Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   K6J59_RS03395 Genome accession   NZ_AP018782
Coordinates   685696..686238 (-) Length   180 a.a.
NCBI ID   WP_005666612.1    Uniprot ID   -
Organism   Haemophilus influenzae strain CHBN-V-2 isolate 13H37     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 680696..691238
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6J59_RS03365 (CHBNV2_06340) fdx 681099..681440 (+) 342 WP_005656373.1 ISC system 2Fe-2S type ferredoxin -
  K6J59_RS03370 (CHBNV2_06350) iscX 681440..681634 (+) 195 WP_005659903.1 Fe-S cluster assembly protein IscX -
  K6J59_RS03375 (CHBNV2_06360) - 681685..682299 (-) 615 WP_005666604.1 YfgM family protein -
  K6J59_RS03380 (CHBNV2_06370) hisS 682317..683588 (-) 1272 WP_005666606.1 histidine--tRNA ligase -
  K6J59_RS03385 (CHBNV2_06380) ispG 683598..684704 (-) 1107 WP_005666608.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  K6J59_RS03390 (CHBNV2_06390) - 684713..685624 (-) 912 WP_005666610.1 RodZ family helix-turn-helix domain-containing protein -
  K6J59_RS03395 (CHBNV2_06400) pilF/pilF2 685696..686238 (-) 543 WP_005666612.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  K6J59_RS03400 (CHBNV2_06410) - 686339..687490 (-) 1152 WP_005666614.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  K6J59_RS03405 (CHBNV2_06420) - 687737..688615 (+) 879 WP_005649130.1 serine hydrolase -
  K6J59_RS03410 (CHBNV2_06430) - 688761..689897 (-) 1137 WP_005666616.1 RNA-guided endonuclease TnpB family protein -
  K6J59_RS03415 (CHBNV2_06440) - 690272..691153 (+) 882 WP_110434976.1 metal ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 180 a.a.        Molecular weight: 20807.49 Da        Isoelectric Point: 8.6167

>NTDB_id=71355 K6J59_RS03395 WP_005666612.1 685696..686238(-) (pilF/pilF2) [Haemophilus influenzae strain CHBN-V-2 isolate 13H37]
MQTISKQLSAVIFPFIFSACVSQSTIQDFNQQSAAKARVELALSYLQQNNPQLAKINLDKALQHDKNYYLVHSALAHYYQ
QQGQIENAFREYEIAVKLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQADTFENIALCAYSAKKMDIYQQ
TLEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 543 bp        

>NTDB_id=71355 K6J59_RS03395 WP_005666612.1 685696..686238(-) (pilF/pilF2) [Haemophilus influenzae strain CHBN-V-2 isolate 13H37]
ATGCAAACAATAAGTAAACAACTAAGTGCGGTTATTTTCCCTTTCATTTTTTCTGCTTGCGTTTCCCAATCAACTATTCA
AGACTTCAATCAACAATCAGCAGCGAAAGCTCGCGTAGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTGG
CTAAAATCAATTTAGACAAAGCACTTCAACACGATAAAAATTACTATCTCGTACATTCAGCACTTGCACATTATTATCAA
CAACAAGGGCAAATAGAAAATGCCTTTCGTGAGTATGAAATAGCCGTAAAACTTAATCATAAACAAGGCGATGTACATAA
TAATTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAGCAAGCCCAGCAACAATTTGAATTAGCCCTTAATTCGCCGA
ATTATTATCATCAAGCAGATACATTTGAAAATATCGCGCTTTGTGCTTATTCCGCGAAAAAAATGGATATTTATCAGCAA
ACATTAGAAAAATTACGTCAAATCGATGGAAAACGTGCGGAAAAATTCAATAGCCTAAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

93.889

100

0.939
  pilF Glaesserella parasuis strain SC1401

48.045

99.444

0.478

Multiple sequence alignment