Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   NP436_RS01970 Genome accession   NZ_CP101932
Coordinates   369602..370051 (-) Length   149 a.a.
NCBI ID   WP_014475770.1    Uniprot ID   A0A0D1KPR3
Organism   Bacillus subtilis subsp. natto strain BGSC 27E1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 364602..375051
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NP436_RS01930 (NP436_01930) - 364927..365124 (+) 198 WP_121509395.1 hypothetical protein -
  NP436_RS01935 (NP436_01935) yckC 365144..365599 (+) 456 WP_014478793.1 RDD family protein -
  NP436_RS01940 (NP436_01940) yckD 365681..366013 (+) 333 WP_014478794.1 YckD family protein -
  NP436_RS01945 (NP436_01945) - 366141..366563 (+) 423 WP_014478795.1 MarR family winged helix-turn-helix transcriptional regulator -
  NP436_RS01950 (NP436_01950) - 366551..366871 (+) 321 WP_014478796.1 DUF3147 family protein -
  NP436_RS01955 (NP436_01955) - 366890..367634 (+) 745 Protein_341 AAA family ATPase -
  NP436_RS01960 (NP436_01960) bglC 367707..369140 (+) 1434 WP_014478799.1 6-phospho-beta-glucosidase -
  NP436_RS01965 (NP436_01965) nin/comJ 369177..369575 (-) 399 WP_014478800.1 DNA-entry nuclease inhibitor Regulator
  NP436_RS01970 (NP436_01970) nucA/comI 369602..370051 (-) 450 WP_014475770.1 DNA-entry nuclease Machinery gene
  NP436_RS01975 (NP436_01975) tlpC 370219..371940 (-) 1722 Protein_345 methyl-accepting chemotaxis protein TlpC -
  NP436_RS01980 (NP436_01980) hxlB 372051..372608 (-) 558 WP_014478803.1 6-phospho-3-hexuloisomerase -
  NP436_RS01985 (NP436_01985) hxlA 372614..373246 (-) 633 WP_014478804.1 3-hexulose-6-phosphate synthase -
  NP436_RS01990 (NP436_01990) hxlR 373475..373837 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16440.39 Da        Isoelectric Point: 4.5282

>NTDB_id=713128 NP436_RS01970 WP_014475770.1 369602..370051(-) (nucA/comI) [Bacillus subtilis subsp. natto strain BGSC 27E1]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=713128 NP436_RS01970 WP_014475770.1 369602..370051(-) (nucA/comI) [Bacillus subtilis subsp. natto strain BGSC 27E1]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATTGACAGAGACGGAGCT
GAAGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D1KPR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

98.658

100

0.987