Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   CHBNTHi_RS03705 Genome accession   NZ_AP018781
Coordinates   731045..731584 (-) Length   179 a.a.
NCBI ID   WP_005659915.1    Uniprot ID   -
Organism   Haemophilus influenzae strain CHBN-V-1 isolate 13H43     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 726045..736584
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CHBNTHi_RS03675 (CHBNV1_07050) fdx 726448..726789 (+) 342 WP_005656373.1 ISC system 2Fe-2S type ferredoxin -
  CHBNTHi_RS03680 (CHBNV1_07060) iscX 726789..726983 (+) 195 WP_005656371.1 Fe-S cluster assembly protein IscX -
  CHBNTHi_RS03685 (CHBNV1_07070) - 727034..727648 (-) 615 WP_005654851.1 YfgM family protein -
  CHBNTHi_RS03690 (CHBNV1_07080) hisS 727666..728937 (-) 1272 WP_005662656.1 histidine--tRNA ligase -
  CHBNTHi_RS03695 (CHBNV1_07090) ispG 728947..730053 (-) 1107 WP_105897010.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  CHBNTHi_RS03700 (CHBNV1_07100) - 730062..730973 (-) 912 WP_110437692.1 RodZ family helix-turn-helix domain-containing protein -
  CHBNTHi_RS03705 (CHBNV1_07110) pilF/pilF2 731045..731584 (-) 540 WP_005659915.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  CHBNTHi_RS03710 (CHBNV1_07120) - 731685..732836 (-) 1152 WP_005659917.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  CHBNTHi_RS03715 (CHBNV1_07130) - 733083..733961 (+) 879 WP_005659920.1 D-alanyl-D-alanine carboxypeptidase family protein -
  CHBNTHi_RS03720 (CHBNV1_07140) - 734326..735207 (+) 882 WP_005656358.1 metal ABC transporter substrate-binding protein -
  CHBNTHi_RS03725 (CHBNV1_07150) - 735207..736127 (+) 921 WP_005630123.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20602.19 Da        Isoelectric Point: 8.2626

>NTDB_id=71319 CHBNTHi_RS03705 WP_005659915.1 731045..731584(-) (pilF/pilF2) [Haemophilus influenzae strain CHBN-V-1 isolate 13H43]
MKTISKQLSAVIFPFIFSACVSQSASNLNHQAAAKARVELALSYLQQNNPQLAKINLDKALQHDKNYYLVHSALAHYYQQ
QGEIENAHREYEIAVNLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQADTFENIVLCAYSSQKMDIYQQT
LEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=71319 CHBNTHi_RS03705 WP_005659915.1 731045..731584(-) (pilF/pilF2) [Haemophilus influenzae strain CHBN-V-1 isolate 13H43]
ATGAAAACAATAAGTAAACAACTAAGTGCGGTTATTTTCCCTTTCATTTTTTCTGCCTGCGTTTCACAATCTGCATCTAA
CTTAAATCATCAAGCTGCCGCTAAAGCGCGTGTGGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTGGCTA
AAATCAATTTAGACAAAGCACTTCAACACGATAAAAATTACTATCTCGTGCATTCAGCACTTGCACATTATTATCAACAA
CAAGGGGAAATAGAGAATGCACATCGTGAATATGAAATAGCCGTAAACCTTAATCATAAACAAGGCGATGTGCATAATAA
TTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAACAAGCCCAGCAACAATTTGAATTAGCACTTAATTCGCCGAATT
ATTATCATCAAGCAGATACATTTGAAAATATCGTGCTTTGTGCTTATTCCTCGCAAAAAATGGATATTTATCAGCAAACT
TTAGAAAAATTACGTCAAATCGATGGAAAACGTGCGGAAAAATTCAATAGTCTAAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

97.207

100

0.972
  pilF Glaesserella parasuis strain SC1401

44.693

100

0.447

Multiple sequence alignment