTTACGGCAAGGCAAAATCTGGCGCGACCAGGTCGCCACGCTCGAATTT
AATGATGCCGCGTTGCAGGCGCTACGCAAAAAAGGTCTGTGTGATTTAGCAAGTGAAACACCAGAGTTTAGCGACTGGCA
AACGAACTATGCCGTTTCTGGTGAGCGGTTGCGATTGAATACCGAACAGGCCACCGCCGTTGGCGCAATTCATAGCGCGG
CAGATACTTTTTCTGCCTGGCTGCTGGCGGGCGTTACCGGTTCCGGTAAAACGGAGGTTTATCTCAGTGTACTGGAAAAC
GTGCTCGCTCAGGGCAAACAGGCGCTGGTGATGGTGCCGGAAATCGGCCTGACACCGCAAACTATCGCCCGTTTTCGTGA
ACGTTTTAATGCCCCCGTGGAAGTTCTGCATTCCGGCCTGAACGACAGCGAGCGGCTTTCGGCGTGGTTGAAAGCGAAAA
ATGGCGAGGCGGCGATTGTGATCGGCACCCGCTCCGCGCTGTTTACGCCGTTTAAAAATCTCGGCGTGATTGTCATTGAT
GAAGAGCACGACAGCTCCTACAAGCAGCAGGAAGGCTGGCGCTATCATGCCCGCGACCTGGCGGTGTATCGTGCGCATAG
CGAGCAAATCCCGATTATTCTTGGCTCCGCAACGCCCGCGCTGGAAACGTTGTGCAACGTCCAGCAGAAAAAATACCGCC
TGCTGCGCCTGACGCGCCGGGCGGGGAATGCGCGTCCGGCAATTCAACACGTGCTGGATTTAAAAGGTCAGAAGGTGCAG
GCAGGTCTGGCTCCGGCGTTAATCACCCGTATGCGCCAGCATTTACAGGCCAACAATCAGGTTATCCTCTTTCTTAATCG
TCGGGGTTTTGCGCCTGCGCTACTGTGCCACGACTGCGGCTGGATTGCCGAATGCCCACGTTGCGATCACTACTACACGC
TGCATCAGGCGCAACAGCATTTGCGCTGTCACCACTGTGACAGTCAGCGTCCGGTGCCGCGCCAGTGCCCTTCCTGCGGT
TCCACGCACCTGGTACCCGTGGGGCTGGGCACCGAACAGCTTGAACAGACGCTTGCGCCGCTGTTCCCTGATGTGCCCAT
TTCTCGTATCGACCGCGATACCACCAGCCGCAAAGGGGCGCTGGAACAGCAACTGGCGGAAGTACATCGCGGCGGTGCGA
GGATTTTGATCGGCACACAAATGCTGGCGAAAGGTCACCACTTTCCGGATGTGACGCTGGTGGCGTTACTGGATGTGGAC
GGCGCGCTGTTTTCTGCCGATTTTCGCTCGGCAGAGCGTTTCGCTCAGCTTTACACCCAGGTCGCTGGTCGTGCCGGGCG
TGCGGGTAAACAAGGCGAAGTGGTGCTGCAAACGCACCATCCAGAACATCCTCTGTTGCAAACGTTGCTCTATAAAGGCT
ACGACGCCTTTGCCGAACAGGCGCTGGCTGAGCGACGGATGATGCAACTGCCGCCATGGACCAGCCATGTGATTGTGCGT
GCGGAAGATCATAACAATCAGCATGCGCCGCTGTTCCTGCAACAACTGCGTAATCTGATCCTCTCCAGCCCGCTGGCAGA
CGACAAACTGTGGGTTCTCGGTCCGGTTCCGGCACTGGCACCTAAACGTGGCGGTCGCTGGCGCTGGCAGATATTGTTGC
AGCACCCTTCCCGCGTGCGCTTGCAACACATCATTAGTGGTACGCTGGCGCTCATCAATACAATACCGGATTCCCGCAAG
GTGAAATGGGTACTGGATGTTGATCCGATTGAGGGTTAA

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(203-362)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(445-471)

3DNA-binding domain (3BD) (PriA_3primeBD)

(6-101)

Primosomal protein N C-terminal domain (PriA_C)

(632-729)

Helicase conserved C-terminal domain (Helicase_C)

(522-589)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

44.912

100

0.452